Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma

Abstract Chondrosarcoma (CS) is a rare cancer, but it is the second most common primary malignant bone tumor and highly resistant to conventional chemotherapy and radiotherapy. Aberrant DNA methylation in the promoter CpG island of Wnt inhibitory factor 1 (WIF1) has been observed in different cancer...

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Autores principales:	Pei Liu, Jacson K. Shen, Francis J. Hornicek, Fuyun Liu, Zhenfeng Duan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a3b2d7f430d47659d79a8211bb8ab85
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spelling	oai:doaj.org-article:6a3b2d7f430d47659d79a8211bb8ab852021-12-02T12:30:27ZWnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma10.1038/s41598-017-01763-82045-2322https://doaj.org/article/6a3b2d7f430d47659d79a8211bb8ab852017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01763-8https://doaj.org/toc/2045-2322Abstract Chondrosarcoma (CS) is a rare cancer, but it is the second most common primary malignant bone tumor and highly resistant to conventional chemotherapy and radiotherapy. Aberrant DNA methylation in the promoter CpG island of Wnt inhibitory factor 1 (WIF1) has been observed in different cancers. However, no studies have shown the relationship between WIF1 methylation and CS. In this study, we found promoter methylated WIF1 in both CS cell lines (CS-1 and SW1353) and tumor tissues. Western blot analysis confirmed loss WIF1 expression and activation of Wnt pathway proteins (Wnt5a/b, LRP6, and Dvl2). We subsequently examined the correlation between levels of WIF1 methylation and overall survival (OS) and progression-free survival (PFS) in CS patient samples with a follow-up spanning 234 months (mean: 57.6 months). Kaplan-Meier survival curves and log-rank tests revealed that high levels of WIF1 methylation were associated with lower OS and PFS rates (p < 0.05). Multivariate Cox hazard analysis suggested that detection of high level methylation of WIF1 could be an independent prognostic factor in OS and PFS. In conclusion, we found that WIF1 is epigenetically silenced via promoter DNA methylation in CS and propose that WIF1 methylation may serve as a potential prognostic marker for patients with CS.Pei LiuJacson K. ShenFrancis J. HornicekFuyun LiuZhenfeng DuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pei Liu Jacson K. Shen Francis J. Hornicek Fuyun Liu Zhenfeng Duan Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma
description	Abstract Chondrosarcoma (CS) is a rare cancer, but it is the second most common primary malignant bone tumor and highly resistant to conventional chemotherapy and radiotherapy. Aberrant DNA methylation in the promoter CpG island of Wnt inhibitory factor 1 (WIF1) has been observed in different cancers. However, no studies have shown the relationship between WIF1 methylation and CS. In this study, we found promoter methylated WIF1 in both CS cell lines (CS-1 and SW1353) and tumor tissues. Western blot analysis confirmed loss WIF1 expression and activation of Wnt pathway proteins (Wnt5a/b, LRP6, and Dvl2). We subsequently examined the correlation between levels of WIF1 methylation and overall survival (OS) and progression-free survival (PFS) in CS patient samples with a follow-up spanning 234 months (mean: 57.6 months). Kaplan-Meier survival curves and log-rank tests revealed that high levels of WIF1 methylation were associated with lower OS and PFS rates (p < 0.05). Multivariate Cox hazard analysis suggested that detection of high level methylation of WIF1 could be an independent prognostic factor in OS and PFS. In conclusion, we found that WIF1 is epigenetically silenced via promoter DNA methylation in CS and propose that WIF1 methylation may serve as a potential prognostic marker for patients with CS.
format	article
author	Pei Liu Jacson K. Shen Francis J. Hornicek Fuyun Liu Zhenfeng Duan
author_facet	Pei Liu Jacson K. Shen Francis J. Hornicek Fuyun Liu Zhenfeng Duan
author_sort	Pei Liu
title	Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma
title_short	Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma
title_full	Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma
title_fullStr	Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma
title_full_unstemmed	Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma
title_sort	wnt inhibitory factor 1 (wif1) methylation and its association with clinical prognosis in patients with chondrosarcoma
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6a3b2d7f430d47659d79a8211bb8ab85
work_keys_str_mv	AT peiliu wntinhibitoryfactor1wif1methylationanditsassociationwithclinicalprognosisinpatientswithchondrosarcoma AT jacsonkshen wntinhibitoryfactor1wif1methylationanditsassociationwithclinicalprognosisinpatientswithchondrosarcoma AT francisjhornicek wntinhibitoryfactor1wif1methylationanditsassociationwithclinicalprognosisinpatientswithchondrosarcoma AT fuyunliu wntinhibitoryfactor1wif1methylationanditsassociationwithclinicalprognosisinpatientswithchondrosarcoma AT zhenfengduan wntinhibitoryfactor1wif1methylationanditsassociationwithclinicalprognosisinpatientswithchondrosarcoma
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Wnt inhibitory factor 1 (WIF1) methylation and its association with clinical prognosis in patients with chondrosarcoma

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