Orthogonal translation enables heterologous ribosome engineering in E. coli

Synthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Natalie S. Kolber, Ranan Fattal, Sinisa Bratulic, Gavriela D. Carver, Ahmed H. Badran
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6aa4a18ef0ce446fb94992971a6c0623
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6aa4a18ef0ce446fb94992971a6c0623
record_format	dspace
spelling	oai:doaj.org-article:6aa4a18ef0ce446fb94992971a6c06232021-12-02T13:57:31ZOrthogonal translation enables heterologous ribosome engineering in E. coli10.1038/s41467-020-20759-z2041-1723https://doaj.org/article/6aa4a18ef0ce446fb94992971a6c06232021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20759-zhttps://doaj.org/toc/2041-1723Synthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize their function.Natalie S. KolberRanan FattalSinisa BratulicGavriela D. CarverAhmed H. BadranNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Natalie S. Kolber Ranan Fattal Sinisa Bratulic Gavriela D. Carver Ahmed H. Badran Orthogonal translation enables heterologous ribosome engineering in E. coli
description	Synthetic biologists often co-opt heterologous parts to affect new functions in living cells, yet such an approach has rarely been extended to structural components of the ribosome. Here, the authors describe generalizable methods to express ribosomes from divergent microbes in E. coli and maximize their function.
format	article
author	Natalie S. Kolber Ranan Fattal Sinisa Bratulic Gavriela D. Carver Ahmed H. Badran
author_facet	Natalie S. Kolber Ranan Fattal Sinisa Bratulic Gavriela D. Carver Ahmed H. Badran
author_sort	Natalie S. Kolber
title	Orthogonal translation enables heterologous ribosome engineering in E. coli
title_short	Orthogonal translation enables heterologous ribosome engineering in E. coli
title_full	Orthogonal translation enables heterologous ribosome engineering in E. coli
title_fullStr	Orthogonal translation enables heterologous ribosome engineering in E. coli
title_full_unstemmed	Orthogonal translation enables heterologous ribosome engineering in E. coli
title_sort	orthogonal translation enables heterologous ribosome engineering in e. coli
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6aa4a18ef0ce446fb94992971a6c0623
work_keys_str_mv	AT natalieskolber orthogonaltranslationenablesheterologousribosomeengineeringinecoli AT rananfattal orthogonaltranslationenablesheterologousribosomeengineeringinecoli AT sinisabratulic orthogonaltranslationenablesheterologousribosomeengineeringinecoli AT gavrieladcarver orthogonaltranslationenablesheterologousribosomeengineeringinecoli AT ahmedhbadran orthogonaltranslationenablesheterologousribosomeengineeringinecoli
_version_	1718392326654525440

Orthogonal translation enables heterologous ribosome engineering in E. coli

Ejemplares similares