Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo

Colistin is a polymyxin antibiotic that is widely used for the treatment of multidrug resistant (MDR) Pseudomonas aeruginosa infections, as the last resort. Over the past few years, unreasonable use of antibiotics has resulted in an increase in MDR strains, including colistin-resistant P. aeruginosa...

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Autores principales:	Xiaodong Zhang, Yining Zhao, Luozhu Feng, Mengxin Xu, Yiru Ge, Lingbo Wang, Ying Zhang, Jianming Cao, Yao Sun, Qing Wu, Tieli Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	colistin resistance Pseudomonas aeruginosa synergistic effect mefloquine anti-biofilm Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6a29dc390a3343dcaa33da4eff1f08fa
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spelling	oai:doaj.org-article:6a29dc390a3343dcaa33da4eff1f08fa2021-12-01T07:26:28ZCombined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo1664-302X10.3389/fmicb.2021.790220https://doaj.org/article/6a29dc390a3343dcaa33da4eff1f08fa2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.790220/fullhttps://doaj.org/toc/1664-302XColistin is a polymyxin antibiotic that is widely used for the treatment of multidrug resistant (MDR) Pseudomonas aeruginosa infections, as the last resort. Over the past few years, unreasonable use of antibiotics has resulted in an increase in MDR strains, including colistin-resistant P. aeruginosa. The present study aimed to explore the synergistic effects of mefloquine in combination with colistin for the treatment of colistin-resistant P. aeruginosa in vivo and in vitro. The synergistic effect of the combination of mefloquine and colistin was investigated in vitro using checkerboard method, time-killing assay, biofilm formation inhibition test, and biofilm eradication test. The study also explored the synergistic effects of this combination of drugs in vivo, using a Galleria mellonella infection model. The results for checkerboard method and time killing curve indicated that mefloquine in combination with colistin showed a good antibacterial activity. Furthermore, the combination of these two drugs inhibited biofilm formation and eradicated pre-formed mature biofilms. This synergistic effect was visualized using scanning electron microscopy (SEM), wherein the results showed that the combination of mefloquine and colistin reduced biofilm formation significantly. Further, the application of this combination of drugs to in vivo infection model significantly increased the survival rate of G. mellonella larvae. Altogether, the combination of mefloquine and colistin showed a good synergistic effect in vitro and in vivo, and highlighted its potential to be used as an alternative therapy for the treatment of colistin-resistant P. aeruginosa infection.Xiaodong ZhangYining ZhaoLuozhu FengMengxin XuYiru GeLingbo WangYing ZhangJianming CaoYao SunQing WuTieli ZhouFrontiers Media S.A.articlecolistin resistancePseudomonas aeruginosasynergistic effectmefloquineanti-biofilmMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	colistin resistance Pseudomonas aeruginosa synergistic effect mefloquine anti-biofilm Microbiology QR1-502
spellingShingle	colistin resistance Pseudomonas aeruginosa synergistic effect mefloquine anti-biofilm Microbiology QR1-502 Xiaodong Zhang Yining Zhao Luozhu Feng Mengxin Xu Yiru Ge Lingbo Wang Ying Zhang Jianming Cao Yao Sun Qing Wu Tieli Zhou Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo
description	Colistin is a polymyxin antibiotic that is widely used for the treatment of multidrug resistant (MDR) Pseudomonas aeruginosa infections, as the last resort. Over the past few years, unreasonable use of antibiotics has resulted in an increase in MDR strains, including colistin-resistant P. aeruginosa. The present study aimed to explore the synergistic effects of mefloquine in combination with colistin for the treatment of colistin-resistant P. aeruginosa in vivo and in vitro. The synergistic effect of the combination of mefloquine and colistin was investigated in vitro using checkerboard method, time-killing assay, biofilm formation inhibition test, and biofilm eradication test. The study also explored the synergistic effects of this combination of drugs in vivo, using a Galleria mellonella infection model. The results for checkerboard method and time killing curve indicated that mefloquine in combination with colistin showed a good antibacterial activity. Furthermore, the combination of these two drugs inhibited biofilm formation and eradicated pre-formed mature biofilms. This synergistic effect was visualized using scanning electron microscopy (SEM), wherein the results showed that the combination of mefloquine and colistin reduced biofilm formation significantly. Further, the application of this combination of drugs to in vivo infection model significantly increased the survival rate of G. mellonella larvae. Altogether, the combination of mefloquine and colistin showed a good synergistic effect in vitro and in vivo, and highlighted its potential to be used as an alternative therapy for the treatment of colistin-resistant P. aeruginosa infection.
format	article
author	Xiaodong Zhang Yining Zhao Luozhu Feng Mengxin Xu Yiru Ge Lingbo Wang Ying Zhang Jianming Cao Yao Sun Qing Wu Tieli Zhou
author_facet	Xiaodong Zhang Yining Zhao Luozhu Feng Mengxin Xu Yiru Ge Lingbo Wang Ying Zhang Jianming Cao Yao Sun Qing Wu Tieli Zhou
author_sort	Xiaodong Zhang
title	Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo
title_short	Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo
title_full	Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo
title_fullStr	Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo
title_full_unstemmed	Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo
title_sort	combined with mefloquine, resurrect colistin active in colistin-resistant pseudomonas aeruginosa in vitro and in vivo
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6a29dc390a3343dcaa33da4eff1f08fa
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Combined With Mefloquine, Resurrect Colistin Active in Colistin-Resistant Pseudomonas aeruginosa in vitro and in vivo

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