Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells

Oleanolic acid (OA) is widely distributed in food and medicinal plants, it reportedly exerts anti-inflammatory and anti-cancer effects. In this study, we investigated the effect of OA on human gastric cancer cells AGS in vitro and in vivo. The OA treatment significantly inhibited the AGS cell viabil...

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Autores principales:	Jae-Han Lee, Eun-Seon Yoo, So-Hee Han, Gi-Hwan Jung, Eun-Ji Han, Soo-Hyun Jung, Bum Seok Kim, Sung-Dae Cho, Jeong-Seok Nam, Changsun Choi, Jeong-Hwan Che, Ji-Youn Jung
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Oleanolic acid Apoptosis Autophagy PI3K/AKT/mTOR pathway Gastric cancer Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/6ac0df86965b49d8ab429c4b7b0e3e0f
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spelling	oai:doaj.org-article:6ac0df86965b49d8ab429c4b7b0e3e0f2021-11-12T04:29:09ZOleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells1756-464610.1016/j.jff.2021.104854https://doaj.org/article/6ac0df86965b49d8ab429c4b7b0e3e0f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S175646462100503Xhttps://doaj.org/toc/1756-4646Oleanolic acid (OA) is widely distributed in food and medicinal plants, it reportedly exerts anti-inflammatory and anti-cancer effects. In this study, we investigated the effect of OA on human gastric cancer cells AGS in vitro and in vivo. The OA treatment significantly inhibited the AGS cell viability. Apoptosis was confirmed via annexin V/PI staining and 4′,6-diamidino-2-phenylindole (DAPI) staining. The results from western blotting revealed that treatment with OA affected apoptosis-related protein. Meanwhile, OA induced autophagy, characterized by the formation of autophagic vacuoles and acidic vesicular organelles, also increased autophagy-related protein. Inhibition of autophagy further reduced cell proliferation. Moreover, OA treatment decreased phosphorylation of PI3K/AKT/mTOR pathway proteins. Finally, we found that OA reduced tumor volume and weight in xenograft mice via apoptosis without side effects. Overall, our study provides experimental evidence for the anti-cancer action of OA and suggests the possibility of its use as an adjuvant in gastric cancer therapy.Jae-Han LeeEun-Seon YooSo-Hee HanGi-Hwan JungEun-Ji HanSoo-Hyun JungBum Seok KimSung-Dae ChoJeong-Seok NamChangsun ChoiJeong-Hwan CheJi-Youn JungElsevierarticleOleanolic acidApoptosisAutophagyPI3K/AKT/mTOR pathwayGastric cancerNutrition. Foods and food supplyTX341-641ENJournal of Functional Foods, Vol 87, Iss , Pp 104854- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oleanolic acid Apoptosis Autophagy PI3K/AKT/mTOR pathway Gastric cancer Nutrition. Foods and food supply TX341-641
spellingShingle	Oleanolic acid Apoptosis Autophagy PI3K/AKT/mTOR pathway Gastric cancer Nutrition. Foods and food supply TX341-641 Jae-Han Lee Eun-Seon Yoo So-Hee Han Gi-Hwan Jung Eun-Ji Han Soo-Hyun Jung Bum Seok Kim Sung-Dae Cho Jeong-Seok Nam Changsun Choi Jeong-Hwan Che Ji-Youn Jung Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells
description	Oleanolic acid (OA) is widely distributed in food and medicinal plants, it reportedly exerts anti-inflammatory and anti-cancer effects. In this study, we investigated the effect of OA on human gastric cancer cells AGS in vitro and in vivo. The OA treatment significantly inhibited the AGS cell viability. Apoptosis was confirmed via annexin V/PI staining and 4′,6-diamidino-2-phenylindole (DAPI) staining. The results from western blotting revealed that treatment with OA affected apoptosis-related protein. Meanwhile, OA induced autophagy, characterized by the formation of autophagic vacuoles and acidic vesicular organelles, also increased autophagy-related protein. Inhibition of autophagy further reduced cell proliferation. Moreover, OA treatment decreased phosphorylation of PI3K/AKT/mTOR pathway proteins. Finally, we found that OA reduced tumor volume and weight in xenograft mice via apoptosis without side effects. Overall, our study provides experimental evidence for the anti-cancer action of OA and suggests the possibility of its use as an adjuvant in gastric cancer therapy.
format	article
author	Jae-Han Lee Eun-Seon Yoo So-Hee Han Gi-Hwan Jung Eun-Ji Han Soo-Hyun Jung Bum Seok Kim Sung-Dae Cho Jeong-Seok Nam Changsun Choi Jeong-Hwan Che Ji-Youn Jung
author_facet	Jae-Han Lee Eun-Seon Yoo So-Hee Han Gi-Hwan Jung Eun-Ji Han Soo-Hyun Jung Bum Seok Kim Sung-Dae Cho Jeong-Seok Nam Changsun Choi Jeong-Hwan Che Ji-Youn Jung
author_sort	Jae-Han Lee
title	Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells
title_short	Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells
title_full	Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells
title_fullStr	Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells
title_full_unstemmed	Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells
title_sort	oleanolic acid induces apoptosis and autophagy via the pi3k/akt/mtor pathway in ags human gastric cancer cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/6ac0df86965b49d8ab429c4b7b0e3e0f
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Oleanolic acid induces apoptosis and autophagy via the PI3K/AKT/mTOR pathway in AGS human gastric cancer cells

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