New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization

Abstract The advanced biomimetic mineralization technology was applied to protect the Botulinum neurotoxin type D, and the processing of the mineralization granule of botulinum toxin type D was successfully screened. The loss of activity of the toxin protein at different temperatures and the destruc...

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Autores principales:	Shengqing Li, Xiyun Zhang, Guoyuan Hu, Shuping Li, Zhining Li, Yuxia Fan, Yanming Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ae62ac36e24436d9705027751c1dd95
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spelling	oai:doaj.org-article:6ae62ac36e24436d9705027751c1dd952021-12-02T12:11:34ZNew technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization10.1038/s41598-021-83733-92045-2322https://doaj.org/article/6ae62ac36e24436d9705027751c1dd952021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83733-9https://doaj.org/toc/2045-2322Abstract The advanced biomimetic mineralization technology was applied to protect the Botulinum neurotoxin type D, and the processing of the mineralization granule of botulinum toxin type D was successfully screened. The loss of activity of the toxin protein at different temperatures and the destructive strength of the gastrointestinal tract against the toxin were determined biologically. The lethal toxicity of the mineralized toxin to wild rodents was determined by median lethal dose. Protective tests at different temperatures showed that the preservation period of botulinum toxin type D mineralized sample 2 was significantly higher than that of the control group at three different temperatures, and its toxicity loss was significantly reduced. The damage intensity of the mineralized toxin to the gastrointestinal contents of plateau zokor and plateau pika was significantly reduced. The minimum lethal doses of the mineralized toxin particles to plateau zokor, plateau pika, and mice were 5200, 8,600,000, and 25,000 MLD/kg. These results showed that biomimetic mineralization could greatly improve the thermal stability of botulinum toxin type D and reduce the damaging effect of the gastrointestinal contents of target animals to botulinum toxin type D. The mineralized toxin could be used to control the population density of urban rodents. This research provides new insights into the protection of toxin protein substances.Shengqing LiXiyun ZhangGuoyuan HuShuping LiZhining LiYuxia FanYanming ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shengqing Li Xiyun Zhang Guoyuan Hu Shuping Li Zhining Li Yuxia Fan Yanming Zhang New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization
description	Abstract The advanced biomimetic mineralization technology was applied to protect the Botulinum neurotoxin type D, and the processing of the mineralization granule of botulinum toxin type D was successfully screened. The loss of activity of the toxin protein at different temperatures and the destructive strength of the gastrointestinal tract against the toxin were determined biologically. The lethal toxicity of the mineralized toxin to wild rodents was determined by median lethal dose. Protective tests at different temperatures showed that the preservation period of botulinum toxin type D mineralized sample 2 was significantly higher than that of the control group at three different temperatures, and its toxicity loss was significantly reduced. The damage intensity of the mineralized toxin to the gastrointestinal contents of plateau zokor and plateau pika was significantly reduced. The minimum lethal doses of the mineralized toxin particles to plateau zokor, plateau pika, and mice were 5200, 8,600,000, and 25,000 MLD/kg. These results showed that biomimetic mineralization could greatly improve the thermal stability of botulinum toxin type D and reduce the damaging effect of the gastrointestinal contents of target animals to botulinum toxin type D. The mineralized toxin could be used to control the population density of urban rodents. This research provides new insights into the protection of toxin protein substances.
format	article
author	Shengqing Li Xiyun Zhang Guoyuan Hu Shuping Li Zhining Li Yuxia Fan Yanming Zhang
author_facet	Shengqing Li Xiyun Zhang Guoyuan Hu Shuping Li Zhining Li Yuxia Fan Yanming Zhang
author_sort	Shengqing Li
title	New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization
title_short	New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization
title_full	New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization
title_fullStr	New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization
title_full_unstemmed	New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization
title_sort	new technology to improve the thermal stability of botulinum toxin type d by biomimetic mineralization
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6ae62ac36e24436d9705027751c1dd95
work_keys_str_mv	AT shengqingli newtechnologytoimprovethethermalstabilityofbotulinumtoxintypedbybiomimeticmineralization AT xiyunzhang newtechnologytoimprovethethermalstabilityofbotulinumtoxintypedbybiomimeticmineralization AT guoyuanhu newtechnologytoimprovethethermalstabilityofbotulinumtoxintypedbybiomimeticmineralization AT shupingli newtechnologytoimprovethethermalstabilityofbotulinumtoxintypedbybiomimeticmineralization AT zhiningli newtechnologytoimprovethethermalstabilityofbotulinumtoxintypedbybiomimeticmineralization AT yuxiafan newtechnologytoimprovethethermalstabilityofbotulinumtoxintypedbybiomimeticmineralization AT yanmingzhang newtechnologytoimprovethethermalstabilityofbotulinumtoxintypedbybiomimeticmineralization
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New technology to improve the thermal stability of botulinum toxin type D by biomimetic mineralization

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