REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS

The levels of TLR/RLR gene expression and production of some cytokines were studied in monocytic THP-1 cell line during its differentiation to mature macrophage-like forms induced by phorbol 12-myristate 13-acetate (PMA) treatment for 1 and 5 days in vitro. For the first time, we have shown high ind...

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Autores principales:	T. M. Sokolova, V. V. Poloskov, A. N. Shuvalov, F. I. Ershov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2017
Materias:	thp-1 cells pma differentiation macrophages tlr/rlr genes cytokines tlr-agonists Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/6ad216fcd0e5447faba9111fad76b351
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spelling	oai:doaj.org-article:6ad216fcd0e5447faba9111fad76b3512021-11-18T08:03:45ZREGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS1563-06252313-741X10.15789/1563-0625-2017-1-27-34https://doaj.org/article/6ad216fcd0e5447faba9111fad76b3512017-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1162https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe levels of TLR/RLR gene expression and production of some cytokines were studied in monocytic THP-1 cell line during its differentiation to mature macrophage-like forms induced by phorbol 12-myristate 13-acetate (PMA) treatment for 1 and 5 days in vitro. For the first time, we have shown high induction levels for the genes that encode signaling immune receptors and transcription factors in response to PMA, as well as inhibitory effects of TLR3, TLR7/TLR8, TLR9-agonists in mature macrophages. The PMAactivated THP-1 macrophage-like cells secreted large quantitities of inflammatory IL-1β and TNFα cytokines into culture medium.T. M. SokolovaV. V. PoloskovA. N. ShuvalovF. I. ErshovSPb RAACIarticlethp-1 cellspmadifferentiationmacrophagestlr/rlr genescytokinestlr-agonistsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 19, Iss 1, Pp 27-34 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	thp-1 cells pma differentiation macrophages tlr/rlr genes cytokines tlr-agonists Immunologic diseases. Allergy RC581-607
spellingShingle	thp-1 cells pma differentiation macrophages tlr/rlr genes cytokines tlr-agonists Immunologic diseases. Allergy RC581-607 T. M. Sokolova V. V. Poloskov A. N. Shuvalov F. I. Ershov REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS
description	The levels of TLR/RLR gene expression and production of some cytokines were studied in monocytic THP-1 cell line during its differentiation to mature macrophage-like forms induced by phorbol 12-myristate 13-acetate (PMA) treatment for 1 and 5 days in vitro. For the first time, we have shown high induction levels for the genes that encode signaling immune receptors and transcription factors in response to PMA, as well as inhibitory effects of TLR3, TLR7/TLR8, TLR9-agonists in mature macrophages. The PMAactivated THP-1 macrophage-like cells secreted large quantitities of inflammatory IL-1β and TNFα cytokines into culture medium.
format	article
author	T. M. Sokolova V. V. Poloskov A. N. Shuvalov F. I. Ershov
author_facet	T. M. Sokolova V. V. Poloskov A. N. Shuvalov F. I. Ershov
author_sort	T. M. Sokolova
title	REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS
title_short	REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS
title_full	REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS
title_fullStr	REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS
title_full_unstemmed	REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS
title_sort	regulation of tlr/rlr gene activity and synthesis of cytokines during phorbol myristate acetate (pma)-induced differentiation of thp-1 monocytes into macrophage-like cells
publisher	SPb RAACI
publishDate	2017
url	https://doaj.org/article/6ad216fcd0e5447faba9111fad76b351
work_keys_str_mv	AT tmsokolova regulationoftlrrlrgeneactivityandsynthesisofcytokinesduringphorbolmyristateacetatepmainduceddifferentiationofthp1monocytesintomacrophagelikecells AT vvpoloskov regulationoftlrrlrgeneactivityandsynthesisofcytokinesduringphorbolmyristateacetatepmainduceddifferentiationofthp1monocytesintomacrophagelikecells AT anshuvalov regulationoftlrrlrgeneactivityandsynthesisofcytokinesduringphorbolmyristateacetatepmainduceddifferentiationofthp1monocytesintomacrophagelikecells AT fiershov regulationoftlrrlrgeneactivityandsynthesisofcytokinesduringphorbolmyristateacetatepmainduceddifferentiationofthp1monocytesintomacrophagelikecells
_version_	1718422423287627776

REGULATION OF TLR/RLR GENE ACTIVITY AND SYNTHESIS OF CYTOKINES DURING PHORBOL MYRISTATE ACETATE (PMA)-INDUCED DIFFERENTIATION OF THP-1 MONOCYTES INTO MACROPHAGE-LIKE CELLS

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