Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa

Abstract Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes gener...

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Autores principales:	Xiao-Xia Li, Yun-Fei Diao, Hai-Jun Wei, Shi-Yong Wang, Xin-Yan Cao, Yu-Fei Zhang, Tong Chang, Dan-Li Li, Min Kyu Kim, Baozeng Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a39ca7282394d07a7254b24b1364367
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spelling	oai:doaj.org-article:6a39ca7282394d07a7254b24b13643672021-12-02T16:06:21ZTauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa10.1038/s41598-017-07185-w2045-2322https://doaj.org/article/6a39ca7282394d07a7254b24b13643672017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07185-whttps://doaj.org/toc/2045-2322Abstract Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes generated from these sperms was poor. Here, we demonstrated that the supplementation of tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, during embryo culture improved the developmental competency of embryos derived from in vitro matured pig oocytes injected intracytoplasmically with boar ED spermatozoa by reducing the production of reactive oxygen species, the DNA degradation and fragmentation, and the expression of apoptosis-related gene Bax and Bak, and by increasing the transcription of anti-apoptosis gene Bcl-XL and Bcl-2. Furthermore, TUDCA treatment promoted the blastocyst quality manifested by the total cell numbers and the ratio of inner cell mass. Taken together, our data suggest that evaporative drying would be a potentially useful method for the routine preservation of boar sperm in combination with further optimization of subsequently embryo culture conditions.Xiao-Xia LiYun-Fei DiaoHai-Jun WeiShi-Yong WangXin-Yan CaoYu-Fei ZhangTong ChangDan-Li LiMin Kyu KimBaozeng XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao-Xia Li Yun-Fei Diao Hai-Jun Wei Shi-Yong Wang Xin-Yan Cao Yu-Fei Zhang Tong Chang Dan-Li Li Min Kyu Kim Baozeng Xu Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa
description	Abstract Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes generated from these sperms was poor. Here, we demonstrated that the supplementation of tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, during embryo culture improved the developmental competency of embryos derived from in vitro matured pig oocytes injected intracytoplasmically with boar ED spermatozoa by reducing the production of reactive oxygen species, the DNA degradation and fragmentation, and the expression of apoptosis-related gene Bax and Bak, and by increasing the transcription of anti-apoptosis gene Bcl-XL and Bcl-2. Furthermore, TUDCA treatment promoted the blastocyst quality manifested by the total cell numbers and the ratio of inner cell mass. Taken together, our data suggest that evaporative drying would be a potentially useful method for the routine preservation of boar sperm in combination with further optimization of subsequently embryo culture conditions.
format	article
author	Xiao-Xia Li Yun-Fei Diao Hai-Jun Wei Shi-Yong Wang Xin-Yan Cao Yu-Fei Zhang Tong Chang Dan-Li Li Min Kyu Kim Baozeng Xu
author_facet	Xiao-Xia Li Yun-Fei Diao Hai-Jun Wei Shi-Yong Wang Xin-Yan Cao Yu-Fei Zhang Tong Chang Dan-Li Li Min Kyu Kim Baozeng Xu
author_sort	Xiao-Xia Li
title	Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa
title_short	Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa
title_full	Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa
title_fullStr	Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa
title_full_unstemmed	Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa
title_sort	tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6a39ca7282394d07a7254b24b1364367
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Tauroursodeoxycholic acid enhances the development of porcine embryos derived from in vitro-matured oocytes and evaporatively dried spermatozoa

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