Extracellular detection of neuronal coupling

Abstract We developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple elect...

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Autores principales:	Elmer Guzman, Zhuowei Cheng, Paul K. Hansma, Kenneth R. Tovar, Linda R. Petzold, Kenneth S. Kosik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ac560e02a154a589e0c0676a99ca60d
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spelling	oai:doaj.org-article:6ac560e02a154a589e0c0676a99ca60d2021-12-02T17:55:13ZExtracellular detection of neuronal coupling10.1038/s41598-021-94282-62045-2322https://doaj.org/article/6ac560e02a154a589e0c0676a99ca60d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94282-6https://doaj.org/toc/2045-2322Abstract We developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple electrodes. Detecting eAP propagation bypasses ambiguity introduced by spike sorting. Our methods identify short latency spiking relationships between neurons with properties expected of synaptically coupled neurons, namely they were recapitulated by direct stimulation and were sensitive to changing the number of active synaptic sites. Our methods enabled us to assemble a functional subset of neuronal connectivity in our cultures.Elmer GuzmanZhuowei ChengPaul K. HansmaKenneth R. TovarLinda R. PetzoldKenneth S. KosikNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elmer Guzman Zhuowei Cheng Paul K. Hansma Kenneth R. Tovar Linda R. Petzold Kenneth S. Kosik Extracellular detection of neuronal coupling
description	Abstract We developed a method to non-invasively detect synaptic relationships among neurons from in vitro networks. Our method uses microelectrode arrays on which neurons are cultured and from which propagation of extracellular action potentials (eAPs) in single axons are recorded at multiple electrodes. Detecting eAP propagation bypasses ambiguity introduced by spike sorting. Our methods identify short latency spiking relationships between neurons with properties expected of synaptically coupled neurons, namely they were recapitulated by direct stimulation and were sensitive to changing the number of active synaptic sites. Our methods enabled us to assemble a functional subset of neuronal connectivity in our cultures.
format	article
author	Elmer Guzman Zhuowei Cheng Paul K. Hansma Kenneth R. Tovar Linda R. Petzold Kenneth S. Kosik
author_facet	Elmer Guzman Zhuowei Cheng Paul K. Hansma Kenneth R. Tovar Linda R. Petzold Kenneth S. Kosik
author_sort	Elmer Guzman
title	Extracellular detection of neuronal coupling
title_short	Extracellular detection of neuronal coupling
title_full	Extracellular detection of neuronal coupling
title_fullStr	Extracellular detection of neuronal coupling
title_full_unstemmed	Extracellular detection of neuronal coupling
title_sort	extracellular detection of neuronal coupling
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6ac560e02a154a589e0c0676a99ca60d
work_keys_str_mv	AT elmerguzman extracellulardetectionofneuronalcoupling AT zhuoweicheng extracellulardetectionofneuronalcoupling AT paulkhansma extracellulardetectionofneuronalcoupling AT kennethrtovar extracellulardetectionofneuronalcoupling AT lindarpetzold extracellulardetectionofneuronalcoupling AT kennethskosik extracellulardetectionofneuronalcoupling
_version_	1718379112392818688

Extracellular detection of neuronal coupling

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