Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid

Surface functionalization of dental implant surfaces has been a developing field in biomaterial research. This study aimed to obtain self-assembled monolayers (SAMs) using carboxyethylphosphonic acid on the surface of titanium (Ti) screws, and assessed the surface characteristics, biomechanical, and...

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Autores principales:	Ainhoa Aresti, Javier Aragoneses, Nansi López-Valverde, Ana Suárez, Juan Manuel Aragoneses
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	surface biofunctionalization phosphonic acid surface characteristics scanning electron microscopy oral implantology fibroblasts Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6a532691a2b6421587777ede305ab900
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spelling	oai:doaj.org-article:6a532691a2b6421587777ede305ab9002021-11-25T16:50:31ZEffectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid10.3390/biomedicines91116632227-9059https://doaj.org/article/6a532691a2b6421587777ede305ab9002021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1663https://doaj.org/toc/2227-9059Surface functionalization of dental implant surfaces has been a developing field in biomaterial research. This study aimed to obtain self-assembled monolayers (SAMs) using carboxyethylphosphonic acid on the surface of titanium (Ti) screws, and assessed the surface characteristics, biomechanical, and cellular behavior on the obtained specimens. This study had three groups, i.e., a control (untreated screws), a test group treated with phosphonic acid, and a third group with treated acid and bone morphogenetic protein (BMP-2) for in vitro analysis of cell lines. The assessed parameters included surface wettability, surface characteristics using scanning electron microscopy (SEM), protein immobilization, and cellular behavior of fibroblasts and mesenchymal stem cells of adipose tissue (MSCat cells). For surface wettability, a Welch test was performed to compare the contact angles between control (67 ± 1.83) and test (18.84 ± 0.72) groups, and a difference was observed in the mean measurements, but was not statistically significant. The SEM analysis showed significant surface roughness on the test screws and the cellular behavior of fibroblasts, and MSCat cells were significantly improved in this group, with fibroblasts having a polygonal shape with numerous vesicles and MSCat cells stable and uniformly coating the test Ti surface. Surface biofunctionalization of Ti surfaces with phosphonic acid showed promising results in this study, but remains to be clinically validated for its applications.Ainhoa ArestiJavier AragonesesNansi López-ValverdeAna SuárezJuan Manuel AragonesesMDPI AGarticlesurface biofunctionalizationphosphonic acidsurface characteristicsscanning electron microscopyoral implantologyfibroblastsBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1663, p 1663 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	surface biofunctionalization phosphonic acid surface characteristics scanning electron microscopy oral implantology fibroblasts Biology (General) QH301-705.5
spellingShingle	surface biofunctionalization phosphonic acid surface characteristics scanning electron microscopy oral implantology fibroblasts Biology (General) QH301-705.5 Ainhoa Aresti Javier Aragoneses Nansi López-Valverde Ana Suárez Juan Manuel Aragoneses Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid
description	Surface functionalization of dental implant surfaces has been a developing field in biomaterial research. This study aimed to obtain self-assembled monolayers (SAMs) using carboxyethylphosphonic acid on the surface of titanium (Ti) screws, and assessed the surface characteristics, biomechanical, and cellular behavior on the obtained specimens. This study had three groups, i.e., a control (untreated screws), a test group treated with phosphonic acid, and a third group with treated acid and bone morphogenetic protein (BMP-2) for in vitro analysis of cell lines. The assessed parameters included surface wettability, surface characteristics using scanning electron microscopy (SEM), protein immobilization, and cellular behavior of fibroblasts and mesenchymal stem cells of adipose tissue (MSCat cells). For surface wettability, a Welch test was performed to compare the contact angles between control (67 ± 1.83) and test (18.84 ± 0.72) groups, and a difference was observed in the mean measurements, but was not statistically significant. The SEM analysis showed significant surface roughness on the test screws and the cellular behavior of fibroblasts, and MSCat cells were significantly improved in this group, with fibroblasts having a polygonal shape with numerous vesicles and MSCat cells stable and uniformly coating the test Ti surface. Surface biofunctionalization of Ti surfaces with phosphonic acid showed promising results in this study, but remains to be clinically validated for its applications.
format	article
author	Ainhoa Aresti Javier Aragoneses Nansi López-Valverde Ana Suárez Juan Manuel Aragoneses
author_facet	Ainhoa Aresti Javier Aragoneses Nansi López-Valverde Ana Suárez Juan Manuel Aragoneses
author_sort	Ainhoa Aresti
title	Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid
title_short	Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid
title_full	Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid
title_fullStr	Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid
title_full_unstemmed	Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid
title_sort	effectiveness of biofunctionalization of titanium surfaces with phosphonic acid
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6a532691a2b6421587777ede305ab900
work_keys_str_mv	AT ainhoaaresti effectivenessofbiofunctionalizationoftitaniumsurfaceswithphosphonicacid AT javieraragoneses effectivenessofbiofunctionalizationoftitaniumsurfaceswithphosphonicacid AT nansilopezvalverde effectivenessofbiofunctionalizationoftitaniumsurfaceswithphosphonicacid AT anasuarez effectivenessofbiofunctionalizationoftitaniumsurfaceswithphosphonicacid AT juanmanuelaragoneses effectivenessofbiofunctionalizationoftitaniumsurfaceswithphosphonicacid
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Effectiveness of Biofunctionalization of Titanium Surfaces with Phosphonic Acid

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