Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)

Abstract Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases resp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xue Liu, Hucheng Jiang, Baoqing Ye, Hongli Qian, Ziqi Guo, Haotian Bai, Jinhua Gong, Jianbin Feng, Keyi Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ad67e7fa7604445a9b30a7627d91f26
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6ad67e7fa7604445a9b30a7627d91f26
record_format	dspace
spelling	oai:doaj.org-article:6ad67e7fa7604445a9b30a7627d91f262021-12-02T19:06:40ZComparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)10.1038/s41598-021-95709-w2045-2322https://doaj.org/article/6ad67e7fa7604445a9b30a7627d91f262021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95709-whttps://doaj.org/toc/2045-2322Abstract Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd2+ stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd2+ exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd2+ treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd2+ exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd2+ stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd2+. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd2+ stress in M. rosenbergii, which lays the foundation for further understanding of heavy metal stress.Xue LiuHucheng JiangBaoqing YeHongli QianZiqi GuoHaotian BaiJinhua GongJianbin FengKeyi MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xue Liu Hucheng Jiang Baoqing Ye Hongli Qian Ziqi Guo Haotian Bai Jinhua Gong Jianbin Feng Keyi Ma Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)
description	Abstract Heavy metal Cadmium (Cd2+) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd2+ stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd2+ exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd2+ treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd2+ exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd2+ stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd2+. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd2+ stress in M. rosenbergii, which lays the foundation for further understanding of heavy metal stress.
format	article
author	Xue Liu Hucheng Jiang Baoqing Ye Hongli Qian Ziqi Guo Haotian Bai Jinhua Gong Jianbin Feng Keyi Ma
author_facet	Xue Liu Hucheng Jiang Baoqing Ye Hongli Qian Ziqi Guo Haotian Bai Jinhua Gong Jianbin Feng Keyi Ma
author_sort	Xue Liu
title	Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)
title_short	Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)
title_full	Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)
title_fullStr	Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)
title_full_unstemmed	Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)
title_sort	comparative transcriptome analysis of the gills and hepatopancreas from macrobrachium rosenbergii exposed to the heavy metal cadmium (cd2+)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6ad67e7fa7604445a9b30a7627d91f26
work_keys_str_mv	AT xueliu comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT huchengjiang comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT baoqingye comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT hongliqian comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT ziqiguo comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT haotianbai comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT jinhuagong comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT jianbinfeng comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2 AT keyima comparativetranscriptomeanalysisofthegillsandhepatopancreasfrommacrobrachiumrosenbergiiexposedtotheheavymetalcadmiumcd2
_version_	1718377155104079872

Comparative transcriptome analysis of the gills and hepatopancreas from Macrobrachium rosenbergii exposed to the heavy metal Cadmium (Cd2+)

Ejemplares similares