Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam

Gitishree Das,1 Jayanta Kumar Patra,1 Nagaraj Basavegowda,2 Chethala N Vishnuprasad,3 Han-Seung Shin41Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Seoul, Gyeonggi-do 10326, Republic of Korea; 2Department of Biotechnology, Yeungnam University, Gyeongsan...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Das G, Patra JK, Basavegowda N, Vishnuprasad CN, Shin HS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Antidiabetic antibacterial antioxidant cytotoxicity silver nanoparticles Ipomoea batatas Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6a3a60de4c6a46039c413b7063627fc2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6a3a60de4c6a46039c413b7063627fc2
record_format	dspace
spelling	oai:doaj.org-article:6a3a60de4c6a46039c413b7063627fc22021-12-02T09:32:15ZComparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam1178-2013https://doaj.org/article/6a3a60de4c6a46039c413b7063627fc22019-07-01T00:00:00Zhttps://www.dovepress.com/comparative-study-on-antidiabetic-cytotoxicity-antioxidant-and-antibac-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Gitishree Das,1 Jayanta Kumar Patra,1 Nagaraj Basavegowda,2 Chethala N Vishnuprasad,3 Han-Seung Shin41Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Seoul, Gyeonggi-do 10326, Republic of Korea; 2Department of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea; 3Centre for Ayurveda Biology and Holistic Nutrition, The University of Trans-Disciplinary Health Sciences and Technology (TDU), Bengaluru 560064, India; 4Department of Food Science and Biotechnology, Dongguk University, Seoul, Gyeonggi‐do 10326, Republic of KoreaBackground: Ipomoea batatas (L.) Lam.(Ib) has high content of various beneficial nutrients which helps in improving and maintaining human health. It is well known as a functional food and also a valuable source of unique natural products. It contains various phenolic and flavonoid bioactive compounds.Methods: In this study, using the outer peel of two varieties of Ib : Korean red skin sweet potato and Korean pumpkin sweet potato, silver nanoparticles (AgNPs) were synthesized (termed Ib1-AgNps and Ib2-AgNps), respectively. Characterization of Ib1-AgNPs and Ib2-AgNPs was carried out through scanning electron microscopy, Fourier-transform infrared (FT-IR) spectroscopy, energy-dispersive X-ray analysis, X-ray powder diffraction and UV-Vis spectroscopy. Further, the bio-potential of the synthesized AgNPs was investigated by antidiabetic (α-glucosidase assay), antioxidant (free radical scavenging assays), antibacterial (disc diffusion method) and cytotoxicity assays (cell viability against HepG2 cells).Results: FT-IR spectroscopy revealed the contribution of bioactive compounds existing in Ib1 and Ib2 extracts, in the biosynthesis and equilibrium of the AgNPs. Although the Ib2-AgNPs had a higher atomic percentage of Ag in comparison with Ib1-AgNPs, in the antidiabetic assay, the inhibition percentage of α-glucosidase was higher for AgNPs of Ib1 than Ib2, at all three concentrations examined. From the cytotoxicity results, HepG2 cancer cells were more sensitive to the Ib1-AgNPs in comparison to the Ib2-AgNPs-treated HepG2 cells. The antioxidant prospective was higher in Ib2-AgNPs than Ib1-AgNPs. Moreover, the Ib2-AgNPs showed inhibitory action against all five tested pathogenic bacteria, producing an inhibition zone of 8.74–11.52 mm while Ib1-AgNPs had an inhibitory effect on four of them, with an 8.67–11.23 (mm) inhibition zone.Conclusions: Overall, the results concluded that the Ib2-AgNPs exhibited relatively higher functional activity than Ib1-AgNPs, which might be credited to the greater abundance of bioactive compounds existing in Ib2 extract that acted as reducing as well as capping agents in the synthesis of Ib2-AgNPs. Overall, the current study highlights a novel cost-effective and eco-friendly AgNPs synthesis using food waste peels with biocompatibility and could be potentially utilized in biomedical and pharmaceutical industries.Keywords: antidiabetic, antibacterial, antioxidant, cytotoxicity, silver nanoparticles, Ipomoea batatasDas GPatra JKBasavegowda NVishnuprasad CNShin HSDove Medical PressarticleAntidiabeticantibacterialantioxidantcytotoxicitysilver nanoparticlesIpomoea batatasMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4741-4754 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antidiabetic antibacterial antioxidant cytotoxicity silver nanoparticles Ipomoea batatas Medicine (General) R5-920
spellingShingle	Antidiabetic antibacterial antioxidant cytotoxicity silver nanoparticles Ipomoea batatas Medicine (General) R5-920 Das G Patra JK Basavegowda N Vishnuprasad CN Shin HS Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam
description	Gitishree Das,1 Jayanta Kumar Patra,1 Nagaraj Basavegowda,2 Chethala N Vishnuprasad,3 Han-Seung Shin41Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Seoul, Gyeonggi-do 10326, Republic of Korea; 2Department of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea; 3Centre for Ayurveda Biology and Holistic Nutrition, The University of Trans-Disciplinary Health Sciences and Technology (TDU), Bengaluru 560064, India; 4Department of Food Science and Biotechnology, Dongguk University, Seoul, Gyeonggi‐do 10326, Republic of KoreaBackground: Ipomoea batatas (L.) Lam.(Ib) has high content of various beneficial nutrients which helps in improving and maintaining human health. It is well known as a functional food and also a valuable source of unique natural products. It contains various phenolic and flavonoid bioactive compounds.Methods: In this study, using the outer peel of two varieties of Ib : Korean red skin sweet potato and Korean pumpkin sweet potato, silver nanoparticles (AgNPs) were synthesized (termed Ib1-AgNps and Ib2-AgNps), respectively. Characterization of Ib1-AgNPs and Ib2-AgNPs was carried out through scanning electron microscopy, Fourier-transform infrared (FT-IR) spectroscopy, energy-dispersive X-ray analysis, X-ray powder diffraction and UV-Vis spectroscopy. Further, the bio-potential of the synthesized AgNPs was investigated by antidiabetic (α-glucosidase assay), antioxidant (free radical scavenging assays), antibacterial (disc diffusion method) and cytotoxicity assays (cell viability against HepG2 cells).Results: FT-IR spectroscopy revealed the contribution of bioactive compounds existing in Ib1 and Ib2 extracts, in the biosynthesis and equilibrium of the AgNPs. Although the Ib2-AgNPs had a higher atomic percentage of Ag in comparison with Ib1-AgNPs, in the antidiabetic assay, the inhibition percentage of α-glucosidase was higher for AgNPs of Ib1 than Ib2, at all three concentrations examined. From the cytotoxicity results, HepG2 cancer cells were more sensitive to the Ib1-AgNPs in comparison to the Ib2-AgNPs-treated HepG2 cells. The antioxidant prospective was higher in Ib2-AgNPs than Ib1-AgNPs. Moreover, the Ib2-AgNPs showed inhibitory action against all five tested pathogenic bacteria, producing an inhibition zone of 8.74–11.52 mm while Ib1-AgNPs had an inhibitory effect on four of them, with an 8.67–11.23 (mm) inhibition zone.Conclusions: Overall, the results concluded that the Ib2-AgNPs exhibited relatively higher functional activity than Ib1-AgNPs, which might be credited to the greater abundance of bioactive compounds existing in Ib2 extract that acted as reducing as well as capping agents in the synthesis of Ib2-AgNPs. Overall, the current study highlights a novel cost-effective and eco-friendly AgNPs synthesis using food waste peels with biocompatibility and could be potentially utilized in biomedical and pharmaceutical industries.Keywords: antidiabetic, antibacterial, antioxidant, cytotoxicity, silver nanoparticles, Ipomoea batatas
format	article
author	Das G Patra JK Basavegowda N Vishnuprasad CN Shin HS
author_facet	Das G Patra JK Basavegowda N Vishnuprasad CN Shin HS
author_sort	Das G
title	Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam
title_short	Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam
title_full	Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam
title_fullStr	Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam
title_full_unstemmed	Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam
title_sort	comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of ipomoea batatas (l.) lam
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/6a3a60de4c6a46039c413b7063627fc2
work_keys_str_mv	AT dasg comparativestudyonantidiabeticcytotoxicityantioxidantandantibacterialpropertiesofbiosynthesizedsilvernanoparticlesusingouterpeelsoftwovarietiesofipomoeabatatasllam AT patrajk comparativestudyonantidiabeticcytotoxicityantioxidantandantibacterialpropertiesofbiosynthesizedsilvernanoparticlesusingouterpeelsoftwovarietiesofipomoeabatatasllam AT basavegowdan comparativestudyonantidiabeticcytotoxicityantioxidantandantibacterialpropertiesofbiosynthesizedsilvernanoparticlesusingouterpeelsoftwovarietiesofipomoeabatatasllam AT vishnuprasadcn comparativestudyonantidiabeticcytotoxicityantioxidantandantibacterialpropertiesofbiosynthesizedsilvernanoparticlesusingouterpeelsoftwovarietiesofipomoeabatatasllam AT shinhs comparativestudyonantidiabeticcytotoxicityantioxidantandantibacterialpropertiesofbiosynthesizedsilvernanoparticlesusingouterpeelsoftwovarietiesofipomoeabatatasllam
_version_	1718398070705618944

Comparative study on antidiabetic, cytotoxicity, antioxidant and antibacterial properties of biosynthesized silver nanoparticles using outer peels of two varieties of Ipomoea batatas (L.) Lam

Ejemplares similares