Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy

Maobin Xie,1,2,* Dejun Fan,3,* Yi Li,4 Xiaowen He,3 Xiaoming Chen,1,2 Yufeng Chen,3 Jixiang Zhu,1,2 Guibin Xu,5 Xiaojian Wu,3 Ping Lan3 1School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China; 2Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliate...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xie MB, Fan DJ, Li Y, He XW, Chen XM, Chen YF, Zhu JX, Xu GB, Wu XJ, Lan P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	supercritical silk fibroin nanoparticle natural compounds colon cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6a3e1db6ad6f48269d601564184925d5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6a3e1db6ad6f48269d601564184925d5
record_format	dspace
spelling	oai:doaj.org-article:6a3e1db6ad6f48269d601564184925d52021-12-02T03:05:06ZSupercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy1178-2013https://doaj.org/article/6a3e1db6ad6f48269d601564184925d52017-10-01T00:00:00Zhttps://www.dovepress.com/supercritical-carbon-dioxide-developed-silk-fibroin-nanoplatform-for-s-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Maobin Xie,1,2,* Dejun Fan,3,* Yi Li,4 Xiaowen He,3 Xiaoming Chen,1,2 Yufeng Chen,3 Jixiang Zhu,1,2 Guibin Xu,5 Xiaojian Wu,3 Ping Lan3 1School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China; 2Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, China;3Department of Colorectal Surgery, Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China; 4School of Materials, University of Manchester, Manchester, UK; 5Department of Urology, Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China *These authors contributed equally to this work Purpose: To deliver insoluble natural compounds into colon cancer cells in a controlled fashion.Materials and methods: Curcumin (CM)–silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO2 (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed.Results: CM-SF NPs (<100 nm) with controllable particle size were prepared by SEDS. CM-SF NPs had a time-dependent intracellular uptake ability, which led to an improved inhibition effect on colon cancer cells. Interestingly, the anticancer effect of CM-SF NPs was improved, while the side effect on normal human colon mucosal epithelial cells was reduced by a concentration of ~10 µg/mL. The anticancer mechanism involves cell-cycle arrest in the G0/G1 and G2/M phases in association with inducing apoptotic cells.Conclusion: The natural compound-loaded SF nanoplatform prepared by SEDS indicates promising colon cancer-therapy potential. Keywords: supercritical, silk fibroin, nanoparticle, natural compounds, colon cancerXie MBFan DJLi YHe XWChen XMChen YFZhu JXXu GBWu XJLan PDove Medical Pressarticlesupercriticalsilk fibroinnanoparticlenatural compoundscolon cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7751-7761 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	supercritical silk fibroin nanoparticle natural compounds colon cancer Medicine (General) R5-920
spellingShingle	supercritical silk fibroin nanoparticle natural compounds colon cancer Medicine (General) R5-920 Xie MB Fan DJ Li Y He XW Chen XM Chen YF Zhu JX Xu GB Wu XJ Lan P Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
description	Maobin Xie,1,2,* Dejun Fan,3,* Yi Li,4 Xiaowen He,3 Xiaoming Chen,1,2 Yufeng Chen,3 Jixiang Zhu,1,2 Guibin Xu,5 Xiaojian Wu,3 Ping Lan3 1School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China; 2Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, China;3Department of Colorectal Surgery, Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China; 4School of Materials, University of Manchester, Manchester, UK; 5Department of Urology, Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China *These authors contributed equally to this work Purpose: To deliver insoluble natural compounds into colon cancer cells in a controlled fashion.Materials and methods: Curcumin (CM)–silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO2 (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed.Results: CM-SF NPs (<100 nm) with controllable particle size were prepared by SEDS. CM-SF NPs had a time-dependent intracellular uptake ability, which led to an improved inhibition effect on colon cancer cells. Interestingly, the anticancer effect of CM-SF NPs was improved, while the side effect on normal human colon mucosal epithelial cells was reduced by a concentration of ~10 µg/mL. The anticancer mechanism involves cell-cycle arrest in the G0/G1 and G2/M phases in association with inducing apoptotic cells.Conclusion: The natural compound-loaded SF nanoplatform prepared by SEDS indicates promising colon cancer-therapy potential. Keywords: supercritical, silk fibroin, nanoparticle, natural compounds, colon cancer
format	article
author	Xie MB Fan DJ Li Y He XW Chen XM Chen YF Zhu JX Xu GB Wu XJ Lan P
author_facet	Xie MB Fan DJ Li Y He XW Chen XM Chen YF Zhu JX Xu GB Wu XJ Lan P
author_sort	Xie MB
title	Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
title_short	Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
title_full	Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
title_fullStr	Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
title_full_unstemmed	Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
title_sort	supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/6a3e1db6ad6f48269d601564184925d5
work_keys_str_mv	AT xiemb supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT fandj supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT liy supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT hexw supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT chenxm supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT chenyf supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT zhujx supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT xugb supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT wuxj supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy AT lanp supercriticalcarbondioxidedevelopedsilkfibroinnanoplatformforsmartcoloncancertherapy
_version_	1718401989251956736

Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy

Ejemplares similares