Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress.
Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or B. licheniformis. In this study the response of B. pumilus to oxidative stress was investigated during a treatment with high concentrations of hydrogen...
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2014
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oai:doaj.org-article:6a4c1c5c58a948b38217340027a30d2f2021-11-18T08:37:16ZBacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress.1932-620310.1371/journal.pone.0085625https://doaj.org/article/6a4c1c5c58a948b38217340027a30d2f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24465625/?tool=EBIhttps://doaj.org/toc/1932-6203Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or B. licheniformis. In this study the response of B. pumilus to oxidative stress was investigated during a treatment with high concentrations of hydrogen peroxide at the proteome, transcriptome and metabolome level. Genes/proteins belonging to regulons, which are known to have important functions in the oxidative stress response of other organisms, were found to be upregulated, such as the Fur, Spx, SOS or CtsR regulon. Strikingly, parts of the fundamental PerR regulon responding to peroxide stress in B. subtilis are not encoded in the B. pumilus genome. Thus, B. pumilus misses the catalase KatA, the DNA-protection protein MrgA or the alkyl hydroperoxide reductase AhpCF. Data of this study suggests that the catalase KatX2 takes over the function of the missing KatA in the oxidative stress response of B. pumilus. The genome-wide expression analysis revealed an induction of bacillithiol (Cys-GlcN-malate, BSH) relevant genes. An analysis of the intracellular metabolites detected high intracellular levels of this protective metabolite, which indicates the importance of bacillithiol in the peroxide stress resistance of B. pumilus.Stefan HandtkeRebecca SchroeterBritta JürgenKaren MethlingRabea SchlüterDirk AlbrechtSacha A F T van HijumJohannes BongaertsKarl-Heinz MaurerMichael LalkThomas SchwederMichael HeckerBirgit VoigtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e85625 (2014) |
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| topic |
Medicine R Science Q |
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Medicine R Science Q Stefan Handtke Rebecca Schroeter Britta Jürgen Karen Methling Rabea Schlüter Dirk Albrecht Sacha A F T van Hijum Johannes Bongaerts Karl-Heinz Maurer Michael Lalk Thomas Schweder Michael Hecker Birgit Voigt Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. |
| description |
Bacillus pumilus is characterized by a higher oxidative stress resistance than other comparable industrially relevant Bacilli such as B. subtilis or B. licheniformis. In this study the response of B. pumilus to oxidative stress was investigated during a treatment with high concentrations of hydrogen peroxide at the proteome, transcriptome and metabolome level. Genes/proteins belonging to regulons, which are known to have important functions in the oxidative stress response of other organisms, were found to be upregulated, such as the Fur, Spx, SOS or CtsR regulon. Strikingly, parts of the fundamental PerR regulon responding to peroxide stress in B. subtilis are not encoded in the B. pumilus genome. Thus, B. pumilus misses the catalase KatA, the DNA-protection protein MrgA or the alkyl hydroperoxide reductase AhpCF. Data of this study suggests that the catalase KatX2 takes over the function of the missing KatA in the oxidative stress response of B. pumilus. The genome-wide expression analysis revealed an induction of bacillithiol (Cys-GlcN-malate, BSH) relevant genes. An analysis of the intracellular metabolites detected high intracellular levels of this protective metabolite, which indicates the importance of bacillithiol in the peroxide stress resistance of B. pumilus. |
| format |
article |
| author |
Stefan Handtke Rebecca Schroeter Britta Jürgen Karen Methling Rabea Schlüter Dirk Albrecht Sacha A F T van Hijum Johannes Bongaerts Karl-Heinz Maurer Michael Lalk Thomas Schweder Michael Hecker Birgit Voigt |
| author_facet |
Stefan Handtke Rebecca Schroeter Britta Jürgen Karen Methling Rabea Schlüter Dirk Albrecht Sacha A F T van Hijum Johannes Bongaerts Karl-Heinz Maurer Michael Lalk Thomas Schweder Michael Hecker Birgit Voigt |
| author_sort |
Stefan Handtke |
| title |
Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. |
| title_short |
Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. |
| title_full |
Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. |
| title_fullStr |
Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. |
| title_full_unstemmed |
Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. |
| title_sort |
bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/6a4c1c5c58a948b38217340027a30d2f |
| work_keys_str_mv |
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