Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm

Abstract Cytoplasmic male sterility(CMS), a maternally inherited trait, provides a promising means to harness yield gains associated with hybrid vigor. In pigeonpea [Cajanus cajan (L.) Huth], nine types of sterility‐inducing cytoplasm have been reported, of which A2 and A4 have been successfully dep...

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Autores principales:	Abhishek Bohra, Gandam Prasad, Abhishek Rathore, Rachit K Saxena, Satheesh Naik SJ, Shalini Pareek, Rintu Jha, Lekha Pazhamala, Dibendu Datta, Gaurav Pandey, Abha Tiwari, Alok Kumar Maurya, Khela Ram Soren, Mohd Akram, Rajeev K Varshney, Narendra P Singh
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Plant culture SB1-1110 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/6a7459fa4c2f41be864215f671914c01
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spelling	oai:doaj.org-article:6a7459fa4c2f41be864215f671914c012021-12-05T07:50:12ZGlobal gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm1940-337210.1002/tpg2.20132https://doaj.org/article/6a7459fa4c2f41be864215f671914c012021-11-01T00:00:00Zhttps://doi.org/10.1002/tpg2.20132https://doaj.org/toc/1940-3372Abstract Cytoplasmic male sterility(CMS), a maternally inherited trait, provides a promising means to harness yield gains associated with hybrid vigor. In pigeonpea [Cajanus cajan (L.) Huth], nine types of sterility‐inducing cytoplasm have been reported, of which A2 and A4 have been successfully deployed in hybrid breeding. Unfortunately, molecular mechanism of the CMS trait is poorly understood because of limited research invested. More recently, an association between a mitochondrial gene (nad7) and A4‐CMS has been demonstrated in pigeonpea; however, the mechanism underlying A2‐CMS still remains obscure. The current investigation aimed to analyze the differences in A2‐CMS line (ICPL 88039A) and its isogenic maintainer line (ICPL 88039B) at transcriptome level using next‐generation sequencing. Gene expression profiling uncovered a set of 505 genes that showed altered expression in response to CMS, of which, 412 genes were upregulated while 93 were downregulated in the fertile maintainer line vs. the CMS line. Further, gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) network analyses revealed association of CMS in pigeonpea with four major pathways: glucose and lipid metabolism, ATP production, pollen development and pollen tube growth, and reactive oxygen species (ROS) scavenging. Patterns of digital gene expression were confirmed by quantitative real‐time polymerase chain reaction (qRT‐PCR) of six candidate genes. This study elucidates candidate genes and metabolic pathways having potential associations with pollen development and male sterility in pigeonpea A2‐CMS. New insights on molecular mechanism of CMS trait in pigeonpea will be helpful to accelerate heterosis utilization for enhancing productivity gains in pigeonpea.Abhishek BohraGandam PrasadAbhishek RathoreRachit K SaxenaSatheesh Naik SJShalini PareekRintu JhaLekha PazhamalaDibendu DattaGaurav PandeyAbha TiwariAlok Kumar MauryaKhela Ram SorenMohd AkramRajeev K VarshneyNarendra P SinghWileyarticlePlant cultureSB1-1110GeneticsQH426-470ENThe Plant Genome, Vol 14, Iss 3, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plant culture SB1-1110 Genetics QH426-470
spellingShingle	Plant culture SB1-1110 Genetics QH426-470 Abhishek Bohra Gandam Prasad Abhishek Rathore Rachit K Saxena Satheesh Naik SJ Shalini Pareek Rintu Jha Lekha Pazhamala Dibendu Datta Gaurav Pandey Abha Tiwari Alok Kumar Maurya Khela Ram Soren Mohd Akram Rajeev K Varshney Narendra P Singh Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm
description	Abstract Cytoplasmic male sterility(CMS), a maternally inherited trait, provides a promising means to harness yield gains associated with hybrid vigor. In pigeonpea [Cajanus cajan (L.) Huth], nine types of sterility‐inducing cytoplasm have been reported, of which A2 and A4 have been successfully deployed in hybrid breeding. Unfortunately, molecular mechanism of the CMS trait is poorly understood because of limited research invested. More recently, an association between a mitochondrial gene (nad7) and A4‐CMS has been demonstrated in pigeonpea; however, the mechanism underlying A2‐CMS still remains obscure. The current investigation aimed to analyze the differences in A2‐CMS line (ICPL 88039A) and its isogenic maintainer line (ICPL 88039B) at transcriptome level using next‐generation sequencing. Gene expression profiling uncovered a set of 505 genes that showed altered expression in response to CMS, of which, 412 genes were upregulated while 93 were downregulated in the fertile maintainer line vs. the CMS line. Further, gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) network analyses revealed association of CMS in pigeonpea with four major pathways: glucose and lipid metabolism, ATP production, pollen development and pollen tube growth, and reactive oxygen species (ROS) scavenging. Patterns of digital gene expression were confirmed by quantitative real‐time polymerase chain reaction (qRT‐PCR) of six candidate genes. This study elucidates candidate genes and metabolic pathways having potential associations with pollen development and male sterility in pigeonpea A2‐CMS. New insights on molecular mechanism of CMS trait in pigeonpea will be helpful to accelerate heterosis utilization for enhancing productivity gains in pigeonpea.
format	article
author	Abhishek Bohra Gandam Prasad Abhishek Rathore Rachit K Saxena Satheesh Naik SJ Shalini Pareek Rintu Jha Lekha Pazhamala Dibendu Datta Gaurav Pandey Abha Tiwari Alok Kumar Maurya Khela Ram Soren Mohd Akram Rajeev K Varshney Narendra P Singh
author_facet	Abhishek Bohra Gandam Prasad Abhishek Rathore Rachit K Saxena Satheesh Naik SJ Shalini Pareek Rintu Jha Lekha Pazhamala Dibendu Datta Gaurav Pandey Abha Tiwari Alok Kumar Maurya Khela Ram Soren Mohd Akram Rajeev K Varshney Narendra P Singh
author_sort	Abhishek Bohra
title	Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm
title_short	Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm
title_full	Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm
title_fullStr	Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm
title_full_unstemmed	Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm
title_sort	global gene expression analysis of pigeonpea with male sterility conditioned by a2 cytoplasm
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/6a7459fa4c2f41be864215f671914c01
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Global gene expression analysis of pigeonpea with male sterility conditioned by A2 cytoplasm

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