Relationship of C5L2 receptor to skeletal muscle substrate utilization.

<h4>Objective</h4>To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.<h4>Design and methods</h4>...

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Autores principales:	Christian Roy, Sabina Paglialunga, Gert Schaart, Esther Moonen-Kornips, Ruth C Meex, Esther Phielix, Joris Hoeks, Matthijs K C Hesselink, Katherine Cianflone, Patrick Schrauwen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ae46696de1d449fbb497be7e2226bd8
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spelling	oai:doaj.org-article:6ae46696de1d449fbb497be7e2226bd82021-11-18T07:55:47ZRelationship of C5L2 receptor to skeletal muscle substrate utilization.1932-620310.1371/journal.pone.0057494https://doaj.org/article/6ae46696de1d449fbb497be7e2226bd82013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23460866/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.<h4>Design and methods</h4>Male wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.<h4>Results</h4>IMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (p<0.05) and plasma insulin levels were markedly increased in C5L2KO-HFD mice (twofold, p<0.05). Muscle HADH activity was elevated in C5L2KO-LFD mice (+75%, p<0.001 vs. WT-LFD) and C5L2KO-HFD displayed increased mitochondrial fatty acid oxidative capacity compared to WT-HFD mice (+23%, p<0.05). In human subjects, C5L2 protein content was reduced (-48%, p<0.01) in type 2 diabetic patients when compared to obese controls. Further, exercise training increased C5L2 (+45%, p = 0.0019) and ASP (+80%, p<0.001) in obese insulin-resistant men.<h4>Conclusion</h4>The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.Christian RoySabina PaglialungaGert SchaartEsther Moonen-KornipsRuth C MeexEsther PhielixJoris HoeksMatthijs K C HesselinkKatherine CianflonePatrick SchrauwenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e57494 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Roy Sabina Paglialunga Gert Schaart Esther Moonen-Kornips Ruth C Meex Esther Phielix Joris Hoeks Matthijs K C Hesselink Katherine Cianflone Patrick Schrauwen Relationship of C5L2 receptor to skeletal muscle substrate utilization.
description	<h4>Objective</h4>To investigate the role of Acylation Stimulating Protein (ASP) receptor C5L2 in skeletal muscle fatty acid accumulation and metabolism as well as insulin sensitivity in both mice and human models of diet-induced insulin resistance.<h4>Design and methods</h4>Male wildtype (WT) and C5L2 knockout (KO) mice were fed a low (LFD) or a high (HFD) fat diet for 10 weeks. Intramyocellular lipid (IMCL) accumulation (by oil red O staining) and beta-oxidation HADH enzyme activity were determined in skeletal muscle. Mitochondria were isolated from hindleg muscles for high-resolution respirometry. Muscle C5L2 protein content was also determined in obese type 2 diabetics and age- and BMI matched men.<h4>Results</h4>IMCL levels were increased by six-fold in C5L2KO-HFD compared to WT-HFD mice (p<0.05) and plasma insulin levels were markedly increased in C5L2KO-HFD mice (twofold, p<0.05). Muscle HADH activity was elevated in C5L2KO-LFD mice (+75%, p<0.001 vs. WT-LFD) and C5L2KO-HFD displayed increased mitochondrial fatty acid oxidative capacity compared to WT-HFD mice (+23%, p<0.05). In human subjects, C5L2 protein content was reduced (-48%, p<0.01) in type 2 diabetic patients when compared to obese controls. Further, exercise training increased C5L2 (+45%, p = 0.0019) and ASP (+80%, p<0.001) in obese insulin-resistant men.<h4>Conclusion</h4>The results suggest that insulin sensitivity may be permissive for coupling of C5L2 levels to lipid storage and utilization.
format	article
author	Christian Roy Sabina Paglialunga Gert Schaart Esther Moonen-Kornips Ruth C Meex Esther Phielix Joris Hoeks Matthijs K C Hesselink Katherine Cianflone Patrick Schrauwen
author_facet	Christian Roy Sabina Paglialunga Gert Schaart Esther Moonen-Kornips Ruth C Meex Esther Phielix Joris Hoeks Matthijs K C Hesselink Katherine Cianflone Patrick Schrauwen
author_sort	Christian Roy
title	Relationship of C5L2 receptor to skeletal muscle substrate utilization.
title_short	Relationship of C5L2 receptor to skeletal muscle substrate utilization.
title_full	Relationship of C5L2 receptor to skeletal muscle substrate utilization.
title_fullStr	Relationship of C5L2 receptor to skeletal muscle substrate utilization.
title_full_unstemmed	Relationship of C5L2 receptor to skeletal muscle substrate utilization.
title_sort	relationship of c5l2 receptor to skeletal muscle substrate utilization.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/6ae46696de1d449fbb497be7e2226bd8
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_version_	1718422735884910592

Relationship of C5L2 receptor to skeletal muscle substrate utilization.

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