High-resolution wide-field imaging of perfused capillaries without the use of contrast agent

Darin A Nelson1, Zvia Burgansky-Eliash1,2, Hila Barash1, Anat Loewenstein3, Adiel Barak4, Elisha Bartov2, Tali Rock2, Amiram Grinvald51Optical Imaging Ltd, Rehovot, Israel; 2Department of Ophthalmology, Edith Wolfson Medical Center, Holon, Israel; 3Department of Ophthalmology, Tel Aviv Medical Cente...

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Autores principales:	Nelson DA, Burgansky-Eliash Z, Barash H, Loewenstein A, Barak A, Bartov E, Rock T, Grinvald A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/6ab9b70df161436d88c8a5b52cec7243
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spelling	oai:doaj.org-article:6ab9b70df161436d88c8a5b52cec72432021-12-02T04:58:36ZHigh-resolution wide-field imaging of perfused capillaries without the use of contrast agent1177-54671177-5483https://doaj.org/article/6ab9b70df161436d88c8a5b52cec72432011-08-01T00:00:00Zhttp://www.dovepress.com/high-resolution-wide-field-imaging-of-perfused-capillaries-without-the-a8037https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Darin A Nelson1, Zvia Burgansky-Eliash1,2, Hila Barash1, Anat Loewenstein3, Adiel Barak4, Elisha Bartov2, Tali Rock2, Amiram Grinvald51Optical Imaging Ltd, Rehovot, Israel; 2Department of Ophthalmology, Edith Wolfson Medical Center, Holon, Israel; 3Department of Ophthalmology, Tel Aviv Medical Center &amp; Sackler Faculty of Medicine, Tel Aviv University, Israel; 4Department of Ophthalmology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel; 5Department of Neurobiology, The Weizmann Institute of Science, Rehovot, IsraelPurpose: Assessment of capillary abnormalities facilitates early diagnosis, treatment, and follow-up of common retinal pathologies. Injected contrast agents like fluorescein are widely used to image retinal capillaries, but this highly effective procedure has a few disadvantages, such as untoward side effects, inconvenience of injection, and brevity of the time window for clear visualization. The retinal function imager (RFI) is a tool for monitoring retinal functions, such as blood velocity and oximetry, based on intrinsic signals. Here we describe the clinical use of hemoglobin in red blood cells (RBCs) as an intrinsic motion-contrast agent in the generation of detailed noninvasive capillary-perfusion maps (nCPMs).Patients and methods: Multiple series of nCPM images were acquired from 130 patients with diabetic retinopathy, vein occlusion, central serous retinopathy, age-related macular degeneration, or metabolic syndrome, as well as from 37 healthy subjects. After registration, pixel value distribution parameters were analyzed to locate RBC motion.Results: The RFI yielded nCPMs demonstrating microvascular morphology including capillaries in exquisite detail. Maps from the same subject were highly reproducible in repeated measurements, in as much detail and often better than that revealed by the very best fluorescein angiography. In patients, neovascularization and capillary nonperfusion areas were clearly observed. Foveal avascular zones (FAZ) were sharply delineated and were larger in patients with diabetic retinopathy than in controls (FAZ diameter: 641.5 &plusmn; 82.3 versus 463.7 &plusmn; 105 &micro;m; P &lt; 0.001). Also visible were abnormal vascular patterns, such as shunts and vascular loops.Conclusion: Optical imaging of retinal capillaries in human patients based on motion contrast is noninvasive, comfortable, safe, and can be repeated as often as required for early diagnosis, treatment guidance, and follow up of retinal disease progression.Keywords: noninvasive, capillary maps, motion contrast, foveal avascular zone, retinal imaging, injection, fluoresceinNelson DABurgansky-Eliash ZBarash HLoewenstein ABarak ABartov ERock TGrinvald ADove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2011, Iss default, Pp 1095-1106 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Nelson DA Burgansky-Eliash Z Barash H Loewenstein A Barak A Bartov E Rock T Grinvald A High-resolution wide-field imaging of perfused capillaries without the use of contrast agent
description	Darin A Nelson1, Zvia Burgansky-Eliash1,2, Hila Barash1, Anat Loewenstein3, Adiel Barak4, Elisha Bartov2, Tali Rock2, Amiram Grinvald51Optical Imaging Ltd, Rehovot, Israel; 2Department of Ophthalmology, Edith Wolfson Medical Center, Holon, Israel; 3Department of Ophthalmology, Tel Aviv Medical Center &amp; Sackler Faculty of Medicine, Tel Aviv University, Israel; 4Department of Ophthalmology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel; 5Department of Neurobiology, The Weizmann Institute of Science, Rehovot, IsraelPurpose: Assessment of capillary abnormalities facilitates early diagnosis, treatment, and follow-up of common retinal pathologies. Injected contrast agents like fluorescein are widely used to image retinal capillaries, but this highly effective procedure has a few disadvantages, such as untoward side effects, inconvenience of injection, and brevity of the time window for clear visualization. The retinal function imager (RFI) is a tool for monitoring retinal functions, such as blood velocity and oximetry, based on intrinsic signals. Here we describe the clinical use of hemoglobin in red blood cells (RBCs) as an intrinsic motion-contrast agent in the generation of detailed noninvasive capillary-perfusion maps (nCPMs).Patients and methods: Multiple series of nCPM images were acquired from 130 patients with diabetic retinopathy, vein occlusion, central serous retinopathy, age-related macular degeneration, or metabolic syndrome, as well as from 37 healthy subjects. After registration, pixel value distribution parameters were analyzed to locate RBC motion.Results: The RFI yielded nCPMs demonstrating microvascular morphology including capillaries in exquisite detail. Maps from the same subject were highly reproducible in repeated measurements, in as much detail and often better than that revealed by the very best fluorescein angiography. In patients, neovascularization and capillary nonperfusion areas were clearly observed. Foveal avascular zones (FAZ) were sharply delineated and were larger in patients with diabetic retinopathy than in controls (FAZ diameter: 641.5 &plusmn; 82.3 versus 463.7 &plusmn; 105 &micro;m; P &lt; 0.001). Also visible were abnormal vascular patterns, such as shunts and vascular loops.Conclusion: Optical imaging of retinal capillaries in human patients based on motion contrast is noninvasive, comfortable, safe, and can be repeated as often as required for early diagnosis, treatment guidance, and follow up of retinal disease progression.Keywords: noninvasive, capillary maps, motion contrast, foveal avascular zone, retinal imaging, injection, fluorescein
format	article
author	Nelson DA Burgansky-Eliash Z Barash H Loewenstein A Barak A Bartov E Rock T Grinvald A
author_facet	Nelson DA Burgansky-Eliash Z Barash H Loewenstein A Barak A Bartov E Rock T Grinvald A
author_sort	Nelson DA
title	High-resolution wide-field imaging of perfused capillaries without the use of contrast agent
title_short	High-resolution wide-field imaging of perfused capillaries without the use of contrast agent
title_full	High-resolution wide-field imaging of perfused capillaries without the use of contrast agent
title_fullStr	High-resolution wide-field imaging of perfused capillaries without the use of contrast agent
title_full_unstemmed	High-resolution wide-field imaging of perfused capillaries without the use of contrast agent
title_sort	high-resolution wide-field imaging of perfused capillaries without the use of contrast agent
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/6ab9b70df161436d88c8a5b52cec7243
work_keys_str_mv	AT nelsonda highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent AT burganskyeliashz highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent AT barashh highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent AT loewensteina highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent AT baraka highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent AT bartove highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent AT rockt highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent AT grinvalda highresolutionwidefieldimagingofperfusedcapillarieswithouttheuseofcontrastagent
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High-resolution wide-field imaging of perfused capillaries without the use of contrast agent

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