Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.

MicroRNAs are key modulators at molecular level in different biological processes, including determination of cell fate and differentiation. Herein, microRNA expression profiling experiments were performed on syngeneic cardiac (CStC) and bone marrow (BMStC) mesenchymal stromal cells cultured in stan...

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Autores principales:	Viviana Meraviglia, Valerio Azzimato, Luca Piacentini, Mattia Chiesa, Rupesh K Kesharwani, Caterina Frati, Maurizio C Capogrossi, Carlo Gaetano, Giulio Pompilio, Gualtiero I Colombo, Alessandra Rossini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ab3ebe0a49e4c2098c94b81751c22fe
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spelling	oai:doaj.org-article:6ab3ebe0a49e4c2098c94b81751c22fe2021-11-25T06:00:15ZSyngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.1932-620310.1371/journal.pone.0107269https://doaj.org/article/6ab3ebe0a49e4c2098c94b81751c22fe2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107269https://doaj.org/toc/1932-6203MicroRNAs are key modulators at molecular level in different biological processes, including determination of cell fate and differentiation. Herein, microRNA expression profiling experiments were performed on syngeneic cardiac (CStC) and bone marrow (BMStC) mesenchymal stromal cells cultured in standard growth medium and then in vitro exposed to adipogenic, osteogenic, cardiomyogenic and endothelial differentiation media. Analysis identified a tissue-specific microRNA signature composed of 16 microRNAs that univocally discriminated cell type of origin and that were completely unaffected by in vitro differentiation media: 4 microRNAs were over-expressed in cardiac stromal cells, and 12 were overexpressed or present only in bone marrow stromal cells. Further, results revealed microRNA subsets specifically modulated by each differentiation medium, irrespective of the cell type of origin, and a subset of 7 microRNAs that were down-regulated by all media with respect to growth medium. Finally, we identified 16 microRNAs that were differentially modulated by the media when comparing the two tissues of origin. The existence of a tissue-specific microRNA signature surviving to any differentiation stimuli, strongly support the role if microRNAs determining cell identity related to tissue origin. Moreover, we identified microRNA subsets modulated by different culture conditions in a tissue-specific manner, pointing out their importance during differentiation processes.Viviana MeravigliaValerio AzzimatoLuca PiacentiniMattia ChiesaRupesh K KesharwaniCaterina FratiMaurizio C CapogrossiCarlo GaetanoGiulio PompilioGualtiero I ColomboAlessandra RossiniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107269 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Viviana Meraviglia Valerio Azzimato Luca Piacentini Mattia Chiesa Rupesh K Kesharwani Caterina Frati Maurizio C Capogrossi Carlo Gaetano Giulio Pompilio Gualtiero I Colombo Alessandra Rossini Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.
description	MicroRNAs are key modulators at molecular level in different biological processes, including determination of cell fate and differentiation. Herein, microRNA expression profiling experiments were performed on syngeneic cardiac (CStC) and bone marrow (BMStC) mesenchymal stromal cells cultured in standard growth medium and then in vitro exposed to adipogenic, osteogenic, cardiomyogenic and endothelial differentiation media. Analysis identified a tissue-specific microRNA signature composed of 16 microRNAs that univocally discriminated cell type of origin and that were completely unaffected by in vitro differentiation media: 4 microRNAs were over-expressed in cardiac stromal cells, and 12 were overexpressed or present only in bone marrow stromal cells. Further, results revealed microRNA subsets specifically modulated by each differentiation medium, irrespective of the cell type of origin, and a subset of 7 microRNAs that were down-regulated by all media with respect to growth medium. Finally, we identified 16 microRNAs that were differentially modulated by the media when comparing the two tissues of origin. The existence of a tissue-specific microRNA signature surviving to any differentiation stimuli, strongly support the role if microRNAs determining cell identity related to tissue origin. Moreover, we identified microRNA subsets modulated by different culture conditions in a tissue-specific manner, pointing out their importance during differentiation processes.
format	article
author	Viviana Meraviglia Valerio Azzimato Luca Piacentini Mattia Chiesa Rupesh K Kesharwani Caterina Frati Maurizio C Capogrossi Carlo Gaetano Giulio Pompilio Gualtiero I Colombo Alessandra Rossini
author_facet	Viviana Meraviglia Valerio Azzimato Luca Piacentini Mattia Chiesa Rupesh K Kesharwani Caterina Frati Maurizio C Capogrossi Carlo Gaetano Giulio Pompilio Gualtiero I Colombo Alessandra Rossini
author_sort	Viviana Meraviglia
title	Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.
title_short	Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.
title_full	Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.
title_fullStr	Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.
title_full_unstemmed	Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.
title_sort	syngeneic cardiac and bone marrow stromal cells display tissue-specific microrna signatures and microrna subsets restricted to diverse differentiation processes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6ab3ebe0a49e4c2098c94b81751c22fe
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Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes.

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