Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

APEX-based proximity labeling allows capturing protein interaction dynamics but its high labeling background limits its utility for cytosolic proteins. Here, the authors develop more selective proximity labeling probes, enabling the APEX-based characterization of time-resolved cytosolic protein inte...

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Autores principales: Mi Ke, Xiao Yuan, An He, Peiyuan Yu, Wendong Chen, Yu Shi, Tony Hunter, Peng Zou, Ruijun Tian
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/6a106013d625450083626aaeed6b1d45
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