Kinetics of antigen expression and epitope presentation during virus infection.

Current knowledge about the dynamics of antigen presentation to T cells during viral infection is very poor despite being of fundamental importance to our understanding of anti-viral immunity. Here we use an advanced mass spectrometry method to simultaneously quantify the presentation of eight vacci...

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Autores principales:	Nathan P Croft, Stewart A Smith, Yik Chun Wong, Chor Teck Tan, Nadine L Dudek, Inge E A Flesch, Leon C W Lin, David C Tscharke, Anthony W Purcell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6a1915d21ff04a768d317385c5da648c
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spelling	oai:doaj.org-article:6a1915d21ff04a768d317385c5da648c2021-11-18T06:06:06ZKinetics of antigen expression and epitope presentation during virus infection.1553-73661553-737410.1371/journal.ppat.1003129https://doaj.org/article/6a1915d21ff04a768d317385c5da648c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23382674/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Current knowledge about the dynamics of antigen presentation to T cells during viral infection is very poor despite being of fundamental importance to our understanding of anti-viral immunity. Here we use an advanced mass spectrometry method to simultaneously quantify the presentation of eight vaccinia virus peptide-MHC complexes (epitopes) on infected cells and the amounts of their source antigens at multiple times after infection. The results show a startling 1000-fold range in abundance as well as strikingly different kinetics across the epitopes monitored. The tight correlation between onset of protein expression and epitope display for most antigens provides the strongest support to date that antigen presentation is largely linked to translation and not later degradation of antigens. Finally, we show a complete disconnect between the epitope abundance and immunodominance hierarchy of these eight epitopes. This study highlights the complexity of viral antigen presentation by the host and demonstrates the weakness of simple models that assume total protein levels are directly linked to epitope presentation and immunogenicity.Nathan P CroftStewart A SmithYik Chun WongChor Teck TanNadine L DudekInge E A FleschLeon C W LinDavid C TscharkeAnthony W PurcellPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 1, p e1003129 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Nathan P Croft Stewart A Smith Yik Chun Wong Chor Teck Tan Nadine L Dudek Inge E A Flesch Leon C W Lin David C Tscharke Anthony W Purcell Kinetics of antigen expression and epitope presentation during virus infection.
description	Current knowledge about the dynamics of antigen presentation to T cells during viral infection is very poor despite being of fundamental importance to our understanding of anti-viral immunity. Here we use an advanced mass spectrometry method to simultaneously quantify the presentation of eight vaccinia virus peptide-MHC complexes (epitopes) on infected cells and the amounts of their source antigens at multiple times after infection. The results show a startling 1000-fold range in abundance as well as strikingly different kinetics across the epitopes monitored. The tight correlation between onset of protein expression and epitope display for most antigens provides the strongest support to date that antigen presentation is largely linked to translation and not later degradation of antigens. Finally, we show a complete disconnect between the epitope abundance and immunodominance hierarchy of these eight epitopes. This study highlights the complexity of viral antigen presentation by the host and demonstrates the weakness of simple models that assume total protein levels are directly linked to epitope presentation and immunogenicity.
format	article
author	Nathan P Croft Stewart A Smith Yik Chun Wong Chor Teck Tan Nadine L Dudek Inge E A Flesch Leon C W Lin David C Tscharke Anthony W Purcell
author_facet	Nathan P Croft Stewart A Smith Yik Chun Wong Chor Teck Tan Nadine L Dudek Inge E A Flesch Leon C W Lin David C Tscharke Anthony W Purcell
author_sort	Nathan P Croft
title	Kinetics of antigen expression and epitope presentation during virus infection.
title_short	Kinetics of antigen expression and epitope presentation during virus infection.
title_full	Kinetics of antigen expression and epitope presentation during virus infection.
title_fullStr	Kinetics of antigen expression and epitope presentation during virus infection.
title_full_unstemmed	Kinetics of antigen expression and epitope presentation during virus infection.
title_sort	kinetics of antigen expression and epitope presentation during virus infection.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6a1915d21ff04a768d317385c5da648c
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Kinetics of antigen expression and epitope presentation during virus infection.

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