Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

APEX-based proximity labeling allows capturing protein interaction dynamics but its high labeling background limits its utility for cytosolic proteins. Here, the authors develop more selective proximity labeling probes, enabling the APEX-based characterization of time-resolved cytosolic protein inte...

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Autores principales:	Mi Ke, Xiao Yuan, An He, Peiyuan Yu, Wendong Chen, Yu Shi, Tony Hunter, Peng Zou, Ruijun Tian
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6a106013d625450083626aaeed6b1d45
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spelling	oai:doaj.org-article:6a106013d625450083626aaeed6b1d452021-12-02T15:16:23ZSpatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics10.1038/s41467-020-20367-x2041-1723https://doaj.org/article/6a106013d625450083626aaeed6b1d452021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20367-xhttps://doaj.org/toc/2041-1723APEX-based proximity labeling allows capturing protein interaction dynamics but its high labeling background limits its utility for cytosolic proteins. Here, the authors develop more selective proximity labeling probes, enabling the APEX-based characterization of time-resolved cytosolic protein interactomes.Mi KeXiao YuanAn HePeiyuan YuWendong ChenYu ShiTony HunterPeng ZouRuijun TianNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mi Ke Xiao Yuan An He Peiyuan Yu Wendong Chen Yu Shi Tony Hunter Peng Zou Ruijun Tian Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
description	APEX-based proximity labeling allows capturing protein interaction dynamics but its high labeling background limits its utility for cytosolic proteins. Here, the authors develop more selective proximity labeling probes, enabling the APEX-based characterization of time-resolved cytosolic protein interactomes.
format	article
author	Mi Ke Xiao Yuan An He Peiyuan Yu Wendong Chen Yu Shi Tony Hunter Peng Zou Ruijun Tian
author_facet	Mi Ke Xiao Yuan An He Peiyuan Yu Wendong Chen Yu Shi Tony Hunter Peng Zou Ruijun Tian
author_sort	Mi Ke
title	Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_short	Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_full	Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_fullStr	Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_full_unstemmed	Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
title_sort	spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6a106013d625450083626aaeed6b1d45
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Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

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