Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination

Elena Romero-Ben,1 Juan José Cid,1 Mohyeddin Assali,1 Elisabeth Fernández-García,2 Ralf Erik Wellinger,2 Noureddine Khiar1 1Asymmetric Synthesis and Functional Nanosystems Group, Institute of Chemical Research-Universidad de Sevilla, Avda. Américo Vespuci...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Romero-Ben E, Cid JJ, Assali M, Fernández-García E, Wellinger RE, Khiar N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Single wall Carbon Nanotubes Carbohydrates Bacterial Resistance Antiadhesive Therapy Multivalency. Aryl Diazonium Salts Chemistry Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6a3127466a17461b87ed3a28e99c2781
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6a3127466a17461b87ed3a28e99c2781
record_format	dspace
spelling	oai:doaj.org-article:6a3127466a17461b87ed3a28e99c27812021-12-02T05:27:12ZSurface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination1178-2013https://doaj.org/article/6a3127466a17461b87ed3a28e99c27812019-05-01T00:00:00Zhttps://www.dovepress.com/surface-modulation-of-single-walled-carbon-nanotubes-for-selective-bac-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Elena Romero-Ben,1 Juan José Cid,1 Mohyeddin Assali,1 Elisabeth Fernández-García,2 Ralf Erik Wellinger,2 Noureddine Khiar1 1Asymmetric Synthesis and Functional Nanosystems Group, Institute of Chemical Research-Universidad de Sevilla, Avda. Américo Vespucio, 41092 Seville, Spain; 2Genome Stability Department, Andalusian Center for Molecular Biology and Regenerative Medicine Centre, Universidad de Sevilla-CSIC, Avda. Américo Vespucio, 41092 Seville, Spain Background: Bacterial resistance to antibiotics is one of the biggest challenges facing medicine today. Anti-adhesive therapy, using inhibitors of bacterial adhesion to epithelial cells, one of the first stages of infection, is a promising approximation in this area. The size, shape, number of sugar and their placement are variables that have to be taken into account in order to develop multivalent systems able to inhibit the bacterial adhesion based on sugar-lectin interaction. Materials and methods: In the present work we report a modular approach for the synthesis of water-soluble 1D-carbon nanotube-sugar nanoconstructs, with the necessary flexibility to allow an efficient sugar-lectin interaction. The method is based on the reaction of aryl diazonium salts generated in situ from aniline-substituted mannose and lactose derivatives with single wall carbon nanotubes (SWCNTs) sidewalls. Results: Two hybrid nanosystems, I-II, exposing mannose or lactose and having a tetraethylene glycol spacer between the sugar and the nanotube sidewall were rapidly assembled and adequately characterized. The sweet nano-objects were then tested for their ability to agglutinate and selectively inhibit the growth of uropathogenic Escherichia coli. These studies have shown that nanosystem I, exposing mannose on the nanotube surface is able to agglutinate and to inhibit the bacterial growth unlike nano-objects II exposing lactose.Conclusion: The results reported constitute a proof of principle in using mannose-coated 1D-carbon nanotubes as antiadhesive drugs that compete for FimH binding and prevent the uropathogenic bacteria from adhering to the urothelial surface. Keywords: single-walled carbon nanotubes, carbohydrates, bacterial resistance, antiadhesive therapy, multivalency, aryl diazonium salts chemistryRomero-Ben ECid JJAssali MFernández-García EWellinger REKhiar NDove Medical PressarticleSingle wall Carbon NanotubesCarbohydratesBacterial ResistanceAntiadhesive TherapyMultivalency. Aryl Diazonium Salts ChemistryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 3245-3263 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Single wall Carbon Nanotubes Carbohydrates Bacterial Resistance Antiadhesive Therapy Multivalency. Aryl Diazonium Salts Chemistry Medicine (General) R5-920
spellingShingle	Single wall Carbon Nanotubes Carbohydrates Bacterial Resistance Antiadhesive Therapy Multivalency. Aryl Diazonium Salts Chemistry Medicine (General) R5-920 Romero-Ben E Cid JJ Assali M Fernández-García E Wellinger RE Khiar N Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination
description	Elena Romero-Ben,1 Juan José Cid,1 Mohyeddin Assali,1 Elisabeth Fernández-García,2 Ralf Erik Wellinger,2 Noureddine Khiar1 1Asymmetric Synthesis and Functional Nanosystems Group, Institute of Chemical Research-Universidad de Sevilla, Avda. Américo Vespucio, 41092 Seville, Spain; 2Genome Stability Department, Andalusian Center for Molecular Biology and Regenerative Medicine Centre, Universidad de Sevilla-CSIC, Avda. Américo Vespucio, 41092 Seville, Spain Background: Bacterial resistance to antibiotics is one of the biggest challenges facing medicine today. Anti-adhesive therapy, using inhibitors of bacterial adhesion to epithelial cells, one of the first stages of infection, is a promising approximation in this area. The size, shape, number of sugar and their placement are variables that have to be taken into account in order to develop multivalent systems able to inhibit the bacterial adhesion based on sugar-lectin interaction. Materials and methods: In the present work we report a modular approach for the synthesis of water-soluble 1D-carbon nanotube-sugar nanoconstructs, with the necessary flexibility to allow an efficient sugar-lectin interaction. The method is based on the reaction of aryl diazonium salts generated in situ from aniline-substituted mannose and lactose derivatives with single wall carbon nanotubes (SWCNTs) sidewalls. Results: Two hybrid nanosystems, I-II, exposing mannose or lactose and having a tetraethylene glycol spacer between the sugar and the nanotube sidewall were rapidly assembled and adequately characterized. The sweet nano-objects were then tested for their ability to agglutinate and selectively inhibit the growth of uropathogenic Escherichia coli. These studies have shown that nanosystem I, exposing mannose on the nanotube surface is able to agglutinate and to inhibit the bacterial growth unlike nano-objects II exposing lactose.Conclusion: The results reported constitute a proof of principle in using mannose-coated 1D-carbon nanotubes as antiadhesive drugs that compete for FimH binding and prevent the uropathogenic bacteria from adhering to the urothelial surface. Keywords: single-walled carbon nanotubes, carbohydrates, bacterial resistance, antiadhesive therapy, multivalency, aryl diazonium salts chemistry
format	article
author	Romero-Ben E Cid JJ Assali M Fernández-García E Wellinger RE Khiar N
author_facet	Romero-Ben E Cid JJ Assali M Fernández-García E Wellinger RE Khiar N
author_sort	Romero-Ben E
title	Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination
title_short	Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination
title_full	Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination
title_fullStr	Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination
title_full_unstemmed	Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination
title_sort	surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6a3127466a17461b87ed3a28e99c2781
work_keys_str_mv	AT romerobene surfacemodulationofsinglewalledcarbonnanotubesforselectivebacterialcellagglutination AT cidjj surfacemodulationofsinglewalledcarbonnanotubesforselectivebacterialcellagglutination AT assalim surfacemodulationofsinglewalledcarbonnanotubesforselectivebacterialcellagglutination AT fernandezgarciae surfacemodulationofsinglewalledcarbonnanotubesforselectivebacterialcellagglutination AT wellingerre surfacemodulationofsinglewalledcarbonnanotubesforselectivebacterialcellagglutination AT khiarn surfacemodulationofsinglewalledcarbonnanotubesforselectivebacterialcellagglutination
_version_	1718400417567604736

Surface modulation of single-walled carbon nanotubes for selective bacterial cell agglutination

Ejemplares similares