Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway

Haitao Tao, Lei Cheng, Ruixiang Yang Orthopedic Surgery, The 3rd People’s Hospital of Qingdao, Qingdao 266041, People’s Republic of ChinaCorrespondence: Ruixiang YangOrthopedic Surgery, The 3rd People’s Hospital of Qingdao, No. 29 Yongping Road, Licang District, Qingdao...

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Autores principales:	Tao H, Cheng L, Yang R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	mir-34a proliferation apoptosis osteoarthritis pi3k/akt pathway Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/6a1bfcc87c494617b5b2232965b6ad89
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spelling	oai:doaj.org-article:6a1bfcc87c494617b5b2232965b6ad892021-12-02T09:44:18ZDownregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway1178-1998https://doaj.org/article/6a1bfcc87c494617b5b2232965b6ad892020-03-01T00:00:00Zhttps://www.dovepress.com/downregulation-of-mir-34a-promotes-proliferation-and-inhibits-apoptosi-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Haitao Tao, Lei Cheng, Ruixiang Yang Orthopedic Surgery, The 3rd People’s Hospital of Qingdao, Qingdao 266041, People’s Republic of ChinaCorrespondence: Ruixiang YangOrthopedic Surgery, The 3rd People’s Hospital of Qingdao, No. 29 Yongping Road, Licang District, Qingdao, People’s Republic of ChinaTel +86 138 6421 2816Email l7m1wzp@163.comObjective: To elucidate the expression and function of miR-34a in rat osteoarthritic cartilage cells, and further to explore its mechanism.Material and Methods: Rat model of osteoarthritis was constructed and knee joint cartilage cells were isolated in vitro. Immunocytochemical staining was used for identification. qRT-PCR was used to detect the expression of miR-34a in cartilaginous tissues and cartilage cells. Cartilage cells were divided into blank control (BC), negative control (NC), miR-34a inhibitor (34aI), osteoarthritis model (OA), osteoarthritis model + negative control (OA + NC) and osteoarthritis model + miR-34a inhibitor (OA + 34aI) groups. Cell proliferation was detected by CCK-8 and colony formation assays. Cell apoptosis was studied by flow cytometry and Western blot. PI3K/AKT-pathway-related proteins were also analyzed by Western blot. To further validate the effect of miR-34a on the PI3K/Akt pathway, the cartilage cells were divided into blank control (BC), osteoarthritis model (OA), osteoarthritis model + miR-34a inhibitor (OA + 34aI), osteoarthritis model + PI3K activator (OA + IGF-1) and osteoarthritis model + miR-34a inhibitor + PI3K inhibitor (OA + 34aI + LY) groups, the experiments above were repeated.Results: The expression of miR-34a in cartilaginous tissues and cells of osteoarthritis model was significantly higher than that in normal (p < 0.05). After silencing miR-34a gene, the cell proliferation and proteins expression of PI3K/Akt pathway were increased, while the apoptosis rate and expression of apoptosis-related proteins were decreased. Addition of PI3K activator also evidently promoted proliferation and inhibited apoptosis. The protein expression of Bax, Cleaved caspase-3 and Cleaved caspase-9 were dramatically decreased, while the ratios of p-PI3K/PI3K and p-Akt/Akt were increased in OA + IGF-1 group.Conclusion: Downregulation of miR-34a regulated proliferation and apoptosis of cartilage cells by activating PI3K/Akt pathway, providing a potential therapeutic approach for the treatment of osteoarthritis.Keywords: miR-34a, proliferation, apoptosis, osteoarthritis, PI3K/Akt pathwayTao HCheng LYang RDove Medical Pressarticlemir-34aproliferationapoptosisosteoarthritispi3k/akt pathwayGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 15, Pp 373-385 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mir-34a proliferation apoptosis osteoarthritis pi3k/akt pathway Geriatrics RC952-954.6
spellingShingle	mir-34a proliferation apoptosis osteoarthritis pi3k/akt pathway Geriatrics RC952-954.6 Tao H Cheng L Yang R Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway
description	Haitao Tao, Lei Cheng, Ruixiang Yang Orthopedic Surgery, The 3rd People’s Hospital of Qingdao, Qingdao 266041, People’s Republic of ChinaCorrespondence: Ruixiang YangOrthopedic Surgery, The 3rd People’s Hospital of Qingdao, No. 29 Yongping Road, Licang District, Qingdao, People’s Republic of ChinaTel +86 138 6421 2816Email l7m1wzp@163.comObjective: To elucidate the expression and function of miR-34a in rat osteoarthritic cartilage cells, and further to explore its mechanism.Material and Methods: Rat model of osteoarthritis was constructed and knee joint cartilage cells were isolated in vitro. Immunocytochemical staining was used for identification. qRT-PCR was used to detect the expression of miR-34a in cartilaginous tissues and cartilage cells. Cartilage cells were divided into blank control (BC), negative control (NC), miR-34a inhibitor (34aI), osteoarthritis model (OA), osteoarthritis model + negative control (OA + NC) and osteoarthritis model + miR-34a inhibitor (OA + 34aI) groups. Cell proliferation was detected by CCK-8 and colony formation assays. Cell apoptosis was studied by flow cytometry and Western blot. PI3K/AKT-pathway-related proteins were also analyzed by Western blot. To further validate the effect of miR-34a on the PI3K/Akt pathway, the cartilage cells were divided into blank control (BC), osteoarthritis model (OA), osteoarthritis model + miR-34a inhibitor (OA + 34aI), osteoarthritis model + PI3K activator (OA + IGF-1) and osteoarthritis model + miR-34a inhibitor + PI3K inhibitor (OA + 34aI + LY) groups, the experiments above were repeated.Results: The expression of miR-34a in cartilaginous tissues and cells of osteoarthritis model was significantly higher than that in normal (p < 0.05). After silencing miR-34a gene, the cell proliferation and proteins expression of PI3K/Akt pathway were increased, while the apoptosis rate and expression of apoptosis-related proteins were decreased. Addition of PI3K activator also evidently promoted proliferation and inhibited apoptosis. The protein expression of Bax, Cleaved caspase-3 and Cleaved caspase-9 were dramatically decreased, while the ratios of p-PI3K/PI3K and p-Akt/Akt were increased in OA + IGF-1 group.Conclusion: Downregulation of miR-34a regulated proliferation and apoptosis of cartilage cells by activating PI3K/Akt pathway, providing a potential therapeutic approach for the treatment of osteoarthritis.Keywords: miR-34a, proliferation, apoptosis, osteoarthritis, PI3K/Akt pathway
format	article
author	Tao H Cheng L Yang R
author_facet	Tao H Cheng L Yang R
author_sort	Tao H
title	Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway
title_short	Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway
title_full	Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway
title_fullStr	Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway
title_full_unstemmed	Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway
title_sort	downregulation of mir-34a promotes proliferation and inhibits apoptosis of rat osteoarthritic cartilage cells by activating pi3k/akt pathway
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/6a1bfcc87c494617b5b2232965b6ad89
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Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway

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