Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145

Abstract The Streptomyces genus is well known for its ability to produce bio-active secondary metabolites of great medical interest. However, the metabolic features accompanying these bio-productions remain to be defined. In this study, the comparison of related model strains producing differing lev...

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Autores principales:	Catherine Esnault, Thierry Dulermo, Aleksey Smirnov, Ahmed Askora, Michelle David, Ariane Deniset-Besseau, Ian-Barry Holland, Marie-Joelle Virolle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6a91ec3c03294680b99a8acfae5ce4af
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spelling	oai:doaj.org-article:6a91ec3c03294680b99a8acfae5ce4af2021-12-02T12:30:52ZStrong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M14510.1038/s41598-017-00259-92045-2322https://doaj.org/article/6a91ec3c03294680b99a8acfae5ce4af2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00259-9https://doaj.org/toc/2045-2322Abstract The Streptomyces genus is well known for its ability to produce bio-active secondary metabolites of great medical interest. However, the metabolic features accompanying these bio-productions remain to be defined. In this study, the comparison of related model strains producing differing levels of actinorhoddin (ACT), showed that S. lividans, a weak producer, had high TriAcylGlycerol (TAG) content indicative of a glycolytic metabolism. In contrast, the strong producer, S. coelicolor, was characterized by low TAG content, active consumption of its polyphosphate (PolyP) stores and extremely high ATP/ADP ratios. This indicated highly active oxidative metabolism that was correlated with induction of ACT biosynthesis. Interestingly, in conditions of phosphate limitation, the ppk mutant had TAG content and ACT production levels intermediary between those of S. lividans and S. coelicolor. This strain was characterized by high ADP levels indicating that Ppk was acting as an Adenosine Di Phosphate Kinase. Its absence resulted in energetic stress that is proposed to trigger an activation of oxidative metabolism to restore its energetic balance. This process, which is correlated with ACT biosynthesis, requires acetylCoA to fuel the Krebs cycle and phosphate for ATP generation by the ATP synthase coupled to the respiratory chain, resulting in low TAG and polyP content of the ACT producing strains.Catherine EsnaultThierry DulermoAleksey SmirnovAhmed AskoraMichelle DavidAriane Deniset-BesseauIan-Barry HollandMarie-Joelle VirolleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Catherine Esnault Thierry Dulermo Aleksey Smirnov Ahmed Askora Michelle David Ariane Deniset-Besseau Ian-Barry Holland Marie-Joelle Virolle Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145
description	Abstract The Streptomyces genus is well known for its ability to produce bio-active secondary metabolites of great medical interest. However, the metabolic features accompanying these bio-productions remain to be defined. In this study, the comparison of related model strains producing differing levels of actinorhoddin (ACT), showed that S. lividans, a weak producer, had high TriAcylGlycerol (TAG) content indicative of a glycolytic metabolism. In contrast, the strong producer, S. coelicolor, was characterized by low TAG content, active consumption of its polyphosphate (PolyP) stores and extremely high ATP/ADP ratios. This indicated highly active oxidative metabolism that was correlated with induction of ACT biosynthesis. Interestingly, in conditions of phosphate limitation, the ppk mutant had TAG content and ACT production levels intermediary between those of S. lividans and S. coelicolor. This strain was characterized by high ADP levels indicating that Ppk was acting as an Adenosine Di Phosphate Kinase. Its absence resulted in energetic stress that is proposed to trigger an activation of oxidative metabolism to restore its energetic balance. This process, which is correlated with ACT biosynthesis, requires acetylCoA to fuel the Krebs cycle and phosphate for ATP generation by the ATP synthase coupled to the respiratory chain, resulting in low TAG and polyP content of the ACT producing strains.
format	article
author	Catherine Esnault Thierry Dulermo Aleksey Smirnov Ahmed Askora Michelle David Ariane Deniset-Besseau Ian-Barry Holland Marie-Joelle Virolle
author_facet	Catherine Esnault Thierry Dulermo Aleksey Smirnov Ahmed Askora Michelle David Ariane Deniset-Besseau Ian-Barry Holland Marie-Joelle Virolle
author_sort	Catherine Esnault
title	Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145
title_short	Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145
title_full	Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145
title_fullStr	Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145
title_full_unstemmed	Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145
title_sort	strong antibiotic production is correlated with highly active oxidative metabolism in streptomyces coelicolor m145
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6a91ec3c03294680b99a8acfae5ce4af
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Strong antibiotic production is correlated with highly active oxidative metabolism in Streptomyces coelicolor M145

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