<i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver

<i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver

As a chronic non-infectious disease, severely affecting human quality and health of life, diabetes mellitus (DM) and its complications have gradually developed into a major global public health problem. <i>Mulberry Leaf</i> and <i>Radix Astragali</i> have been used as a tradi...

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Autores principales: Shu Zhang, Qi Ge, Liang Chen, Keping Chen
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Mulberry Leaf</i>
<i>Radix Astragali</i>
transcriptomics and proteomics
diabetes mellitus
qRT-Pcr
western blot
Chemical technology
TP1-1185
Chemistry
QD1-999
Acceso en línea:https://doaj.org/article/6ac89a176583492f8088a6d9fc71a143
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spelling oai:doaj.org-article:6ac89a176583492f8088a6d9fc71a1432021-11-25T18:50:13Z<i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver10.3390/pr91118982227-9717https://doaj.org/article/6ac89a176583492f8088a6d9fc71a1432021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/1898https://doaj.org/toc/2227-9717As a chronic non-infectious disease, severely affecting human quality and health of life, diabetes mellitus (DM) and its complications have gradually developed into a major global public health problem. <i>Mulberry Leaf</i> and <i>Radix Astragali</i> have been used as a traditional medicinal formulation in diabetic patients for a long time, whose combination is usually found in traditional Chinese medicine prescriptions. However, due to the unclear synergistic mechanism of them for DM, the changes of differential genes and proteins in the liver tissue of streptozotocin-induced diabetic mice were analyzed, and then the potential synergistic mechanism of them in anti-diabetes was investigated in our research. Compared with the diabetic model group, there were 699 differentially expressed genes and 169 differentially expressed proteins in the <i>Mulberry Leaf</i> and <i>Radix Astragali</i> treated group, and there were 35 common specific genes both in the transcriptome and the proteome. These common genes participated mainly in the pathways, such as retinol metabolism, steroid hormone biosynthesis, and arachidonic acid metabolism. Quantitative real-time PCR() and Western blot results speculated that the synergistic effect on anti-diabetes was mainly through regulating the expression of Tap1, Ncoa4, and Alas2, by down-regulating Fabp2 and Hmox1 and up-regulating Hmgcr, Cyp7a1. All these genes would affect bile acid secretion, alleviate the occurrence of iron death, promote the metabolism and synthesis of glycolipid substances, and ultimately maintain the body’s glucose homeostasis.Shu ZhangQi GeLiang ChenKeping ChenMDPI AGarticle<i>Mulberry Leaf</i><i>Radix Astragali</i>transcriptomics and proteomicsdiabetes mellitusqRT-Pcrwestern blotChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 1898, p 1898 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Mulberry Leaf</i>
<i>Radix Astragali</i>
transcriptomics and proteomics
diabetes mellitus
qRT-Pcr
western blot
Chemical technology
TP1-1185
Chemistry
QD1-999
spellingShingle <i>Mulberry Leaf</i>
<i>Radix Astragali</i>
transcriptomics and proteomics
diabetes mellitus
qRT-Pcr
western blot
Chemical technology
TP1-1185
Chemistry
QD1-999
Shu Zhang
Qi Ge
Liang Chen
Keping Chen
<i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver
description As a chronic non-infectious disease, severely affecting human quality and health of life, diabetes mellitus (DM) and its complications have gradually developed into a major global public health problem. <i>Mulberry Leaf</i> and <i>Radix Astragali</i> have been used as a traditional medicinal formulation in diabetic patients for a long time, whose combination is usually found in traditional Chinese medicine prescriptions. However, due to the unclear synergistic mechanism of them for DM, the changes of differential genes and proteins in the liver tissue of streptozotocin-induced diabetic mice were analyzed, and then the potential synergistic mechanism of them in anti-diabetes was investigated in our research. Compared with the diabetic model group, there were 699 differentially expressed genes and 169 differentially expressed proteins in the <i>Mulberry Leaf</i> and <i>Radix Astragali</i> treated group, and there were 35 common specific genes both in the transcriptome and the proteome. These common genes participated mainly in the pathways, such as retinol metabolism, steroid hormone biosynthesis, and arachidonic acid metabolism. Quantitative real-time PCR() and Western blot results speculated that the synergistic effect on anti-diabetes was mainly through regulating the expression of Tap1, Ncoa4, and Alas2, by down-regulating Fabp2 and Hmox1 and up-regulating Hmgcr, Cyp7a1. All these genes would affect bile acid secretion, alleviate the occurrence of iron death, promote the metabolism and synthesis of glycolipid substances, and ultimately maintain the body’s glucose homeostasis.
format article
author Shu Zhang
Qi Ge
Liang Chen
Keping Chen
author_facet Shu Zhang
Qi Ge
Liang Chen
Keping Chen
author_sort Shu Zhang
title <i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver
title_short <i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver
title_full <i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver
title_fullStr <i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver
title_full_unstemmed <i>Mulberry Leaf</i> and <i>Radix Astragali</i> Regulates Differentially Expressed Genes and Proteins in the Streptozotocin-Induced Diabetic Mice Liver
title_sort <i>mulberry leaf</i> and <i>radix astragali</i> regulates differentially expressed genes and proteins in the streptozotocin-induced diabetic mice liver
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/6ac89a176583492f8088a6d9fc71a143
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AT liangchen imulberryleafiandiradixastragaliiregulatesdifferentiallyexpressedgenesandproteinsinthestreptozotocininduceddiabeticmiceliver
AT kepingchen imulberryleafiandiradixastragaliiregulatesdifferentiallyexpressedgenesandproteinsinthestreptozotocininduceddiabeticmiceliver
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