Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat

Abstract Dwarf bunt of wheat, which is caused by Tilletia controversa J.G. Kühn, is a soil-borne disease which may lead up to an 80% loss of yield together with degradation of the quality of the wheat flour by production of a fishy smell. In this study, high-throughput sequencing technology was empl...

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Autores principales:	Tongshuo Xu, Wenli Jiang, Dandan Qin, Taiguo Liu, Jianmin Zhang, Wanquan Chen, Li Gao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a672e54cdf54e09b1ca39f3fbb787dc
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spelling	oai:doaj.org-article:6a672e54cdf54e09b1ca39f3fbb787dc2021-12-02T15:53:58ZCharacterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat10.1038/s41598-021-85281-82045-2322https://doaj.org/article/6a672e54cdf54e09b1ca39f3fbb787dc2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85281-8https://doaj.org/toc/2045-2322Abstract Dwarf bunt of wheat, which is caused by Tilletia controversa J.G. Kühn, is a soil-borne disease which may lead up to an 80% loss of yield together with degradation of the quality of the wheat flour by production of a fishy smell. In this study, high-throughput sequencing technology was employed to characterize the microbial composition of wheat tissues (roots, spikes, first stem under the ear, and stem base) and rhizosphere soil of wheat varieties that are resistant and susceptible to T. controversa. We observed that the soil fungal community abundance and diversity were higher in resistant varieties than in susceptible varieties in both inoculated and uninoculated wheat, and the abundances of Sordariomycetes and Mortierellomycetes increased in the resistant varieties infected with T. controversa, while the abundances of Dothideomycetes and Bacteroidia increased in the susceptible varieties. Regarding the bacteria present in wheat tissues, the abundances of Chloroflexi, Bacteroidetes, Gemmatimonadetes, Verrucomicrobia and Acidobacteria in the ear and the first stem under the ear were higher than those in other tissues. Our results indicated that the abundances of Sordariomycetes, Mortierellomycetes, Leotiomycetes, Chryseobacterium and Massilia were higher in T. controversa-infected resistant varieties than in their controls, that Dothideomycetes, Bacteroidia, Nocardioides and Pseudomonas showed higher abundances in T. controversa-infected susceptible varieties, and that Curtobacterium, Exiguobacterium, Planococcus, and Pantoea may have higher abundances in both T. controversa-infected susceptible and resistant varieties than in their own controls.Tongshuo XuWenli JiangDandan QinTaiguo LiuJianmin ZhangWanquan ChenLi GaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tongshuo Xu Wenli Jiang Dandan Qin Taiguo Liu Jianmin Zhang Wanquan Chen Li Gao Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat
description	Abstract Dwarf bunt of wheat, which is caused by Tilletia controversa J.G. Kühn, is a soil-borne disease which may lead up to an 80% loss of yield together with degradation of the quality of the wheat flour by production of a fishy smell. In this study, high-throughput sequencing technology was employed to characterize the microbial composition of wheat tissues (roots, spikes, first stem under the ear, and stem base) and rhizosphere soil of wheat varieties that are resistant and susceptible to T. controversa. We observed that the soil fungal community abundance and diversity were higher in resistant varieties than in susceptible varieties in both inoculated and uninoculated wheat, and the abundances of Sordariomycetes and Mortierellomycetes increased in the resistant varieties infected with T. controversa, while the abundances of Dothideomycetes and Bacteroidia increased in the susceptible varieties. Regarding the bacteria present in wheat tissues, the abundances of Chloroflexi, Bacteroidetes, Gemmatimonadetes, Verrucomicrobia and Acidobacteria in the ear and the first stem under the ear were higher than those in other tissues. Our results indicated that the abundances of Sordariomycetes, Mortierellomycetes, Leotiomycetes, Chryseobacterium and Massilia were higher in T. controversa-infected resistant varieties than in their controls, that Dothideomycetes, Bacteroidia, Nocardioides and Pseudomonas showed higher abundances in T. controversa-infected susceptible varieties, and that Curtobacterium, Exiguobacterium, Planococcus, and Pantoea may have higher abundances in both T. controversa-infected susceptible and resistant varieties than in their own controls.
format	article
author	Tongshuo Xu Wenli Jiang Dandan Qin Taiguo Liu Jianmin Zhang Wanquan Chen Li Gao
author_facet	Tongshuo Xu Wenli Jiang Dandan Qin Taiguo Liu Jianmin Zhang Wanquan Chen Li Gao
author_sort	Tongshuo Xu
title	Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat
title_short	Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat
title_full	Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat
title_fullStr	Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat
title_full_unstemmed	Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat
title_sort	characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6a672e54cdf54e09b1ca39f3fbb787dc
work_keys_str_mv	AT tongshuoxu characterizationofthemicrobialcommunitiesinwheattissuesandrhizospheresoilcausedbydwarfbuntofwheat AT wenlijiang characterizationofthemicrobialcommunitiesinwheattissuesandrhizospheresoilcausedbydwarfbuntofwheat AT dandanqin characterizationofthemicrobialcommunitiesinwheattissuesandrhizospheresoilcausedbydwarfbuntofwheat AT taiguoliu characterizationofthemicrobialcommunitiesinwheattissuesandrhizospheresoilcausedbydwarfbuntofwheat AT jianminzhang characterizationofthemicrobialcommunitiesinwheattissuesandrhizospheresoilcausedbydwarfbuntofwheat AT wanquanchen characterizationofthemicrobialcommunitiesinwheattissuesandrhizospheresoilcausedbydwarfbuntofwheat AT ligao characterizationofthemicrobialcommunitiesinwheattissuesandrhizospheresoilcausedbydwarfbuntofwheat
_version_	1718385513803546624

Characterization of the microbial communities in wheat tissues and rhizosphere soil caused by dwarf bunt of wheat

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