Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats

Background: Osteoarthritis is the most common degenerative joint disease worldwide but its treatment can cause serious adverse events. Ethanol extract of peanut shells contains luteolin functioned as its main anti-inflammatory and chondroprotective agent. Apis dorsata honey also contains anti-inflam...

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Autores principales:	Bella Stevanny, Tungki Pratama Umar, Junoretta Haviva Ernanto, Rachmat Hidayat
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2020
Materias:	apis dorsata honey inflammation mmp-3 osteoarthritis peanut shells extract Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/6aac35ac89c64a93ad8b18c47b54a363
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spelling	oai:doaj.org-article:6aac35ac89c64a93ad8b18c47b54a3632021-11-05T16:47:43ZPeanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats2503-217810.14710/jbtr.v6i3.7908https://doaj.org/article/6aac35ac89c64a93ad8b18c47b54a3632020-12-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/7908https://doaj.org/toc/2503-2178Background: Osteoarthritis is the most common degenerative joint disease worldwide but its treatment can cause serious adverse events. Ethanol extract of peanut shells contains luteolin functioned as its main anti-inflammatory and chondroprotective agent. Apis dorsata honey also contains anti-inflammatory and antioxidant agent, especially from Tualang honey type. Both have the potential to reduce inflammation and prevent articular degradation in osteoarthritis. Objective: This study aims to determine the effect of peanut shells extract and Apis dorsata honey on matrix metalloproteinase-3 (MMP-3) level in monosodium iodoacetate-induced (MIA) osteoarthritic rats. Methods: In this in vivo study, female Wistar rats (n=27) were randomly divided to nine groups containing three rats each. Treatment was given to group 1, 2, and 3: Tualang honey (TH) 25% + peanut shells extract (PSE) with 1%, 5%, and 10% concentration; group 4, 5, and 6: TH 50% + PSE with 1%, 5%, and 10% concentration; group 7: diclofenac sodium (positive control); group 8: aquadest (negative control); and group 9: aquadest (normal) for 10 days. We induced knee osteoarthritis by intraarticular injection of MIA in day 4. Anti-inflammatory and chondroprotective activities were evaluated with MMP-3 ELISA. Results: The mixture of peanut shells extract and Apis dorsata honey significantly reduced MMP-3 level in group 1 (331.12 pg/ml), group 2 (291.73 pg/ml), group 3 (266.58 pg/ml), group 4 (274.15 pg/ml), group 5 (251.12 pg/ml), and group 6 (220.52 pg/ml) after 10 days of treatment. MMP-3 level was also evaluated in group 7 (169.61 pg/ml), group 8 (413.55 pg/ml), and group 9 (39 pg/ml). Compared to the negative control group, treatment and diclofenac groups showed significant effect in reducing MMP-3 level in patello-femoral articular cartilage. Conclusion: Peanut shells extract and Apis dorsata honey showed antiinflammatory and chondroprotective effect by reducing MMP-3 level in MIA-induced osteoarthritic rats.Bella StevannyTungki Pratama UmarJunoretta Haviva ErnantoRachmat HidayatDiponegoro Universityarticleapis dorsata honeyinflammationmmp-3osteoarthritispeanut shells extractMedicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 6, Iss 3, Pp 74-78 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	apis dorsata honey inflammation mmp-3 osteoarthritis peanut shells extract Medicine (General) R5-920
spellingShingle	apis dorsata honey inflammation mmp-3 osteoarthritis peanut shells extract Medicine (General) R5-920 Bella Stevanny Tungki Pratama Umar Junoretta Haviva Ernanto Rachmat Hidayat Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats
description	Background: Osteoarthritis is the most common degenerative joint disease worldwide but its treatment can cause serious adverse events. Ethanol extract of peanut shells contains luteolin functioned as its main anti-inflammatory and chondroprotective agent. Apis dorsata honey also contains anti-inflammatory and antioxidant agent, especially from Tualang honey type. Both have the potential to reduce inflammation and prevent articular degradation in osteoarthritis. Objective: This study aims to determine the effect of peanut shells extract and Apis dorsata honey on matrix metalloproteinase-3 (MMP-3) level in monosodium iodoacetate-induced (MIA) osteoarthritic rats. Methods: In this in vivo study, female Wistar rats (n=27) were randomly divided to nine groups containing three rats each. Treatment was given to group 1, 2, and 3: Tualang honey (TH) 25% + peanut shells extract (PSE) with 1%, 5%, and 10% concentration; group 4, 5, and 6: TH 50% + PSE with 1%, 5%, and 10% concentration; group 7: diclofenac sodium (positive control); group 8: aquadest (negative control); and group 9: aquadest (normal) for 10 days. We induced knee osteoarthritis by intraarticular injection of MIA in day 4. Anti-inflammatory and chondroprotective activities were evaluated with MMP-3 ELISA. Results: The mixture of peanut shells extract and Apis dorsata honey significantly reduced MMP-3 level in group 1 (331.12 pg/ml), group 2 (291.73 pg/ml), group 3 (266.58 pg/ml), group 4 (274.15 pg/ml), group 5 (251.12 pg/ml), and group 6 (220.52 pg/ml) after 10 days of treatment. MMP-3 level was also evaluated in group 7 (169.61 pg/ml), group 8 (413.55 pg/ml), and group 9 (39 pg/ml). Compared to the negative control group, treatment and diclofenac groups showed significant effect in reducing MMP-3 level in patello-femoral articular cartilage. Conclusion: Peanut shells extract and Apis dorsata honey showed antiinflammatory and chondroprotective effect by reducing MMP-3 level in MIA-induced osteoarthritic rats.
format	article
author	Bella Stevanny Tungki Pratama Umar Junoretta Haviva Ernanto Rachmat Hidayat
author_facet	Bella Stevanny Tungki Pratama Umar Junoretta Haviva Ernanto Rachmat Hidayat
author_sort	Bella Stevanny
title	Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats
title_short	Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats
title_full	Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats
title_fullStr	Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats
title_full_unstemmed	Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats
title_sort	peanut (arachis hypogaea) shells extract and apis dorsata honey reduce matrix metalloproteinase-3 in monosodium iodoacetate-induced osteoarthritic rats
publisher	Diponegoro University
publishDate	2020
url	https://doaj.org/article/6aac35ac89c64a93ad8b18c47b54a363
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Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic Rats

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