Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic Escherichia coli O1

The effect of emodin on the intestinal mucosal barrier of a mouse E. coli O1-induced diarrhea model was observed. Following successful establishment of a diarrhea model, the mice were treated with drugs for seven days. Intestinal lesions and the shape and th...

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Autores principales:	Ruijuan Gao, Chunjie Wang, Aricha Han, Yanping Tian, Shunan Ren, Wenting Lv, Aorigele Chen, Jian Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	emodin <i>Escherichia coli</i> O<sub>1</sub> diarrhea intestinal mucosal barrier 16S rRNA sequencing Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/6a0da64e113240f0be95eabc909d0a2d
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spelling	oai:doaj.org-article:6a0da64e113240f0be95eabc909d0a2d2021-11-25T16:21:22ZEmodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>10.3390/ani111133142076-2615https://doaj.org/article/6a0da64e113240f0be95eabc909d0a2d2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3314https://doaj.org/toc/2076-2615The effect of emodin on the intestinal mucosal barrier of a mouse <i>E. coli</i> O<sub>1</sub>-induced diarrhea model was observed. Following successful establishment of a diarrhea model, the mice were treated with drugs for seven days. Intestinal lesions and the shape and the number of goblet cells were assessed via hematoxylin-eosin and periodic-acid-Schiff staining, while changes in inflammatory factors, ultrastructure of the small intestine, expression of MUC-2, and changes in the intestinal microbiota were analyzed via RT-PCR, electron microscopy, immunofluorescence, and 16S rRNA sequencing. Examination showed that emodin ameliorated pathological damage to the intestines of diarrheic mice. RT-PCR indicated that emodin reduced TNF-α, IL-β, IL-6, MPO, and COX-2 mRNA levels in duodenal tissues and increased the levels of sIgA and MUC-2 and the number of goblet cells. Microbiome analysis revealed that <i>Escherichia coli</i> O<sub>1</sub> reduced bacterial richness and altered the distribution pattern of bacterial communities at the phylum and order levels in cecum contents. Notably, pathogenic <i>Clostridiales</i> and <i>Enterobacteriales</i> were significantly increased in diarrheic mice. However, emodin reversed the trend. Thus, emodin protected against intestinal damage induced by <i>E. coli</i> O<sub>1</sub> and improved intestinal mucosal barrier function in mice by increasing the abundance of beneficial intestinal microbiota and inhibiting the abundance of harmful bacteria, thereby alleviating diarrhea.Ruijuan GaoChunjie WangAricha HanYanping TianShunan RenWenting LvAorigele ChenJian ZhangMDPI AGarticleemodin<i>Escherichia coli</i> O<sub>1</sub>diarrheaintestinal mucosal barrier16S rRNA sequencingVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3314, p 3314 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	emodin <i>Escherichia coli</i> O<sub>1</sub> diarrhea intestinal mucosal barrier 16S rRNA sequencing Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	emodin <i>Escherichia coli</i> O<sub>1</sub> diarrhea intestinal mucosal barrier 16S rRNA sequencing Veterinary medicine SF600-1100 Zoology QL1-991 Ruijuan Gao Chunjie Wang Aricha Han Yanping Tian Shunan Ren Wenting Lv Aorigele Chen Jian Zhang Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>
description	The effect of emodin on the intestinal mucosal barrier of a mouse <i>E. coli</i> O<sub>1</sub>-induced diarrhea model was observed. Following successful establishment of a diarrhea model, the mice were treated with drugs for seven days. Intestinal lesions and the shape and the number of goblet cells were assessed via hematoxylin-eosin and periodic-acid-Schiff staining, while changes in inflammatory factors, ultrastructure of the small intestine, expression of MUC-2, and changes in the intestinal microbiota were analyzed via RT-PCR, electron microscopy, immunofluorescence, and 16S rRNA sequencing. Examination showed that emodin ameliorated pathological damage to the intestines of diarrheic mice. RT-PCR indicated that emodin reduced TNF-α, IL-β, IL-6, MPO, and COX-2 mRNA levels in duodenal tissues and increased the levels of sIgA and MUC-2 and the number of goblet cells. Microbiome analysis revealed that <i>Escherichia coli</i> O<sub>1</sub> reduced bacterial richness and altered the distribution pattern of bacterial communities at the phylum and order levels in cecum contents. Notably, pathogenic <i>Clostridiales</i> and <i>Enterobacteriales</i> were significantly increased in diarrheic mice. However, emodin reversed the trend. Thus, emodin protected against intestinal damage induced by <i>E. coli</i> O<sub>1</sub> and improved intestinal mucosal barrier function in mice by increasing the abundance of beneficial intestinal microbiota and inhibiting the abundance of harmful bacteria, thereby alleviating diarrhea.
format	article
author	Ruijuan Gao Chunjie Wang Aricha Han Yanping Tian Shunan Ren Wenting Lv Aorigele Chen Jian Zhang
author_facet	Ruijuan Gao Chunjie Wang Aricha Han Yanping Tian Shunan Ren Wenting Lv Aorigele Chen Jian Zhang
author_sort	Ruijuan Gao
title	Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>
title_short	Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>
title_full	Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>
title_fullStr	Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>
title_full_unstemmed	Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>
title_sort	emodin improves intestinal health and immunity through modulation of gut microbiota in mice infected by pathogenic <i>escherichia coli</i> o<sub>1</sub>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6a0da64e113240f0be95eabc909d0a2d
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Emodin Improves Intestinal Health and Immunity through Modulation of Gut Microbiota in Mice Infected by Pathogenic <i>Escherichia coli</i> O<sub>1</sub>

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