Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.

Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm(2))-induced apoptosis were inves...

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Autores principales:	Yoko Yoshihisa, Mariame Ali Hassan, Yukihiro Furusawa, Yoshiaki Tabuchi, Takashi Kondo, Tadamichi Shimizu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a6ed1781b33464183861b70e6b6ce1c
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spelling	oai:doaj.org-article:6a6ed1781b33464183861b70e6b6ce1c2021-11-18T08:11:24ZAlkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.1932-620310.1371/journal.pone.0047903https://doaj.org/article/6a6ed1781b33464183861b70e6b6ce1c2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23110127/?tool=EBIhttps://doaj.org/toc/1932-6203Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm(2))-induced apoptosis were investigated in human keratinocyte HaCaT cells. Pretreatment of cells with alkannin (1 µM) caused significant inhibition of UVB-induced apoptosis and caspase-3 cleavage. On the other hand, the addition of KNK437 (HSP70 inhibitor) reversed the action of alkannin increasing UVB-induced apoptosis in a dose-dependent manner. In addition, differences in gene expression associated with the suppression of UVB-induced apoptosis in the presence of alkannin were investigated using Gene Chip assay. Our results indicate that alkannin suppresses UVB-induced apoptosis through the induction of HSP70 in human keratinocytes, and therefore, we suggest the usefulness of using alkannin as an antiaging agent.Yoko YoshihisaMariame Ali HassanYukihiro FurusawaYoshiaki TabuchiTakashi KondoTadamichi ShimizuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47903 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yoko Yoshihisa Mariame Ali Hassan Yukihiro Furusawa Yoshiaki Tabuchi Takashi Kondo Tadamichi Shimizu Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.
description	Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as a heat shock protein 70 (HSP70) inducer in living organisms. Here, the effects of alkannin-induced HSP70 on ultraviolet (UV) B (40 mJ/cm(2))-induced apoptosis were investigated in human keratinocyte HaCaT cells. Pretreatment of cells with alkannin (1 µM) caused significant inhibition of UVB-induced apoptosis and caspase-3 cleavage. On the other hand, the addition of KNK437 (HSP70 inhibitor) reversed the action of alkannin increasing UVB-induced apoptosis in a dose-dependent manner. In addition, differences in gene expression associated with the suppression of UVB-induced apoptosis in the presence of alkannin were investigated using Gene Chip assay. Our results indicate that alkannin suppresses UVB-induced apoptosis through the induction of HSP70 in human keratinocytes, and therefore, we suggest the usefulness of using alkannin as an antiaging agent.
format	article
author	Yoko Yoshihisa Mariame Ali Hassan Yukihiro Furusawa Yoshiaki Tabuchi Takashi Kondo Tadamichi Shimizu
author_facet	Yoko Yoshihisa Mariame Ali Hassan Yukihiro Furusawa Yoshiaki Tabuchi Takashi Kondo Tadamichi Shimizu
author_sort	Yoko Yoshihisa
title	Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.
title_short	Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.
title_full	Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.
title_fullStr	Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.
title_full_unstemmed	Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.
title_sort	alkannin, hsp70 inducer, protects against uvb-induced apoptosis in human keratinocytes.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6a6ed1781b33464183861b70e6b6ce1c
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Alkannin, HSP70 inducer, protects against UVB-induced apoptosis in human keratinocytes.

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