Towards a synthetic chloroplast.

<h4>Background</h4>The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic rela...

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Autores principales:	Christina M Agapakis, Henrike Niederholtmeyer, Ramil R Noche, Tami D Lieberman, Sean G Megason, Jeffrey C Way, Pamela A Silver
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a62563310a149178d6e3a64068d820a
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spelling	oai:doaj.org-article:6a62563310a149178d6e3a64068d820a2021-11-18T06:55:27ZTowards a synthetic chloroplast.1932-620310.1371/journal.pone.0018877https://doaj.org/article/6a62563310a149178d6e3a64068d820a2011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21533097/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic relationships between different species of cells are a valuable tool for synthetic biology, where engineered pathways based on two species could take advantage of the unique abilities of each mutualistic partner.<h4>Results</h4>We explored the possibility of using the photosynthetic bacterium Synechococcus elongatus PCC 7942 as a platform for studying evolutionary dynamics and for designing two-species synthetic biological systems. We observed that the cyanobacteria were relatively harmless to eukaryotic host cells compared to Escherichia coli when injected into the embryos of zebrafish, Danio rerio, or taken up by mammalian macrophages. In addition, when engineered with invasin from Yersinia pestis and listeriolysin O from Listeria monocytogenes, S. elongatus was able to invade cultured mammalian cells and divide inside macrophages.<h4>Conclusion</h4>Our results show that it is possible to engineer photosynthetic bacteria to invade the cytoplasm of mammalian cells for further engineering and applications in synthetic biology. Engineered invasive but non-pathogenic or immunogenic photosynthetic bacteria have great potential as synthetic biological devices.Christina M AgapakisHenrike NiederholtmeyerRamil R NocheTami D LiebermanSean G MegasonJeffrey C WayPamela A SilverPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18877 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christina M Agapakis Henrike Niederholtmeyer Ramil R Noche Tami D Lieberman Sean G Megason Jeffrey C Way Pamela A Silver Towards a synthetic chloroplast.
description	<h4>Background</h4>The evolution of eukaryotic cells is widely agreed to have proceeded through a series of endosymbiotic events between larger cells and proteobacteria or cyanobacteria, leading to the formation of mitochondria or chloroplasts, respectively. Engineered endosymbiotic relationships between different species of cells are a valuable tool for synthetic biology, where engineered pathways based on two species could take advantage of the unique abilities of each mutualistic partner.<h4>Results</h4>We explored the possibility of using the photosynthetic bacterium Synechococcus elongatus PCC 7942 as a platform for studying evolutionary dynamics and for designing two-species synthetic biological systems. We observed that the cyanobacteria were relatively harmless to eukaryotic host cells compared to Escherichia coli when injected into the embryos of zebrafish, Danio rerio, or taken up by mammalian macrophages. In addition, when engineered with invasin from Yersinia pestis and listeriolysin O from Listeria monocytogenes, S. elongatus was able to invade cultured mammalian cells and divide inside macrophages.<h4>Conclusion</h4>Our results show that it is possible to engineer photosynthetic bacteria to invade the cytoplasm of mammalian cells for further engineering and applications in synthetic biology. Engineered invasive but non-pathogenic or immunogenic photosynthetic bacteria have great potential as synthetic biological devices.
format	article
author	Christina M Agapakis Henrike Niederholtmeyer Ramil R Noche Tami D Lieberman Sean G Megason Jeffrey C Way Pamela A Silver
author_facet	Christina M Agapakis Henrike Niederholtmeyer Ramil R Noche Tami D Lieberman Sean G Megason Jeffrey C Way Pamela A Silver
author_sort	Christina M Agapakis
title	Towards a synthetic chloroplast.
title_short	Towards a synthetic chloroplast.
title_full	Towards a synthetic chloroplast.
title_fullStr	Towards a synthetic chloroplast.
title_full_unstemmed	Towards a synthetic chloroplast.
title_sort	towards a synthetic chloroplast.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/6a62563310a149178d6e3a64068d820a
work_keys_str_mv	AT christinamagapakis towardsasyntheticchloroplast AT henrikeniederholtmeyer towardsasyntheticchloroplast AT ramilrnoche towardsasyntheticchloroplast AT tamidlieberman towardsasyntheticchloroplast AT seangmegason towardsasyntheticchloroplast AT jeffreycway towardsasyntheticchloroplast AT pamelaasilver towardsasyntheticchloroplast
_version_	1718424170604265472

Towards a synthetic chloroplast.

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