Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules

Achieving high axial resolution is challenging in single-molecule localization microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic n...

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Autores principales:	Alan M. Szalai, Bruno Siarry, Jerónimo Lukin, David J. Williamson, Nicolás Unsain, Alfredo Cáceres, Mauricio Pilo-Pais, Guillermo Acuna, Damián Refojo, Dylan M. Owen, Sabrina Simoncelli, Fernando D. Stefani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/6ad498979d8b41b29e474f3f28d8f096
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spelling	oai:doaj.org-article:6ad498979d8b41b29e474f3f28d8f0962021-12-02T14:19:18ZThree-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules10.1038/s41467-020-20863-02041-1723https://doaj.org/article/6ad498979d8b41b29e474f3f28d8f0962021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20863-0https://doaj.org/toc/2041-1723Achieving high axial resolution is challenging in single-molecule localization microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic nanometric resolution.Alan M. SzalaiBruno SiarryJerónimo LukinDavid J. WilliamsonNicolás UnsainAlfredo CáceresMauricio Pilo-PaisGuillermo AcunaDamián RefojoDylan M. OwenSabrina SimoncelliFernando D. StefaniNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alan M. Szalai Bruno Siarry Jerónimo Lukin David J. Williamson Nicolás Unsain Alfredo Cáceres Mauricio Pilo-Pais Guillermo Acuna Damián Refojo Dylan M. Owen Sabrina Simoncelli Fernando D. Stefani Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
description	Achieving high axial resolution is challenging in single-molecule localization microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic nanometric resolution.
format	article
author	Alan M. Szalai Bruno Siarry Jerónimo Lukin David J. Williamson Nicolás Unsain Alfredo Cáceres Mauricio Pilo-Pais Guillermo Acuna Damián Refojo Dylan M. Owen Sabrina Simoncelli Fernando D. Stefani
author_facet	Alan M. Szalai Bruno Siarry Jerónimo Lukin David J. Williamson Nicolás Unsain Alfredo Cáceres Mauricio Pilo-Pais Guillermo Acuna Damián Refojo Dylan M. Owen Sabrina Simoncelli Fernando D. Stefani
author_sort	Alan M. Szalai
title	Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
title_short	Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
title_full	Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
title_fullStr	Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
title_full_unstemmed	Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
title_sort	three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6ad498979d8b41b29e474f3f28d8f096
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Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules

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