MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.

<h4>Background</h4>MicroRNA (miRNA) expression is broadly altered in cancer, but few studies have investigated miRNA deregulation in oral squamous cell carcinoma (OSCC). Epigenetic mechanisms are involved in the regulation of >30 miRNA genes in a range of tissues, and we aimed to inve...

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Autores principales: Erik D Wiklund, Shan Gao, Toby Hulf, Tennille Sibbritt, Shalima Nair, Daniela Elena Costea, Sune B Villadsen, Vivi Bakholdt, Jesper B Bramsen, Jens A Sørensen, Annelise Krogdahl, Susan J Clark, Jørgen Kjems
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:6a4353f64720418e930cb64f218f22e92021-11-18T07:33:44ZMicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.1932-620310.1371/journal.pone.0027840https://doaj.org/article/6a4353f64720418e930cb64f218f22e92011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22132151/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNA (miRNA) expression is broadly altered in cancer, but few studies have investigated miRNA deregulation in oral squamous cell carcinoma (OSCC). Epigenetic mechanisms are involved in the regulation of >30 miRNA genes in a range of tissues, and we aimed to investigate this further in OSCC.<h4>Methods</h4>TaqMan® qRT-PCR arrays and individual assays were used to profile miRNA expression in a panel of 25 tumors with matched adjacent tissues from patients with OSCC, and 8 control paired oral stroma and epithelium from healthy volunteers. Associated DNA methylation changes of candidate epigenetically deregulated miRNA genes were measured in the same samples using the MassArray® mass spectrometry platform. MiRNA expression and DNA methylation changes were also investigated in FACS sorted CD44(high) oral cancer stem cells from primary tumor samples (CSCs), and in oral rinse and saliva from 15 OSCC patients and 7 healthy volunteers.<h4>Results</h4>MiRNA expression patterns were consistent in healthy oral epithelium and stroma, but broadly altered in both tumor and adjacent tissue from OSCC patients. MiR-375 is repressed and miR-127 activated in OSCC, and we confirm previous reports of miR-137 hypermethylation in oral cancer. The miR-200 s/miR-205 were epigenetically activated in tumors vs normal tissues, but repressed in the absence of DNA hypermethylation specifically in CD44(high) oral CSCs. Aberrant miR-375 and miR-200a expression and miR-200c-141 methylation could be detected in and distinguish OSCC patient oral rinse and saliva from healthy volunteers, suggesting a potential clinical application for OSCC specific miRNA signatures in oral fluids.<h4>Conclusions</h4>MiRNA expression and DNA methylation changes are a common event in OSCC, and we suggest miR-375, miR-127, miR-137, the miR-200 family and miR-205 as promising candidates for future investigations. Although overall activated in OSCC, miR-200/miR-205 suppression in oral CSCs indicate that cell specific silencing of these miRNAs may drive tumor expansion and progression.Erik D WiklundShan GaoToby HulfTennille SibbrittShalima NairDaniela Elena CosteaSune B VilladsenVivi BakholdtJesper B BramsenJens A SørensenAnnelise KrogdahlSusan J ClarkJørgen KjemsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 11, p e27840 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Erik D Wiklund
Shan Gao
Toby Hulf
Tennille Sibbritt
Shalima Nair
Daniela Elena Costea
Sune B Villadsen
Vivi Bakholdt
Jesper B Bramsen
Jens A Sørensen
Annelise Krogdahl
Susan J Clark
Jørgen Kjems
MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.
description <h4>Background</h4>MicroRNA (miRNA) expression is broadly altered in cancer, but few studies have investigated miRNA deregulation in oral squamous cell carcinoma (OSCC). Epigenetic mechanisms are involved in the regulation of >30 miRNA genes in a range of tissues, and we aimed to investigate this further in OSCC.<h4>Methods</h4>TaqMan® qRT-PCR arrays and individual assays were used to profile miRNA expression in a panel of 25 tumors with matched adjacent tissues from patients with OSCC, and 8 control paired oral stroma and epithelium from healthy volunteers. Associated DNA methylation changes of candidate epigenetically deregulated miRNA genes were measured in the same samples using the MassArray® mass spectrometry platform. MiRNA expression and DNA methylation changes were also investigated in FACS sorted CD44(high) oral cancer stem cells from primary tumor samples (CSCs), and in oral rinse and saliva from 15 OSCC patients and 7 healthy volunteers.<h4>Results</h4>MiRNA expression patterns were consistent in healthy oral epithelium and stroma, but broadly altered in both tumor and adjacent tissue from OSCC patients. MiR-375 is repressed and miR-127 activated in OSCC, and we confirm previous reports of miR-137 hypermethylation in oral cancer. The miR-200 s/miR-205 were epigenetically activated in tumors vs normal tissues, but repressed in the absence of DNA hypermethylation specifically in CD44(high) oral CSCs. Aberrant miR-375 and miR-200a expression and miR-200c-141 methylation could be detected in and distinguish OSCC patient oral rinse and saliva from healthy volunteers, suggesting a potential clinical application for OSCC specific miRNA signatures in oral fluids.<h4>Conclusions</h4>MiRNA expression and DNA methylation changes are a common event in OSCC, and we suggest miR-375, miR-127, miR-137, the miR-200 family and miR-205 as promising candidates for future investigations. Although overall activated in OSCC, miR-200/miR-205 suppression in oral CSCs indicate that cell specific silencing of these miRNAs may drive tumor expansion and progression.
format article
author Erik D Wiklund
Shan Gao
Toby Hulf
Tennille Sibbritt
Shalima Nair
Daniela Elena Costea
Sune B Villadsen
Vivi Bakholdt
Jesper B Bramsen
Jens A Sørensen
Annelise Krogdahl
Susan J Clark
Jørgen Kjems
author_facet Erik D Wiklund
Shan Gao
Toby Hulf
Tennille Sibbritt
Shalima Nair
Daniela Elena Costea
Sune B Villadsen
Vivi Bakholdt
Jesper B Bramsen
Jens A Sørensen
Annelise Krogdahl
Susan J Clark
Jørgen Kjems
author_sort Erik D Wiklund
title MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.
title_short MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.
title_full MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.
title_fullStr MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.
title_full_unstemmed MicroRNA alterations and associated aberrant DNA methylation patterns across multiple sample types in oral squamous cell carcinoma.
title_sort microrna alterations and associated aberrant dna methylation patterns across multiple sample types in oral squamous cell carcinoma.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/6a4353f64720418e930cb64f218f22e9
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