Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.

Gestational exposure to environmental toxins such as nicotine may result in detectable gene expression changes in later life. To investigate the direct toxic effects of prenatal nicotine exposure on later brain development, we have used transcriptomic analysis of striatal samples to identify gene ex...

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Autores principales:	Nicholas E Ilott, Tomasz Schneider, Jonathan Mill, Leonard Schalkwyk, Giovana Brolese, Lisiane Bizarro, Ian P Stolerman, Emma Dempster, Philip Asherson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6adee7273bc34f2f94484d0d4d13452f
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spelling	oai:doaj.org-article:6adee7273bc34f2f94484d0d4d13452f2021-11-18T08:32:02ZLong-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.1932-620310.1371/journal.pone.0088896https://doaj.org/article/6adee7273bc34f2f94484d0d4d13452f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586432/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Gestational exposure to environmental toxins such as nicotine may result in detectable gene expression changes in later life. To investigate the direct toxic effects of prenatal nicotine exposure on later brain development, we have used transcriptomic analysis of striatal samples to identify gene expression differences between adolescent Lister Hooded rats exposed to nicotine in utero and controls. Using an additional group of animals matched for the reduced food intake experienced in the nicotine group, we were also able to assess the impact of imposed food-restriction on gene expression profiles. We found little evidence for a role of gestational nicotine exposure on altered gene expression in the striatum of adolescent offspring at a significance level of p<0.01 and \|log2 fold change >0.5\|, although we cannot exclude the possibility of nicotine-induced changes in other brain regions, or at other time points. We did, however, find marked gene expression differences in response to imposed food-restriction. Food-restriction resulted in significant group differences for a number of immediate early genes (IEGs) including Fos, Fosb, Fosl2, Arc, Junb, Nr4a1 and Nr4a3. These genes are associated with stress response pathways and therefore may reflect long-term effects of nutritional deprivation on the development of the stress system.Nicholas E IlottTomasz SchneiderJonathan MillLeonard SchalkwykGiovana BroleseLisiane BizarroIan P StolermanEmma DempsterPhilip AshersonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88896 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicholas E Ilott Tomasz Schneider Jonathan Mill Leonard Schalkwyk Giovana Brolese Lisiane Bizarro Ian P Stolerman Emma Dempster Philip Asherson Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.
description	Gestational exposure to environmental toxins such as nicotine may result in detectable gene expression changes in later life. To investigate the direct toxic effects of prenatal nicotine exposure on later brain development, we have used transcriptomic analysis of striatal samples to identify gene expression differences between adolescent Lister Hooded rats exposed to nicotine in utero and controls. Using an additional group of animals matched for the reduced food intake experienced in the nicotine group, we were also able to assess the impact of imposed food-restriction on gene expression profiles. We found little evidence for a role of gestational nicotine exposure on altered gene expression in the striatum of adolescent offspring at a significance level of p<0.01 and \|log2 fold change >0.5\|, although we cannot exclude the possibility of nicotine-induced changes in other brain regions, or at other time points. We did, however, find marked gene expression differences in response to imposed food-restriction. Food-restriction resulted in significant group differences for a number of immediate early genes (IEGs) including Fos, Fosb, Fosl2, Arc, Junb, Nr4a1 and Nr4a3. These genes are associated with stress response pathways and therefore may reflect long-term effects of nutritional deprivation on the development of the stress system.
format	article
author	Nicholas E Ilott Tomasz Schneider Jonathan Mill Leonard Schalkwyk Giovana Brolese Lisiane Bizarro Ian P Stolerman Emma Dempster Philip Asherson
author_facet	Nicholas E Ilott Tomasz Schneider Jonathan Mill Leonard Schalkwyk Giovana Brolese Lisiane Bizarro Ian P Stolerman Emma Dempster Philip Asherson
author_sort	Nicholas E Ilott
title	Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.
title_short	Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.
title_full	Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.
title_fullStr	Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.
title_full_unstemmed	Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.
title_sort	long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/6adee7273bc34f2f94484d0d4d13452f
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Long-term effects of gestational nicotine exposure and food-restriction on gene expression in the striatum of adolescent rats.

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