Anti-Staphylococcus aureus Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo

Staphylococcus aureus is a causative agent of bovine mastitis, capable of causing significant economic losses to the dairy industry worldwide. This study focuses on obtaining single-chain fragment variables (scFvs) against the virulence factors of S. aureus and...

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Autores principales:	Lei Zhang, Xin Ye, Yan Zhang, Fengqing Wang, Fanqing Zhang, Yan Jia, Dangjin Wu, Kalbinur Tohti, Manling Cheng, Jianguo Zhu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bovine mastitis <i>Staphylococcus aureus</i> (<i>S. aureus</i>) single-chain fragment variable (scFv) bovine mammary epithelial (MAC-T) cells cytotoxicity anti-inflammatory Medicine R
Acceso en línea:	https://doaj.org/article/6a6da6ed37cc49b687b4bd52087df297
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spelling	oai:doaj.org-article:6a6da6ed37cc49b687b4bd52087df2972021-11-25T19:11:05ZAnti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo10.3390/vaccines91113002076-393Xhttps://doaj.org/article/6a6da6ed37cc49b687b4bd52087df2972021-11-01T00:00:00Zhttps://www.mdpi.com/2076-393X/9/11/1300https://doaj.org/toc/2076-393X<i>Staphylococcus aureus</i> is a causative agent of bovine mastitis, capable of causing significant economic losses to the dairy industry worldwide. This study focuses on obtaining single-chain fragment variables (scFvs) against the virulence factors of <i>S. aureus</i> and evaluates the protective effect of scFvs on bovine mammary epithelial (MAC-T) cells and mice mammary gland tissues infected by <i>S. aureus</i>. After five rounds of bio-panning, four scFvs targeting four virulence factors of <i>S. aureus</i> were obtained. The complementarity-determining regions (CDRs) of these scFvs exhibited significant diversities, especially CDR3 of the VH domain. In vitro, each of scFvs was capable of inhibiting <i>S. aureus</i> growth and reducing the damage of MAC-T cells infected by <i>S. aureus</i>. Preincubation of MAC-T cells with scFvs could significantly attenuate the effect of apoptosis and necrosis compared with the negative control group. In vivo, the qPCR and ELISA results demonstrated that scFvs reduced the transcription and expression of Tumor Necrosis Factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-8, and IL-18. Histopathology and myeloperoxidase (MPO) results showed that scFvs ameliorated the histopathological damages and reduced the inflammatory cells infiltration. The overall results demonstrated the positive anti-inflammatory effect of scFvs, revealing the potential role of scFvs in the prevention and treatment of <i>S. aureus</i> infections.Lei ZhangXin YeYan ZhangFengqing WangFanqing ZhangYan JiaDangjin WuKalbinur TohtiManling ChengJianguo ZhuMDPI AGarticlebovine mastitis<i>Staphylococcus aureus</i> (<i>S. aureus</i>)single-chain fragment variable (scFv)bovine mammary epithelial (MAC-T) cellscytotoxicityanti-inflammatoryMedicineRENVaccines, Vol 9, Iss 1300, p 1300 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bovine mastitis <i>Staphylococcus aureus</i> (<i>S. aureus</i>) single-chain fragment variable (scFv) bovine mammary epithelial (MAC-T) cells cytotoxicity anti-inflammatory Medicine R
spellingShingle	bovine mastitis <i>Staphylococcus aureus</i> (<i>S. aureus</i>) single-chain fragment variable (scFv) bovine mammary epithelial (MAC-T) cells cytotoxicity anti-inflammatory Medicine R Lei Zhang Xin Ye Yan Zhang Fengqing Wang Fanqing Zhang Yan Jia Dangjin Wu Kalbinur Tohti Manling Cheng Jianguo Zhu Anti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo
description	<i>Staphylococcus aureus</i> is a causative agent of bovine mastitis, capable of causing significant economic losses to the dairy industry worldwide. This study focuses on obtaining single-chain fragment variables (scFvs) against the virulence factors of <i>S. aureus</i> and evaluates the protective effect of scFvs on bovine mammary epithelial (MAC-T) cells and mice mammary gland tissues infected by <i>S. aureus</i>. After five rounds of bio-panning, four scFvs targeting four virulence factors of <i>S. aureus</i> were obtained. The complementarity-determining regions (CDRs) of these scFvs exhibited significant diversities, especially CDR3 of the VH domain. In vitro, each of scFvs was capable of inhibiting <i>S. aureus</i> growth and reducing the damage of MAC-T cells infected by <i>S. aureus</i>. Preincubation of MAC-T cells with scFvs could significantly attenuate the effect of apoptosis and necrosis compared with the negative control group. In vivo, the qPCR and ELISA results demonstrated that scFvs reduced the transcription and expression of Tumor Necrosis Factor alpha (TNF-α), interleukin-1β (IL-1β), IL-6, IL-8, and IL-18. Histopathology and myeloperoxidase (MPO) results showed that scFvs ameliorated the histopathological damages and reduced the inflammatory cells infiltration. The overall results demonstrated the positive anti-inflammatory effect of scFvs, revealing the potential role of scFvs in the prevention and treatment of <i>S. aureus</i> infections.
format	article
author	Lei Zhang Xin Ye Yan Zhang Fengqing Wang Fanqing Zhang Yan Jia Dangjin Wu Kalbinur Tohti Manling Cheng Jianguo Zhu
author_facet	Lei Zhang Xin Ye Yan Zhang Fengqing Wang Fanqing Zhang Yan Jia Dangjin Wu Kalbinur Tohti Manling Cheng Jianguo Zhu
author_sort	Lei Zhang
title	Anti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo
title_short	Anti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo
title_full	Anti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo
title_fullStr	Anti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo
title_full_unstemmed	Anti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo
title_sort	anti-<i>staphylococcus aureus</i> single-chain fragment variables play a protective anti-inflammatory role in vitro and in vivo
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6a6da6ed37cc49b687b4bd52087df297
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Anti-<i>Staphylococcus aureus</i> Single-Chain Fragment Variables Play a Protective Anti-Inflammatory Role In Vitro and In Vivo

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