Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa

Katarzyna Niemirowicz,1,2 Izabela Swiecicka,3 Agnieszka Z Wilczewska,4 Iwona Misztalewska,4 Beata Kalska-Szostko,4 Kamil Bienias,2 Robert Bucki,1,5,6 Halina Car21Department of Microbiological and Nanobiomedical Engineering, 2Department of Experimental Pharmacology, Medical University of Bialystok,...

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Autores principales:	Niemirowicz K, Swiecicka I, Wilczewska AZ, Misztalewska I, Kalska-Szostko B, Bienias K, Bucki R, Car H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6a3e73504f6744f5a64c080a4b51c559
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spelling	oai:doaj.org-article:6a3e73504f6744f5a64c080a4b51c5592021-12-02T00:27:13ZGold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa1178-2013https://doaj.org/article/6a3e73504f6744f5a64c080a4b51c5592014-05-01T00:00:00Zhttp://www.dovepress.com/gold-functionalized-magnetic-nanoparticles-restrict-growth-of-pseudomo-a16754https://doaj.org/toc/1178-2013 Katarzyna Niemirowicz,1,2 Izabela Swiecicka,3 Agnieszka Z Wilczewska,4 Iwona Misztalewska,4 Beata Kalska-Szostko,4 Kamil Bienias,2 Robert Bucki,1,5,6 Halina Car21Department of Microbiological and Nanobiomedical Engineering, 2Department of Experimental Pharmacology, Medical University of Bialystok, 3Department of Microbiology, 4Institute of Chemistry, University of Bialystok, Bialystok, 5Faculty of Health Sciences, Jan Kochanowski University, Kielce, Poland; 6Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USAAbstract: Superparamagnetic iron oxide nanoparticles (SPIONs) and their derivatives (aminosilane and gold-coated) have been widely investigated in numerous medical applications, including their potential to act as antibacterial drug carriers that may penetrate into bacteria cells and biofilm mass. Pseudomonas aeruginosa is a frequent cause of infection in hospitalized patients, and significant numbers of currently isolated clinical strains are resistant to standard antibiotic therapy. Here we describe the impact of three types of SPIONs on the growth of P. aeruginosa during long-term bacterial culture. Their size, structure, and physicochemical properties were determined using transmission electron microscopy, X-ray diffraction analysis, and Fourier transform infrared spectroscopy. We observed significant inhibition of P. aeruginosa growth in bacterial cultures continued over 96 hours in the presence of gold-functionalized nanoparticles (Fe3O4@Au). At the 48-hour time point, growth of P. aeruginosa, as assessed by the number of colonies grown from treated samples, showed the highest inhibition (decreased by 40%). These data provide strong evidence that Fe3O4@Au can dramatically reduce growth of P. aeruginosa and provide a platform for further study of the antibacterial activity of this nanomaterial.Keywords: antibacterial activity, Pseudomonas aeruginosa, superparamagnetic nanoparticles, iron oxides, gold-coated nanoparticlesNiemirowicz KSwiecicka IWilczewska AZMisztalewska IKalska-Szostko BBienias KBucki RCar HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2217-2224 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Niemirowicz K Swiecicka I Wilczewska AZ Misztalewska I Kalska-Szostko B Bienias K Bucki R Car H Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa
description	Katarzyna Niemirowicz,1,2 Izabela Swiecicka,3 Agnieszka Z Wilczewska,4 Iwona Misztalewska,4 Beata Kalska-Szostko,4 Kamil Bienias,2 Robert Bucki,1,5,6 Halina Car21Department of Microbiological and Nanobiomedical Engineering, 2Department of Experimental Pharmacology, Medical University of Bialystok, 3Department of Microbiology, 4Institute of Chemistry, University of Bialystok, Bialystok, 5Faculty of Health Sciences, Jan Kochanowski University, Kielce, Poland; 6Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, PA, USAAbstract: Superparamagnetic iron oxide nanoparticles (SPIONs) and their derivatives (aminosilane and gold-coated) have been widely investigated in numerous medical applications, including their potential to act as antibacterial drug carriers that may penetrate into bacteria cells and biofilm mass. Pseudomonas aeruginosa is a frequent cause of infection in hospitalized patients, and significant numbers of currently isolated clinical strains are resistant to standard antibiotic therapy. Here we describe the impact of three types of SPIONs on the growth of P. aeruginosa during long-term bacterial culture. Their size, structure, and physicochemical properties were determined using transmission electron microscopy, X-ray diffraction analysis, and Fourier transform infrared spectroscopy. We observed significant inhibition of P. aeruginosa growth in bacterial cultures continued over 96 hours in the presence of gold-functionalized nanoparticles (Fe3O4@Au). At the 48-hour time point, growth of P. aeruginosa, as assessed by the number of colonies grown from treated samples, showed the highest inhibition (decreased by 40%). These data provide strong evidence that Fe3O4@Au can dramatically reduce growth of P. aeruginosa and provide a platform for further study of the antibacterial activity of this nanomaterial.Keywords: antibacterial activity, Pseudomonas aeruginosa, superparamagnetic nanoparticles, iron oxides, gold-coated nanoparticles
format	article
author	Niemirowicz K Swiecicka I Wilczewska AZ Misztalewska I Kalska-Szostko B Bienias K Bucki R Car H
author_facet	Niemirowicz K Swiecicka I Wilczewska AZ Misztalewska I Kalska-Szostko B Bienias K Bucki R Car H
author_sort	Niemirowicz K
title	Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa
title_short	Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa
title_full	Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa
title_fullStr	Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa
title_full_unstemmed	Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa
title_sort	gold-functionalized magnetic nanoparticles restrict growth of pseudomonas aeruginosa
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/6a3e73504f6744f5a64c080a4b51c559
work_keys_str_mv	AT niemirowiczk goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa AT swiecickai goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa AT wilczewskaaz goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa AT misztalewskai goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa AT kalskaszostkob goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa AT bieniask goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa AT buckir goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa AT carh goldfunctionalizedmagneticnanoparticlesrestrictgrowthofpseudomonasaeruginosa
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Gold-functionalized magnetic nanoparticles restrict growth of Pseudomonas aeruginosa

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