Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice

To enable hearing, the sensory hair cell contains specialized subcellular structures at its apical region, including the actin-rich cuticular plate and circumferential band. ACF7 (actin crosslinking family protein 7), encoded by the gene Macf1 (microtubule and actin crosslinking factor 1), is a larg...

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Autores principales:	Benjamin L. Gilbert, Shaoyuan Zhu, Ahlam Salameh, Shenyu Sun, Kumar N. Alagramam, Brian M. McDermott
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	hair cell hearing MACF1 microtubule-actin crosslinking factor 1 ACF7 ABR (auditory brainstem response) Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6a5a2745ec184a14a2b384f163625fbc
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spelling	oai:doaj.org-article:6a5a2745ec184a14a2b384f163625fbc2021-12-01T19:44:04ZActin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice2296-634X10.3389/fcell.2021.709442https://doaj.org/article/6a5a2745ec184a14a2b384f163625fbc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.709442/fullhttps://doaj.org/toc/2296-634XTo enable hearing, the sensory hair cell contains specialized subcellular structures at its apical region, including the actin-rich cuticular plate and circumferential band. ACF7 (actin crosslinking family protein 7), encoded by the gene Macf1 (microtubule and actin crosslinking factor 1), is a large cytoskeletal crosslinking protein that interacts with microtubules and filamentous actin to shape cells. ACF7 localizes to the cuticular plate and the circumferential band in the hair cells of vertebrates. The compelling expression pattern of ACF7 in hair cells, combined with conserved roles of this protein in the cytoskeleton of various cell types in invertebrates and vertebrates, led to the hypothesis that ACF7 performs a key function in the subcellular architecture of hair cells. To test the hypothesis, we conditionally target Macf1 in the inner ears of mice. Surprisingly, our data show that in young, but mature, conditional knockout mice cochlear hair cell survival, planar cell polarity, organization of the hair cells within the organ of Corti, and capacity to hear are not significantly impacted. Overall, these results fail to support the hypothesis that ACF7 is an essential hair cell protein in young mice, and the purpose of ACF7 expression in the hair cell remains to be understood.Benjamin L. GilbertBenjamin L. GilbertShaoyuan ZhuShaoyuan ZhuAhlam SalamehShenyu SunShenyu SunKumar N. AlagramamBrian M. McDermottBrian M. McDermottBrian M. McDermottBrian M. McDermottFrontiers Media S.A.articlehair cellhearingMACF1microtubule-actin crosslinking factor 1ACF7ABR (auditory brainstem response)Biology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hair cell hearing MACF1 microtubule-actin crosslinking factor 1 ACF7 ABR (auditory brainstem response) Biology (General) QH301-705.5
spellingShingle	hair cell hearing MACF1 microtubule-actin crosslinking factor 1 ACF7 ABR (auditory brainstem response) Biology (General) QH301-705.5 Benjamin L. Gilbert Benjamin L. Gilbert Shaoyuan Zhu Shaoyuan Zhu Ahlam Salameh Shenyu Sun Shenyu Sun Kumar N. Alagramam Brian M. McDermott Brian M. McDermott Brian M. McDermott Brian M. McDermott Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice
description	To enable hearing, the sensory hair cell contains specialized subcellular structures at its apical region, including the actin-rich cuticular plate and circumferential band. ACF7 (actin crosslinking family protein 7), encoded by the gene Macf1 (microtubule and actin crosslinking factor 1), is a large cytoskeletal crosslinking protein that interacts with microtubules and filamentous actin to shape cells. ACF7 localizes to the cuticular plate and the circumferential band in the hair cells of vertebrates. The compelling expression pattern of ACF7 in hair cells, combined with conserved roles of this protein in the cytoskeleton of various cell types in invertebrates and vertebrates, led to the hypothesis that ACF7 performs a key function in the subcellular architecture of hair cells. To test the hypothesis, we conditionally target Macf1 in the inner ears of mice. Surprisingly, our data show that in young, but mature, conditional knockout mice cochlear hair cell survival, planar cell polarity, organization of the hair cells within the organ of Corti, and capacity to hear are not significantly impacted. Overall, these results fail to support the hypothesis that ACF7 is an essential hair cell protein in young mice, and the purpose of ACF7 expression in the hair cell remains to be understood.
format	article
author	Benjamin L. Gilbert Benjamin L. Gilbert Shaoyuan Zhu Shaoyuan Zhu Ahlam Salameh Shenyu Sun Shenyu Sun Kumar N. Alagramam Brian M. McDermott Brian M. McDermott Brian M. McDermott Brian M. McDermott
author_facet	Benjamin L. Gilbert Benjamin L. Gilbert Shaoyuan Zhu Shaoyuan Zhu Ahlam Salameh Shenyu Sun Shenyu Sun Kumar N. Alagramam Brian M. McDermott Brian M. McDermott Brian M. McDermott Brian M. McDermott
author_sort	Benjamin L. Gilbert
title	Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice
title_short	Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice
title_full	Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice
title_fullStr	Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice
title_full_unstemmed	Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice
title_sort	actin crosslinking family protein 7 deficiency does not impair hearing in young mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6a5a2745ec184a14a2b384f163625fbc
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Actin Crosslinking Family Protein 7 Deficiency Does Not Impair Hearing in Young Mice

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