Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress

The present study demonstrates the Ni toxicity-ameliorating and growth-promoting abilities of two different bacterial isolates when applied to wheat (Triticum aestivum L.) as the host plant. Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected ba...

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Autores principales:	Veronika N. Pishchik, Polina S. Filippova, Galina V. Mirskaya, Yuriy V. Khomyakov, Vitaliy E. Vertebny, Viktoriya I. Dubovitskaya, Yuliya V. Ostankova, Aleksandr V. Semenov, Debasis Chakrabarty, Evgeny V. Zuev, Vladimir K. Chebotar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	epiphytic PGPB <i>Bacillus megaterium</i> AFI1 <i>Paenibacillus nicotianae</i> AFI2 wheat (<i>Triticum aestivum</i> L.) Ni stress photosynthetic pigments Botany QK1-989
Acceso en línea:	https://doaj.org/article/6aae3a34c41d4c86849f84294ff2a6e1
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spelling	oai:doaj.org-article:6aae3a34c41d4c86849f84294ff2a6e12021-11-25T18:45:28ZEpiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress10.3390/plants101123342223-7747https://doaj.org/article/6aae3a34c41d4c86849f84294ff2a6e12021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2334https://doaj.org/toc/2223-7747The present study demonstrates the Ni toxicity-ameliorating and growth-promoting abilities of two different bacterial isolates when applied to wheat (<i>Triticum aestivum</i> L.) as the host plant. Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected based on their ability to improve the germination of wheat plants; they were identified as <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2. The protective effects of these epiphytic bacteria against Ni stress were studied in model experiments with two wheat cultivars: Ni stress-tolerant Leningradskaya 6 and susceptible Chinese spring. When these isolates were used as the inoculants applied to Ni-treated wheat plants, the growth parameters and the levels of photosynthetic pigments of the two wheat cultivars both under normal and Ni-stress conditions were increased, though <i>B. megaterium</i> AFI1 had a more pronounced ameliorative effect on the Ni contents in plant tissues due to its synthesis of siderophores. Over the 10 days of Ni exposure, the plant growth promotion bacteria (PGPB) significantly reduced the lipid peroxidation (LPO), ascorbate peroxidase (APX), superoxide dismutase (SOD) activities and proline content in the leaves of both wheat cultivars. The PGPB also increased peroxidase (POX) activity and the levels of chlorophyll <i>a</i>, chlorophyll <i>b</i>, and carotenoids in the wheat leaves. It was concluded that <i>B. megaterium</i> AFI1 is an ideal candidate for bioremediation and wheat growth promotion against Ni-induced oxidative stress, as it increases photosynthetic pigment contents, induces the antioxidant defense system, and lowers Ni metal uptake.Veronika N. PishchikPolina S. FilippovaGalina V. MirskayaYuriy V. KhomyakovVitaliy E. VertebnyViktoriya I. DubovitskayaYuliya V. OstankovaAleksandr V. SemenovDebasis ChakrabartyEvgeny V. ZuevVladimir K. ChebotarMDPI AGarticleepiphytic PGPB<i>Bacillus megaterium</i> AFI1<i>Paenibacillus nicotianae</i> AFI2wheat (<i>Triticum aestivum</i> L.)Ni stressphotosynthetic pigmentsBotanyQK1-989ENPlants, Vol 10, Iss 2334, p 2334 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	epiphytic PGPB <i>Bacillus megaterium</i> AFI1 <i>Paenibacillus nicotianae</i> AFI2 wheat (<i>Triticum aestivum</i> L.) Ni stress photosynthetic pigments Botany QK1-989
spellingShingle	epiphytic PGPB <i>Bacillus megaterium</i> AFI1 <i>Paenibacillus nicotianae</i> AFI2 wheat (<i>Triticum aestivum</i> L.) Ni stress photosynthetic pigments Botany QK1-989 Veronika N. Pishchik Polina S. Filippova Galina V. Mirskaya Yuriy V. Khomyakov Vitaliy E. Vertebny Viktoriya I. Dubovitskaya Yuliya V. Ostankova Aleksandr V. Semenov Debasis Chakrabarty Evgeny V. Zuev Vladimir K. Chebotar Epiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
description	The present study demonstrates the Ni toxicity-ameliorating and growth-promoting abilities of two different bacterial isolates when applied to wheat (<i>Triticum aestivum</i> L.) as the host plant. Two bacterial strains tolerant to Ni stress were isolated from wheat seeds and selected based on their ability to improve the germination of wheat plants; they were identified as <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2. The protective effects of these epiphytic bacteria against Ni stress were studied in model experiments with two wheat cultivars: Ni stress-tolerant Leningradskaya 6 and susceptible Chinese spring. When these isolates were used as the inoculants applied to Ni-treated wheat plants, the growth parameters and the levels of photosynthetic pigments of the two wheat cultivars both under normal and Ni-stress conditions were increased, though <i>B. megaterium</i> AFI1 had a more pronounced ameliorative effect on the Ni contents in plant tissues due to its synthesis of siderophores. Over the 10 days of Ni exposure, the plant growth promotion bacteria (PGPB) significantly reduced the lipid peroxidation (LPO), ascorbate peroxidase (APX), superoxide dismutase (SOD) activities and proline content in the leaves of both wheat cultivars. The PGPB also increased peroxidase (POX) activity and the levels of chlorophyll <i>a</i>, chlorophyll <i>b</i>, and carotenoids in the wheat leaves. It was concluded that <i>B. megaterium</i> AFI1 is an ideal candidate for bioremediation and wheat growth promotion against Ni-induced oxidative stress, as it increases photosynthetic pigment contents, induces the antioxidant defense system, and lowers Ni metal uptake.
format	article
author	Veronika N. Pishchik Polina S. Filippova Galina V. Mirskaya Yuriy V. Khomyakov Vitaliy E. Vertebny Viktoriya I. Dubovitskaya Yuliya V. Ostankova Aleksandr V. Semenov Debasis Chakrabarty Evgeny V. Zuev Vladimir K. Chebotar
author_facet	Veronika N. Pishchik Polina S. Filippova Galina V. Mirskaya Yuriy V. Khomyakov Vitaliy E. Vertebny Viktoriya I. Dubovitskaya Yuliya V. Ostankova Aleksandr V. Semenov Debasis Chakrabarty Evgeny V. Zuev Vladimir K. Chebotar
author_sort	Veronika N. Pishchik
title	Epiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_short	Epiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_full	Epiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_fullStr	Epiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_full_unstemmed	Epiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress
title_sort	epiphytic pgpb <i>bacillus megaterium</i> afi1 and <i>paenibacillus nicotianae</i> afi2 improve wheat growth and antioxidant status under ni stress
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/6aae3a34c41d4c86849f84294ff2a6e1
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Epiphytic PGPB <i>Bacillus megaterium</i> AFI1 and <i>Paenibacillus nicotianae</i> AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress

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