Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo

Sheng-Hua Chu,1 Dong-Fu Feng,1 Yan-Bin Ma,1 Zhi-Qiang Li21Department of Neurosurgery, No 3 People&#39;s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China; 2Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, ChinaAbstract: Hydroxya...

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Autores principales:	Chu SH, Feng DF, Ma YB, Li ZQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6a621a77eb124455bc86a9ab7b20276b
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spelling	oai:doaj.org-article:6a621a77eb124455bc86a9ab7b20276b2021-12-02T04:54:04ZHydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo1176-91141178-2013https://doaj.org/article/6a621a77eb124455bc86a9ab7b20276b2012-07-01T00:00:00Zhttp://www.dovepress.com/hydroxyapatite-nanoparticles-inhibit-the-growth-of-human-glioma-cells--a10385https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sheng-Hua Chu,1 Dong-Fu Feng,1 Yan-Bin Ma,1 Zhi-Qiang Li21Department of Neurosurgery, No 3 People&#39;s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China; 2Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, ChinaAbstract: Hydroxyapatite nanoparticles (nano-HAPs) have been reported to exhibit antitumor effects on various human cancers, but the effects of nano-HAPs on human glioma cells remain unclear. The aim of this study was to explore the inhibitory effect of nano-HAPs on the growth of human glioma U251 and SHG44 cells in vitro and in vivo. Nano-HAPs could inhibit the growth of U251 and SHG44 cells in a dose- and time-dependent manner, according to methyl thiazoletetrazolium assay and flow cytometry. Treated with 120 mg/L and 240 mg/L nano-HAPs for 48 hours, typical apoptotic morphological changes were noted under Hoechst staining and transmission electron microscopy. The tumor growth of cells was inhibited after the injection in vivo, and the related side effects significantly decreased in the nano-HAP-and-drug combination group. Because of the function of nano-HAPs, the expression of c-Met, SATB1, Ki-67, and bcl-2 protein decreased, and the expression of SLC22A18 and caspase-3 protein decreased noticeably. The findings indicate that nano-HAPs have an evident inhibitory action and induce apoptosis of human glioma cells in vitro and in vivo. In a drug combination, they can significantly reduce the adverse reaction related to the chemotherapeutic drug 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU).Keywords: glioma, hydroxyapatite nanoparticles, growth mechanismChu SHFeng DFMa YBLi ZQDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3659-3666 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chu SH Feng DF Ma YB Li ZQ Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo
description	Sheng-Hua Chu,1 Dong-Fu Feng,1 Yan-Bin Ma,1 Zhi-Qiang Li21Department of Neurosurgery, No 3 People&#39;s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China; 2Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan, ChinaAbstract: Hydroxyapatite nanoparticles (nano-HAPs) have been reported to exhibit antitumor effects on various human cancers, but the effects of nano-HAPs on human glioma cells remain unclear. The aim of this study was to explore the inhibitory effect of nano-HAPs on the growth of human glioma U251 and SHG44 cells in vitro and in vivo. Nano-HAPs could inhibit the growth of U251 and SHG44 cells in a dose- and time-dependent manner, according to methyl thiazoletetrazolium assay and flow cytometry. Treated with 120 mg/L and 240 mg/L nano-HAPs for 48 hours, typical apoptotic morphological changes were noted under Hoechst staining and transmission electron microscopy. The tumor growth of cells was inhibited after the injection in vivo, and the related side effects significantly decreased in the nano-HAP-and-drug combination group. Because of the function of nano-HAPs, the expression of c-Met, SATB1, Ki-67, and bcl-2 protein decreased, and the expression of SLC22A18 and caspase-3 protein decreased noticeably. The findings indicate that nano-HAPs have an evident inhibitory action and induce apoptosis of human glioma cells in vitro and in vivo. In a drug combination, they can significantly reduce the adverse reaction related to the chemotherapeutic drug 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU).Keywords: glioma, hydroxyapatite nanoparticles, growth mechanism
format	article
author	Chu SH Feng DF Ma YB Li ZQ
author_facet	Chu SH Feng DF Ma YB Li ZQ
author_sort	Chu SH
title	Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo
title_short	Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo
title_full	Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo
title_fullStr	Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo
title_full_unstemmed	Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo
title_sort	hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/6a621a77eb124455bc86a9ab7b20276b
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Hydroxyapatite nanoparticles inhibit the growth of human glioma cells in vitro and in vivo

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