Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.

Yongcang Zhang,1,2,* Lan Liu,2,3,* Cuiting Liang,2 Lingyu Zhou,2 Lixia Tan,2 Yonghua Zong,4 Lili Wu,5 Tonghua Liu4,5 1Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, People’s Republic of China; 2Medical College, Tibet University, Lhasa...

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Autores principales:	Zhang Y, Liu L, Liang C, Zhou L, Tan L, Zong Y, Wu L, Liu T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	expression profile noncoding rnas high-altitude hypoxia gymnadenia conopsea (l.) r. br. Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/6a3757239bc54a1c8dc9ff128397087a
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spelling	oai:doaj.org-article:6a3757239bc54a1c8dc9ff128397087a2021-12-02T11:04:58ZExpression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.1178-2021https://doaj.org/article/6a3757239bc54a1c8dc9ff128397087a2020-05-01T00:00:00Zhttps://www.dovepress.com/expression-profiles-of-long-noncoding-rnas-in-mice-with-high-altitude--peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Yongcang Zhang,1,2,* Lan Liu,2,3,* Cuiting Liang,2 Lingyu Zhou,2 Lixia Tan,2 Yonghua Zong,4 Lili Wu,5 Tonghua Liu4,5 1Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, People’s Republic of China; 2Medical College, Tibet University, Lhasa 850000, People’s Republic of China; 3West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People’s Republic of China; 4Tibet Traditional Medicine University, Lhasa 850000, People’s Republic of China; 5Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lili WuKey Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of ChinaEmail qingniao_566@163.comTonghua LiuTibet Traditional Medicine University, Lhasa 850000, People’s Republic of ChinaKey Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of China Email thliu@vip.163.comBackground: The unique geographical environment at high altitudes may cause a series of diseases, such as acute altitude reaction, cerebral edema, and pulmonary edema. Gymnadenia conopsea (L.) R. Br. has been reported to have an effect on high-altitude hypoxia. However, the molecular mechanism, especially the expression of long noncoding RNAs (lncRNAs), is not yet clear.Methods: The expression profiles of lncRNAs in high-altitude hypoxia-induced brain injury mice treated with Gymnadenia conopsea (L.) R. Br. by using a microarray method.Results: A total of 226 differentially expressed lncRNAs, 126 significantly dysregulated mRNAs and 23 differentially expressed circRNAs were detected (> 2.0-fold, p< 0.05). The expression of selected lncRNAs, mRNAs and circRNAs was validated by qRT-PCR. KEGG analysis showed that the mRNAs coexpressed with lncRNAs were involved in inflammation and hypoxia pathways, including the HIF-1, PI3K-Akt, and NF-kappa B signaling pathways. The lncRNA-TF network analysis results indicated that the lncRNAs were regulated mostly by HMGA2, SRY, GATA4, SOX5, and ZBTB16.Conclusion: This study is the first to report the expression profiles of lncRNAs, mRNAs and circRNAs in mice with high-altitude hypoxia-induced brain injury treated with Gymnadenia conopsea (L.) R. Br. and may improve the understanding of the molecular mechanism of Gymnadenia conopsea (L.) R. Br. in treating high altitude hypoxia-induced brain injury.Keywords: expression profile, noncoding RNAs, high-altitude hypoxia, Gymnadenia conopsea (L.) R. BrZhang YLiu LLiang CZhou LTan LZong YWu LLiu TDove Medical Pressarticleexpression profilenoncoding rnashigh-altitude hypoxiagymnadenia conopsea (l.) r. br.Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 1239-1248 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	expression profile noncoding rnas high-altitude hypoxia gymnadenia conopsea (l.) r. br. Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	expression profile noncoding rnas high-altitude hypoxia gymnadenia conopsea (l.) r. br. Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Zhang Y Liu L Liang C Zhou L Tan L Zong Y Wu L Liu T Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.
description	Yongcang Zhang,1,2,* Lan Liu,2,3,* Cuiting Liang,2 Lingyu Zhou,2 Lixia Tan,2 Yonghua Zong,4 Lili Wu,5 Tonghua Liu4,5 1Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, People’s Republic of China; 2Medical College, Tibet University, Lhasa 850000, People’s Republic of China; 3West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People’s Republic of China; 4Tibet Traditional Medicine University, Lhasa 850000, People’s Republic of China; 5Key Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of China*These authors contributed equally to this workCorrespondence: Lili WuKey Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of ChinaEmail qingniao_566@163.comTonghua LiuTibet Traditional Medicine University, Lhasa 850000, People’s Republic of ChinaKey Laboratory of Health Cultivation of the Ministry of Education, Beijing University of Chinese Medicine, Beijing 100029, People’s Republic of China Email thliu@vip.163.comBackground: The unique geographical environment at high altitudes may cause a series of diseases, such as acute altitude reaction, cerebral edema, and pulmonary edema. Gymnadenia conopsea (L.) R. Br. has been reported to have an effect on high-altitude hypoxia. However, the molecular mechanism, especially the expression of long noncoding RNAs (lncRNAs), is not yet clear.Methods: The expression profiles of lncRNAs in high-altitude hypoxia-induced brain injury mice treated with Gymnadenia conopsea (L.) R. Br. by using a microarray method.Results: A total of 226 differentially expressed lncRNAs, 126 significantly dysregulated mRNAs and 23 differentially expressed circRNAs were detected (> 2.0-fold, p< 0.05). The expression of selected lncRNAs, mRNAs and circRNAs was validated by qRT-PCR. KEGG analysis showed that the mRNAs coexpressed with lncRNAs were involved in inflammation and hypoxia pathways, including the HIF-1, PI3K-Akt, and NF-kappa B signaling pathways. The lncRNA-TF network analysis results indicated that the lncRNAs were regulated mostly by HMGA2, SRY, GATA4, SOX5, and ZBTB16.Conclusion: This study is the first to report the expression profiles of lncRNAs, mRNAs and circRNAs in mice with high-altitude hypoxia-induced brain injury treated with Gymnadenia conopsea (L.) R. Br. and may improve the understanding of the molecular mechanism of Gymnadenia conopsea (L.) R. Br. in treating high altitude hypoxia-induced brain injury.Keywords: expression profile, noncoding RNAs, high-altitude hypoxia, Gymnadenia conopsea (L.) R. Br
format	article
author	Zhang Y Liu L Liang C Zhou L Tan L Zong Y Wu L Liu T
author_facet	Zhang Y Liu L Liang C Zhou L Tan L Zong Y Wu L Liu T
author_sort	Zhang Y
title	Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.
title_short	Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.
title_full	Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.
title_fullStr	Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.
title_full_unstemmed	Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.
title_sort	expression profiles of long noncoding rnas in mice with high-altitude hypoxia-induced brain injury treated with gymnadenia conopsea (l.) r. br.
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/6a3757239bc54a1c8dc9ff128397087a
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Expression Profiles of Long Noncoding RNAs in Mice with High-Altitude Hypoxia-Induced Brain Injury Treated with Gymnadenia conopsea (L.) R. Br.

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