Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.

The unique properties of embryonic stem cells (ESC) rely on long-lasting self-renewal and their ability to switch in all adult cell type programs. Recent advances have shown that regulations at the chromatin level sustain both ESC properties along with transcription factors. We have focused our inte...

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Autores principales:	Maïa Caillier, Sandrine Thénot, Violaine Tribollet, Anne-Marie Birot, Jacques Samarut, Anne Mey
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ac0bc781c21472caac1536188915d29
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spelling	oai:doaj.org-article:6ac0bc781c21472caac1536188915d292021-11-18T07:36:52ZRole of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.1932-620310.1371/journal.pone.0015507https://doaj.org/article/6ac0bc781c21472caac1536188915d292010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21085495/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The unique properties of embryonic stem cells (ESC) rely on long-lasting self-renewal and their ability to switch in all adult cell type programs. Recent advances have shown that regulations at the chromatin level sustain both ESC properties along with transcription factors. We have focused our interest on the epigenetic modulator HP1γ (Heterochromatin Protein 1, isoform γ) that binds histones H3 methylated at lysine 9 (meH3K9) and is highly plastic in its distribution and association with the transcriptional regulation of specific genes during cell fate transitions. These characteristics of HP1γ make it a good candidate to sustain the ESC flexibility required for rapid program changes during differentiation. Using RNA interference, we describe the functional role of HP1γ in mouse ESC. The analysis of HP1γ deprived cells in proliferative and in various differentiating conditions was performed combining functional assays with molecular approaches (RT-qPCR, microarray). We show that HP1γ deprivation slows down the cell cycle of ESC and decreases their resistance to differentiating conditions, rendering the cells poised to differentiate. In addition, HP1γ depletion hampers the differentiation to the endoderm as compared with the differentiation to the neurectoderm or the mesoderm. Altogether, our results reveal the role of HP1γ in ESC self-renewal and in the balance between the pluripotent and the differentiation programs.Maïa CaillierSandrine ThénotViolaine TribolletAnne-Marie BirotJacques SamarutAnne MeyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 11, p e15507 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maïa Caillier Sandrine Thénot Violaine Tribollet Anne-Marie Birot Jacques Samarut Anne Mey Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.
description	The unique properties of embryonic stem cells (ESC) rely on long-lasting self-renewal and their ability to switch in all adult cell type programs. Recent advances have shown that regulations at the chromatin level sustain both ESC properties along with transcription factors. We have focused our interest on the epigenetic modulator HP1γ (Heterochromatin Protein 1, isoform γ) that binds histones H3 methylated at lysine 9 (meH3K9) and is highly plastic in its distribution and association with the transcriptional regulation of specific genes during cell fate transitions. These characteristics of HP1γ make it a good candidate to sustain the ESC flexibility required for rapid program changes during differentiation. Using RNA interference, we describe the functional role of HP1γ in mouse ESC. The analysis of HP1γ deprived cells in proliferative and in various differentiating conditions was performed combining functional assays with molecular approaches (RT-qPCR, microarray). We show that HP1γ deprivation slows down the cell cycle of ESC and decreases their resistance to differentiating conditions, rendering the cells poised to differentiate. In addition, HP1γ depletion hampers the differentiation to the endoderm as compared with the differentiation to the neurectoderm or the mesoderm. Altogether, our results reveal the role of HP1γ in ESC self-renewal and in the balance between the pluripotent and the differentiation programs.
format	article
author	Maïa Caillier Sandrine Thénot Violaine Tribollet Anne-Marie Birot Jacques Samarut Anne Mey
author_facet	Maïa Caillier Sandrine Thénot Violaine Tribollet Anne-Marie Birot Jacques Samarut Anne Mey
author_sort	Maïa Caillier
title	Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.
title_short	Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.
title_full	Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.
title_fullStr	Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.
title_full_unstemmed	Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.
title_sort	role of the epigenetic regulator hp1γ in the control of embryonic stem cell properties.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/6ac0bc781c21472caac1536188915d29
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Role of the epigenetic regulator HP1γ in the control of embryonic stem cell properties.

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