Utilization of rare codon-rich markers for screening amino acid overproducers

Current toxic analogues-based amino acid overproducer screening method cannot provide accuracy, sensitivity and high throughput simultaneously. Here, the authors use rare codon rich marker for the selection of overproducers of multiple amino acids from random mutation libraries of E. coli and C. glu...

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Autores principales: Bo Zheng, Xiaoyan Ma, Ning Wang, Tingting Ding, Liwei Guo, Xiaorong Zhang, Yu Yang, Chun Li, Yi-Xin Huo
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/6a375f7309ef48ea9131810368d1e595
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Sumario:Current toxic analogues-based amino acid overproducer screening method cannot provide accuracy, sensitivity and high throughput simultaneously. Here, the authors use rare codon rich marker for the selection of overproducers of multiple amino acids from random mutation libraries of E. coli and C. glutamicum.