In situ modelling of biofilm formation in a hydrothermal spring cave

Abstract Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine bi...

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Autores principales:	Dóra Anda, Attila Szabó, Petra Kovács-Bodor, Judit Makk, Tamás Felföldi, Éva Ács, Judit Mádl-Szőnyi, Andrea K. Borsodi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a8a73e80c68428ea1f9bd82e05e0a56
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spelling	oai:doaj.org-article:6a8a73e80c68428ea1f9bd82e05e0a562021-12-02T15:11:51ZIn situ modelling of biofilm formation in a hydrothermal spring cave10.1038/s41598-020-78759-42045-2322https://doaj.org/article/6a8a73e80c68428ea1f9bd82e05e0a562020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78759-4https://doaj.org/toc/2045-2322Abstract Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine biofilm development for a year under controlled conditions in quasi-stagnant water of a hydrothermal spring cave located in the Buda Thermal Karst System (Hungary). Using a model system, we investigated how the structure of the biofilm is formed from the water and also how the growth rate of biofilm development takes place in this environment. Besides scanning electron microscopy, next-generation DNA sequencing was used to reveal the characteristic taxa and major shifts in the composition of the bacterial communities. Dynamic temporal changes were observed in the structure of bacterial communities. Bacterial richness and diversity increased during the biofilm formation, and 9–12 weeks were needed for the maturation. Increasing EPS production was also observed from the 9–12 weeks. The biofilm was different from the water that filled the cave pool, in terms of the taxonomic composition and metabolic potential of microorganisms. In these karstic environments, the formation of mature biofilm appears to take place relatively quickly, in a few months.Dóra AndaAttila SzabóPetra Kovács-BodorJudit MakkTamás FelföldiÉva ÁcsJudit Mádl-SzőnyiAndrea K. BorsodiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dóra Anda Attila Szabó Petra Kovács-Bodor Judit Makk Tamás Felföldi Éva Ács Judit Mádl-Szőnyi Andrea K. Borsodi In situ modelling of biofilm formation in a hydrothermal spring cave
description	Abstract Attachment of microorganisms to natural or artificial surfaces and the development of biofilms are complex processes which can be influenced by several factors. Nevertheless, our knowledge on biofilm formation in karstic environment is quite incomplete. The present study aimed to examine biofilm development for a year under controlled conditions in quasi-stagnant water of a hydrothermal spring cave located in the Buda Thermal Karst System (Hungary). Using a model system, we investigated how the structure of the biofilm is formed from the water and also how the growth rate of biofilm development takes place in this environment. Besides scanning electron microscopy, next-generation DNA sequencing was used to reveal the characteristic taxa and major shifts in the composition of the bacterial communities. Dynamic temporal changes were observed in the structure of bacterial communities. Bacterial richness and diversity increased during the biofilm formation, and 9–12 weeks were needed for the maturation. Increasing EPS production was also observed from the 9–12 weeks. The biofilm was different from the water that filled the cave pool, in terms of the taxonomic composition and metabolic potential of microorganisms. In these karstic environments, the formation of mature biofilm appears to take place relatively quickly, in a few months.
format	article
author	Dóra Anda Attila Szabó Petra Kovács-Bodor Judit Makk Tamás Felföldi Éva Ács Judit Mádl-Szőnyi Andrea K. Borsodi
author_facet	Dóra Anda Attila Szabó Petra Kovács-Bodor Judit Makk Tamás Felföldi Éva Ács Judit Mádl-Szőnyi Andrea K. Borsodi
author_sort	Dóra Anda
title	In situ modelling of biofilm formation in a hydrothermal spring cave
title_short	In situ modelling of biofilm formation in a hydrothermal spring cave
title_full	In situ modelling of biofilm formation in a hydrothermal spring cave
title_fullStr	In situ modelling of biofilm formation in a hydrothermal spring cave
title_full_unstemmed	In situ modelling of biofilm formation in a hydrothermal spring cave
title_sort	in situ modelling of biofilm formation in a hydrothermal spring cave
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/6a8a73e80c68428ea1f9bd82e05e0a56
work_keys_str_mv	AT doraanda insitumodellingofbiofilmformationinahydrothermalspringcave AT attilaszabo insitumodellingofbiofilmformationinahydrothermalspringcave AT petrakovacsbodor insitumodellingofbiofilmformationinahydrothermalspringcave AT juditmakk insitumodellingofbiofilmformationinahydrothermalspringcave AT tamasfelfoldi insitumodellingofbiofilmformationinahydrothermalspringcave AT evaacs insitumodellingofbiofilmformationinahydrothermalspringcave AT juditmadlszonyi insitumodellingofbiofilmformationinahydrothermalspringcave AT andreakborsodi insitumodellingofbiofilmformationinahydrothermalspringcave
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In situ modelling of biofilm formation in a hydrothermal spring cave

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