Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.

Soluble Epoxide Hydrolase (sEH) is a key enzyme in the metabolism and termination of action of epoxyeicosatrienoic acids, derivatives of arachidonic acid, which are protective against ischemic stroke. Mice lacking sEH globally are protected from injury following stroke; however, little is known abou...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wenri Zhang, Catherine M Davis, Matthew L Edin, Craig R Lee, Darryl C Zeldin, Nabil J Alkayed
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ac0a2988f5f47159724fc3ac970fb84
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6ac0a2988f5f47159724fc3ac970fb84
record_format	dspace
spelling	oai:doaj.org-article:6ac0a2988f5f47159724fc3ac970fb842021-11-18T07:50:02ZRole of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.1932-620310.1371/journal.pone.0061244https://doaj.org/article/6ac0a2988f5f47159724fc3ac970fb842013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23585883/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Soluble Epoxide Hydrolase (sEH) is a key enzyme in the metabolism and termination of action of epoxyeicosatrienoic acids, derivatives of arachidonic acid, which are protective against ischemic stroke. Mice lacking sEH globally are protected from injury following stroke; however, little is known about the role of endothelial sEH in brain ischemia. We generated transgenic mice with endothelial-specific expression of human sEH (Tie2-hsEH), and assessed the effect of transgenic overexpression of endothelial sEH on endothelium-dependent vascular reactivity and ischemic injury following middle cerebral artery occlusion (MCAO). Compared to wild-type, male Tie2-hsEH mice exhibited impaired vasodilation in response to stimulation with 1 µM acetylcholine as assessed by laser-Doppler perfusion monitoring in an in-vivo cranial window preparation. No difference in infarct size was observed between wild-type and Tie2-hsEH male mice. In females, however, Tie2-hsEH mice sustained larger infarcts in striatum, but not cortex, compared to wild-type mice. Sex difference in ischemic injury was maintained in the cortex of Tie2-hsEH mice. In the striatum, expression of Tie2-hsEH resulted in a sex difference, with larger infarct in females than males. These findings demonstrate that transgenic expression of sEH in endothelium results in impaired endothelium-dependent vasodilation in the cerebral circulation, and that females are more susceptible to enhanced ischemic damage as a result of increased endothelial sEH than males, especially in end-arteriolar striatal region.Wenri ZhangCatherine M DavisMatthew L EdinCraig R LeeDarryl C ZeldinNabil J AlkayedPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61244 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenri Zhang Catherine M Davis Matthew L Edin Craig R Lee Darryl C Zeldin Nabil J Alkayed Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.
description	Soluble Epoxide Hydrolase (sEH) is a key enzyme in the metabolism and termination of action of epoxyeicosatrienoic acids, derivatives of arachidonic acid, which are protective against ischemic stroke. Mice lacking sEH globally are protected from injury following stroke; however, little is known about the role of endothelial sEH in brain ischemia. We generated transgenic mice with endothelial-specific expression of human sEH (Tie2-hsEH), and assessed the effect of transgenic overexpression of endothelial sEH on endothelium-dependent vascular reactivity and ischemic injury following middle cerebral artery occlusion (MCAO). Compared to wild-type, male Tie2-hsEH mice exhibited impaired vasodilation in response to stimulation with 1 µM acetylcholine as assessed by laser-Doppler perfusion monitoring in an in-vivo cranial window preparation. No difference in infarct size was observed between wild-type and Tie2-hsEH male mice. In females, however, Tie2-hsEH mice sustained larger infarcts in striatum, but not cortex, compared to wild-type mice. Sex difference in ischemic injury was maintained in the cortex of Tie2-hsEH mice. In the striatum, expression of Tie2-hsEH resulted in a sex difference, with larger infarct in females than males. These findings demonstrate that transgenic expression of sEH in endothelium results in impaired endothelium-dependent vasodilation in the cerebral circulation, and that females are more susceptible to enhanced ischemic damage as a result of increased endothelial sEH than males, especially in end-arteriolar striatal region.
format	article
author	Wenri Zhang Catherine M Davis Matthew L Edin Craig R Lee Darryl C Zeldin Nabil J Alkayed
author_facet	Wenri Zhang Catherine M Davis Matthew L Edin Craig R Lee Darryl C Zeldin Nabil J Alkayed
author_sort	Wenri Zhang
title	Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.
title_short	Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.
title_full	Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.
title_fullStr	Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.
title_full_unstemmed	Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.
title_sort	role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6ac0a2988f5f47159724fc3ac970fb84
work_keys_str_mv	AT wenrizhang roleofendothelialsolubleepoxidehydrolaseincerebrovascularfunctionandischemicinjury AT catherinemdavis roleofendothelialsolubleepoxidehydrolaseincerebrovascularfunctionandischemicinjury AT matthewledin roleofendothelialsolubleepoxidehydrolaseincerebrovascularfunctionandischemicinjury AT craigrlee roleofendothelialsolubleepoxidehydrolaseincerebrovascularfunctionandischemicinjury AT darrylczeldin roleofendothelialsolubleepoxidehydrolaseincerebrovascularfunctionandischemicinjury AT nabiljalkayed roleofendothelialsolubleepoxidehydrolaseincerebrovascularfunctionandischemicinjury
_version_	1718422916183359488

Role of endothelial soluble epoxide hydrolase in cerebrovascular function and ischemic injury.

Ejemplares similares