Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>

Antibiotics are well known drugs which, when present above certain concentrations, are able to inhibit the growth of certain bacteria. However, a growing body of evidence shows that even when present at lower doses (subMIC, for sub-minimal inhibitory concentration), unable to inhibit or affect micro...

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Autores principales:	André Carvalho, Evelyne Krin, Chloé Korlowski, Didier Mazel, Zeynep Baharoglu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	quorum sensing aminoglycosides SOS response <i>Vibrio cholerae</i> bacterial signaling antibiotic tolerance Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/6a6a9ced4b4046c19aa8e0e33103fa17
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spelling	oai:doaj.org-article:6a6a9ced4b4046c19aa8e0e33103fa172021-11-25T17:13:04ZInterplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>10.3390/cells101132272073-4409https://doaj.org/article/6a6a9ced4b4046c19aa8e0e33103fa172021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3227https://doaj.org/toc/2073-4409Antibiotics are well known drugs which, when present above certain concentrations, are able to inhibit the growth of certain bacteria. However, a growing body of evidence shows that even when present at lower doses (subMIC, for sub-minimal inhibitory concentration), unable to inhibit or affect microbial growth, antibiotics work as signaling molecules, affect gene expression and trigger important bacterial stress responses. However, how subMIC antibiotic signaling interplays with other well-known signaling networks in bacteria (and the consequences of such interplay) is not well understood. In this work, through transcriptomic and genetic approaches, we have explored how quorum-sensing (QS) proficiency of <i>V. cholerae</i> affects this pathogen’s response to subMIC doses of the aminoglycoside tobramycin (TOB). We show that the transcriptomic signature of <i>V. cholerae</i> in response to subMIC TOB depends highly on the presence of QS master regulator HapR. In parallel, we show that subMIC doses of TOB are able to negatively interfere with the AI-2/LuxS QS network of <i>V. cholerae</i>, which seems critical for survival to aminoglycoside treatment and TOB-mediated induction of SOS response in this species. This interplay between QS and aminoglycosides suggests that targeting QS signaling may be a strategy to enhance aminoglycoside efficacy in <i>V. cholerae</i>.André CarvalhoEvelyne KrinChloé KorlowskiDidier MazelZeynep BaharogluMDPI AGarticlequorum sensingaminoglycosidesSOS response<i>Vibrio cholerae</i>bacterial signalingantibiotic toleranceBiology (General)QH301-705.5ENCells, Vol 10, Iss 3227, p 3227 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	quorum sensing aminoglycosides SOS response <i>Vibrio cholerae</i> bacterial signaling antibiotic tolerance Biology (General) QH301-705.5
spellingShingle	quorum sensing aminoglycosides SOS response <i>Vibrio cholerae</i> bacterial signaling antibiotic tolerance Biology (General) QH301-705.5 André Carvalho Evelyne Krin Chloé Korlowski Didier Mazel Zeynep Baharoglu Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>
description	Antibiotics are well known drugs which, when present above certain concentrations, are able to inhibit the growth of certain bacteria. However, a growing body of evidence shows that even when present at lower doses (subMIC, for sub-minimal inhibitory concentration), unable to inhibit or affect microbial growth, antibiotics work as signaling molecules, affect gene expression and trigger important bacterial stress responses. However, how subMIC antibiotic signaling interplays with other well-known signaling networks in bacteria (and the consequences of such interplay) is not well understood. In this work, through transcriptomic and genetic approaches, we have explored how quorum-sensing (QS) proficiency of <i>V. cholerae</i> affects this pathogen’s response to subMIC doses of the aminoglycoside tobramycin (TOB). We show that the transcriptomic signature of <i>V. cholerae</i> in response to subMIC TOB depends highly on the presence of QS master regulator HapR. In parallel, we show that subMIC doses of TOB are able to negatively interfere with the AI-2/LuxS QS network of <i>V. cholerae</i>, which seems critical for survival to aminoglycoside treatment and TOB-mediated induction of SOS response in this species. This interplay between QS and aminoglycosides suggests that targeting QS signaling may be a strategy to enhance aminoglycoside efficacy in <i>V. cholerae</i>.
format	article
author	André Carvalho Evelyne Krin Chloé Korlowski Didier Mazel Zeynep Baharoglu
author_facet	André Carvalho Evelyne Krin Chloé Korlowski Didier Mazel Zeynep Baharoglu
author_sort	André Carvalho
title	Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>
title_short	Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>
title_full	Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>
title_fullStr	Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>
title_full_unstemmed	Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>
title_sort	interplay between sublethal aminoglycosides and quorum sensing: consequences on survival in <i>v. cholerae</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6a6a9ced4b4046c19aa8e0e33103fa17
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Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in <i>V. cholerae</i>

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