Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method

Pankaj Ranjan Karn,1,3 Wonkyung Cho,1,3 Hee-Jun Park,1,3 Jeong-Sook Park,3 Sung-Joo Hwang1,21Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Yeonsu-gu, Incheon, Republic of Korea; 2College of Pharmacy, Yonsei University, Yeonsu-gu, Incheon, Republic of Korea; 3College of Pharmacy, Ch...

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Autores principales:	Karn PR, Cho W, Park HJ, Park JS, Hwang SJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6a64be9fa44946b48cd7eaab4adb97cb
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spelling	oai:doaj.org-article:6a64be9fa44946b48cd7eaab4adb97cb2021-12-02T01:11:49ZCharacterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method1176-91141178-2013https://doaj.org/article/6a64be9fa44946b48cd7eaab4adb97cb2013-01-01T00:00:00Zhttp://www.dovepress.com/characterization-and-stability-studies-of-a-novel-liposomal-cyclospori-a12038https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Pankaj Ranjan Karn,1,3 Wonkyung Cho,1,3 Hee-Jun Park,1,3 Jeong-Sook Park,3 Sung-Joo Hwang1,21Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Yeonsu-gu, Incheon, Republic of Korea; 2College of Pharmacy, Yonsei University, Yeonsu-gu, Incheon, Republic of Korea; 3College of Pharmacy, Chungnam National University, Yuseong-gu, Daejeon, Republic of KoreaAbstract: A novel method to prepare cyclosporin A encapsulated liposomes was introduced using supercritical fluid of carbon dioxide (SCF-CO2) as an antisolvent. To investigate the strength of the newly developed SCF-CO2 method compared with the modified conventional Bangham method, particle size, zeta potential, and polydispersity index (PDI) of both liposomal formulations were characterized and compared. In addition, entrapment efficiency (EE) and drug loading (DL) characteristics were analyzed by reversed-phase high-performance liquid chromatography. Significantly larger particle size and PDI were revealed from the conventional method, while EE (%) and DL (%) did not exhibit any significant differences. The SCF-CO2 liposomes were found to be relatively smaller, multilamellar, and spherical with a smoother surface as determined by transmission electron microscopy. SCF-CO2 liposomes showed no significant differences in their particle size and PDI after more than 3 months, whereas conventional liposomes exhibited significant changes in their particle size. The initial yield (%), EE (%), and DL (%) of SCF-CO2 liposomes and conventional liposomes were 90.98 &plusmn; 2.94, 92.20 &plusmn; 1.36, 20.99 &plusmn; 0.84 and 90.72 &plusmn; 2.83, 90.24 &plusmn; 1.37, 20.47 &plusmn; 0.94, respectively, which changed after 14 weeks to 86.65 &plusmn; 0.30, 87.63 &plusmn; 0.72, 18.98 &plusmn; 0.22 and 75.04 &plusmn; 8.80, 84.59 &plusmn; 5.13, 15.94 &plusmn; 2.80, respectively. Therefore, the newly developed SCF-CO2 method could be a better alternative compared with the conventional method and may provide a promising approach for large-scale production of liposomes.Keywords: supercritical carbon dioxide, liposome, chemical stability, physical stabilityKarn PRCho WPark HJPark JSHwang SJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 365-377 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Karn PR Cho W Park HJ Park JS Hwang SJ Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method
description	Pankaj Ranjan Karn,1,3 Wonkyung Cho,1,3 Hee-Jun Park,1,3 Jeong-Sook Park,3 Sung-Joo Hwang1,21Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Yeonsu-gu, Incheon, Republic of Korea; 2College of Pharmacy, Yonsei University, Yeonsu-gu, Incheon, Republic of Korea; 3College of Pharmacy, Chungnam National University, Yuseong-gu, Daejeon, Republic of KoreaAbstract: A novel method to prepare cyclosporin A encapsulated liposomes was introduced using supercritical fluid of carbon dioxide (SCF-CO2) as an antisolvent. To investigate the strength of the newly developed SCF-CO2 method compared with the modified conventional Bangham method, particle size, zeta potential, and polydispersity index (PDI) of both liposomal formulations were characterized and compared. In addition, entrapment efficiency (EE) and drug loading (DL) characteristics were analyzed by reversed-phase high-performance liquid chromatography. Significantly larger particle size and PDI were revealed from the conventional method, while EE (%) and DL (%) did not exhibit any significant differences. The SCF-CO2 liposomes were found to be relatively smaller, multilamellar, and spherical with a smoother surface as determined by transmission electron microscopy. SCF-CO2 liposomes showed no significant differences in their particle size and PDI after more than 3 months, whereas conventional liposomes exhibited significant changes in their particle size. The initial yield (%), EE (%), and DL (%) of SCF-CO2 liposomes and conventional liposomes were 90.98 &plusmn; 2.94, 92.20 &plusmn; 1.36, 20.99 &plusmn; 0.84 and 90.72 &plusmn; 2.83, 90.24 &plusmn; 1.37, 20.47 &plusmn; 0.94, respectively, which changed after 14 weeks to 86.65 &plusmn; 0.30, 87.63 &plusmn; 0.72, 18.98 &plusmn; 0.22 and 75.04 &plusmn; 8.80, 84.59 &plusmn; 5.13, 15.94 &plusmn; 2.80, respectively. Therefore, the newly developed SCF-CO2 method could be a better alternative compared with the conventional method and may provide a promising approach for large-scale production of liposomes.Keywords: supercritical carbon dioxide, liposome, chemical stability, physical stability
format	article
author	Karn PR Cho W Park HJ Park JS Hwang SJ
author_facet	Karn PR Cho W Park HJ Park JS Hwang SJ
author_sort	Karn PR
title	Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method
title_short	Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method
title_full	Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method
title_fullStr	Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method
title_full_unstemmed	Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method
title_sort	characterization and stability studies of a novel liposomal cyclosporin a prepared using the supercritical fluid method: comparison with the modified conventional bangham method
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/6a64be9fa44946b48cd7eaab4adb97cb
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Characterization and stability studies of a novel liposomal cyclosporin A prepared using the supercritical fluid method: comparison with the modified conventional Bangham method

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