Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia

Katili AS, Retnowati Y. 2017. Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas 18: 826-833. Actinomycetes is a group of positive gram microorganism known to produce secondary metabolic compounds that work as antibiotics, anti-f...

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Autores principales: ABUBAKAR SIDIK KATILI, YULIANA RETNOWATI
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Lenguaje:EN
Publicado: MBI & UNS Solo 2017
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spelling oai:doaj.org-article:6a79c65d24cc4ac2bd0784c92bfe28c52021-11-16T13:52:30ZShort Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia1412-033X2085-472210.13057/biodiv/d180259https://doaj.org/article/6a79c65d24cc4ac2bd0784c92bfe28c52017-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/1718https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Katili AS, Retnowati Y. 2017. Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas 18: 826-833. Actinomycetes is a group of positive gram microorganism known to produce secondary metabolic compounds that work as antibiotics, anti-fungus, anti-virus, anti-cancer, and other important enzymes for the industry. This research aimed at discovering the potentials of Actinomycetes of the mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. It focused on finding out the diversity of Actinomycetes at mangrove ecosystem in Torosiaje. Hence, various types of Actinomycetes that have potentials to create secondary metabolic compounds/bioactive compounds for industrial purposes can be found. This purpose can only be attained through isolation of actinomycetes. This isolation was conducted through characterization of morphological characteristics of the colony and the spore, molecular characterization through isolation of DNA genome of the actinomycetes, amplification of 16S rRNA through PCR, sequencing, and reconstruction of the phylogenetic tree. We were able to obtain the actinomycetes isolate from the sediment in rhizosphere area of Ceriops tagal, Bruguiera gymnorrhiza, Xylocarpus sp, Rhizophora apiculata, Avicennia sp, and Sonneratia alba species of mangrove. Each isolated actinomycetes has specific morphological characteristics. The density of isolated actinomycetes in Torosiaje mangrove area was very low with an average of 1 x 105 CFU gram-1 in each sediment.ABUBAKAR SIDIK KATILIYULIANA RETNOWATIMBI & UNS SoloarticleactinomycetesdiversitymangroveBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 2, Pp 826-833 (2017)
institution DOAJ
collection DOAJ
language EN
topic actinomycetes
diversity
mangrove
Biology (General)
QH301-705.5
spellingShingle actinomycetes
diversity
mangrove
Biology (General)
QH301-705.5
ABUBAKAR SIDIK KATILI
YULIANA RETNOWATI
Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia
description Katili AS, Retnowati Y. 2017. Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. Biodiversitas 18: 826-833. Actinomycetes is a group of positive gram microorganism known to produce secondary metabolic compounds that work as antibiotics, anti-fungus, anti-virus, anti-cancer, and other important enzymes for the industry. This research aimed at discovering the potentials of Actinomycetes of the mangrove ecosystem in Torosiaje, Gorontalo, Indonesia. It focused on finding out the diversity of Actinomycetes at mangrove ecosystem in Torosiaje. Hence, various types of Actinomycetes that have potentials to create secondary metabolic compounds/bioactive compounds for industrial purposes can be found. This purpose can only be attained through isolation of actinomycetes. This isolation was conducted through characterization of morphological characteristics of the colony and the spore, molecular characterization through isolation of DNA genome of the actinomycetes, amplification of 16S rRNA through PCR, sequencing, and reconstruction of the phylogenetic tree. We were able to obtain the actinomycetes isolate from the sediment in rhizosphere area of Ceriops tagal, Bruguiera gymnorrhiza, Xylocarpus sp, Rhizophora apiculata, Avicennia sp, and Sonneratia alba species of mangrove. Each isolated actinomycetes has specific morphological characteristics. The density of isolated actinomycetes in Torosiaje mangrove area was very low with an average of 1 x 105 CFU gram-1 in each sediment.
format article
author ABUBAKAR SIDIK KATILI
YULIANA RETNOWATI
author_facet ABUBAKAR SIDIK KATILI
YULIANA RETNOWATI
author_sort ABUBAKAR SIDIK KATILI
title Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia
title_short Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia
title_full Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia
title_fullStr Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia
title_full_unstemmed Short Communication: Isolation of Actinomycetes from mangrove ecosystem in Torosiaje, Gorontalo, Indonesia
title_sort short communication: isolation of actinomycetes from mangrove ecosystem in torosiaje, gorontalo, indonesia
publisher MBI & UNS Solo
publishDate 2017
url https://doaj.org/article/6a79c65d24cc4ac2bd0784c92bfe28c5
work_keys_str_mv AT abubakarsidikkatili shortcommunicationisolationofactinomycetesfrommangroveecosystemintorosiajegorontaloindonesia
AT yulianaretnowati shortcommunicationisolationofactinomycetesfrommangroveecosystemintorosiajegorontaloindonesia
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