3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].

Microscopic analysis of tumor vasculature plays an important role in understanding the progression and malignancy of colorectal carcinoma. However, due to the geometry of blood vessels and their connections, standard microtome-based histology is limited in providing the spatial information of the va...

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Autores principales:	Yuan-An Liu, Shien-Tung Pan, Yung-Chi Hou, Ming-Yin Shen, Shih-Jung Peng, Shiue-Cheng Tang, Yuan-Chiang Chung
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a3ae7489702412e92a92c3194cf65f6
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spelling	oai:doaj.org-article:6a3ae7489702412e92a92c3194cf65f62021-11-18T08:44:05Z3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].1932-620310.1371/journal.pone.0081857https://doaj.org/article/6a3ae7489702412e92a92c3194cf65f62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324559/?tool=EBIhttps://doaj.org/toc/1932-6203Microscopic analysis of tumor vasculature plays an important role in understanding the progression and malignancy of colorectal carcinoma. However, due to the geometry of blood vessels and their connections, standard microtome-based histology is limited in providing the spatial information of the vascular network with a 3-dimensional (3-D) continuum. To facilitate 3-D tissue analysis, we prepared transparent human colorectal biopsies by optical clearing for in-depth confocal microscopy with CD34 immunohistochemistry. Full-depth colons were obtained from colectomies performed for colorectal carcinoma. Specimens were prepared away from (control) and at the tumor site. Taking advantage of the transparent specimens, we acquired anatomic information up to 200 μm in depth for qualitative and quantitative analyses of the vasculature. Examples are given to illustrate: (1) the association between the tumor microstructure and vasculature in space, including the perivascular cuffs of tumor outgrowth, and (2) the difference between the 2-D and 3-D quantitation of microvessels. We also demonstrate that the optically cleared mucosa can be retrieved after 3-D microscopy to perform the standard microtome-based histology (H&E staining and immunohistochemistry) for systematic integration of the two tissue imaging methods. Overall, we established a new tumor histological approach to integrate 3-D imaging, illustration, and quantitation of human colonic microvessels in normal and cancerous specimens. This approach has significant promise to work with the standard histology to better characterize the tumor microenvironment in colorectal carcinoma.Yuan-An LiuShien-Tung PanYung-Chi HouMing-Yin ShenShih-Jung PengShiue-Cheng TangYuan-Chiang ChungPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81857 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuan-An Liu Shien-Tung Pan Yung-Chi Hou Ming-Yin Shen Shih-Jung Peng Shiue-Cheng Tang Yuan-Chiang Chung 3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].
description	Microscopic analysis of tumor vasculature plays an important role in understanding the progression and malignancy of colorectal carcinoma. However, due to the geometry of blood vessels and their connections, standard microtome-based histology is limited in providing the spatial information of the vascular network with a 3-dimensional (3-D) continuum. To facilitate 3-D tissue analysis, we prepared transparent human colorectal biopsies by optical clearing for in-depth confocal microscopy with CD34 immunohistochemistry. Full-depth colons were obtained from colectomies performed for colorectal carcinoma. Specimens were prepared away from (control) and at the tumor site. Taking advantage of the transparent specimens, we acquired anatomic information up to 200 μm in depth for qualitative and quantitative analyses of the vasculature. Examples are given to illustrate: (1) the association between the tumor microstructure and vasculature in space, including the perivascular cuffs of tumor outgrowth, and (2) the difference between the 2-D and 3-D quantitation of microvessels. We also demonstrate that the optically cleared mucosa can be retrieved after 3-D microscopy to perform the standard microtome-based histology (H&E staining and immunohistochemistry) for systematic integration of the two tissue imaging methods. Overall, we established a new tumor histological approach to integrate 3-D imaging, illustration, and quantitation of human colonic microvessels in normal and cancerous specimens. This approach has significant promise to work with the standard histology to better characterize the tumor microenvironment in colorectal carcinoma.
format	article
author	Yuan-An Liu Shien-Tung Pan Yung-Chi Hou Ming-Yin Shen Shih-Jung Peng Shiue-Cheng Tang Yuan-Chiang Chung
author_facet	Yuan-An Liu Shien-Tung Pan Yung-Chi Hou Ming-Yin Shen Shih-Jung Peng Shiue-Cheng Tang Yuan-Chiang Chung
author_sort	Yuan-An Liu
title	3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].
title_short	3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].
title_full	3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].
title_fullStr	3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].
title_full_unstemmed	3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].
title_sort	3-d visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6a3ae7489702412e92a92c3194cf65f6
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3-D visualization and quantitation of microvessels in transparent human colorectal carcinoma [corrected].

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