Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.

Cytogenetic observations, phylogenetic studies and genome analysis using high-density genetic markers have suggested a tetraploid Avena species carrying the C and D genomes (formerly C and A) to be the donor of all hexaploid oats (AACCDD). However, controversy surrounds which of the three extant CCD...

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Autores principales:	Araceli Fominaya, Yolanda Loarce, Juan M González, Esther Ferrer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a19dfac8b214c65afe7170077473199
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spelling	oai:doaj.org-article:6a19dfac8b214c65afe71700774731992021-12-02T20:16:53ZCytogenetic evidence supports Avena insularis being closely related to hexaploid oats.1932-620310.1371/journal.pone.0257100https://doaj.org/article/6a19dfac8b214c65afe71700774731992021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257100https://doaj.org/toc/1932-6203Cytogenetic observations, phylogenetic studies and genome analysis using high-density genetic markers have suggested a tetraploid Avena species carrying the C and D genomes (formerly C and A) to be the donor of all hexaploid oats (AACCDD). However, controversy surrounds which of the three extant CCDD tetraploid species-A. insularis, A. magna and A. murphyi-is most closely related to hexaploid oats. The present work describes a comparative karyotype analysis of these three CCDD tetraploid species and two hexaploid species, A. sativa and A. byzantina. This involved the use of FISH with six simple sequence repeats (SSRs) with the motifs CT, AAC, AAG, ACG, ATC and ACT, two repeated ribosomal sequences, and C genome-specific repetitive DNA. The hybridization pattern of A. insularis with oligonucleotide (AC)10 was also determined and compared with those previously published for A. sativa and A. byzantina. Significant differences in the 5S sites and SSR hybridization patterns of A. murphyi compared to the other CCDD species rule out its being directly involved in the origin of the hexaploids. In contrast, the repetitive and SSR hybridization patterns shown by the D genome chromosomes, and by most of the C genome chromosomes of A. magna and A. insularis, can be equated with the corresponding chromosomes of the hexaploids. Several chromosome hybridization signals seen for A. insularis, but not for A. magna, were shared with the hexaploid oats species, especially with A. byzantina. These diagnostic signals add weight to the idea that the extant A. insularis, or a direct ancestor of it, is the most closely related progenitor of hexaploid oats. The similarity of the chromosome hybridization patterns of the hexaploids and CCDD tetraploids was taken as being indicative of homology. A common chromosome nomenclature for CCDD species based on that of the hexaploid species is proposed.Araceli FominayaYolanda LoarceJuan M GonzálezEsther FerrerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257100 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Araceli Fominaya Yolanda Loarce Juan M González Esther Ferrer Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.
description	Cytogenetic observations, phylogenetic studies and genome analysis using high-density genetic markers have suggested a tetraploid Avena species carrying the C and D genomes (formerly C and A) to be the donor of all hexaploid oats (AACCDD). However, controversy surrounds which of the three extant CCDD tetraploid species-A. insularis, A. magna and A. murphyi-is most closely related to hexaploid oats. The present work describes a comparative karyotype analysis of these three CCDD tetraploid species and two hexaploid species, A. sativa and A. byzantina. This involved the use of FISH with six simple sequence repeats (SSRs) with the motifs CT, AAC, AAG, ACG, ATC and ACT, two repeated ribosomal sequences, and C genome-specific repetitive DNA. The hybridization pattern of A. insularis with oligonucleotide (AC)10 was also determined and compared with those previously published for A. sativa and A. byzantina. Significant differences in the 5S sites and SSR hybridization patterns of A. murphyi compared to the other CCDD species rule out its being directly involved in the origin of the hexaploids. In contrast, the repetitive and SSR hybridization patterns shown by the D genome chromosomes, and by most of the C genome chromosomes of A. magna and A. insularis, can be equated with the corresponding chromosomes of the hexaploids. Several chromosome hybridization signals seen for A. insularis, but not for A. magna, were shared with the hexaploid oats species, especially with A. byzantina. These diagnostic signals add weight to the idea that the extant A. insularis, or a direct ancestor of it, is the most closely related progenitor of hexaploid oats. The similarity of the chromosome hybridization patterns of the hexaploids and CCDD tetraploids was taken as being indicative of homology. A common chromosome nomenclature for CCDD species based on that of the hexaploid species is proposed.
format	article
author	Araceli Fominaya Yolanda Loarce Juan M González Esther Ferrer
author_facet	Araceli Fominaya Yolanda Loarce Juan M González Esther Ferrer
author_sort	Araceli Fominaya
title	Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.
title_short	Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.
title_full	Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.
title_fullStr	Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.
title_full_unstemmed	Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.
title_sort	cytogenetic evidence supports avena insularis being closely related to hexaploid oats.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/6a19dfac8b214c65afe7170077473199
work_keys_str_mv	AT aracelifominaya cytogeneticevidencesupportsavenainsularisbeingcloselyrelatedtohexaploidoats AT yolandaloarce cytogeneticevidencesupportsavenainsularisbeingcloselyrelatedtohexaploidoats AT juanmgonzalez cytogeneticevidencesupportsavenainsularisbeingcloselyrelatedtohexaploidoats AT estherferrer cytogeneticevidencesupportsavenainsularisbeingcloselyrelatedtohexaploidoats
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Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats.

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