High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM

Abstract In vitro gliding assay of microtubules (MTs) on kinesins has provided us with valuable biophysical and chemo-mechanical insights of this biomolecular motor system. Visualization of MTs in an in vitro gliding assay has been mainly dependent on optical microscopes, limited resolution of which...

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Autores principales:	Jakia Jannat Keya, Daisuke Inoue, Yuki Suzuki, Toshiya Kozai, Daiki Ishikuro, Noriyuki Kodera, Takayuki Uchihashi, Arif Md. Rashedul Kabir, Masayuki Endo, Kazuki Sada, Akira Kakugo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6ae72286804f47a8a2a1c2e5b12039e5
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spelling	oai:doaj.org-article:6ae72286804f47a8a2a1c2e5b12039e52021-12-02T16:06:31ZHigh-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM10.1038/s41598-017-06249-12045-2322https://doaj.org/article/6ae72286804f47a8a2a1c2e5b12039e52017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06249-1https://doaj.org/toc/2045-2322Abstract In vitro gliding assay of microtubules (MTs) on kinesins has provided us with valuable biophysical and chemo-mechanical insights of this biomolecular motor system. Visualization of MTs in an in vitro gliding assay has been mainly dependent on optical microscopes, limited resolution of which often render them insufficient sources of desired information. In this work, using high speed atomic force microscopy (HS-AFM), which allows imaging with higher resolution, we monitored MTs and protofilaments (PFs) of tubulins while gliding on kinesins. Moreover, under the HS-AFM, we also observed splitting of gliding MTs into single PFs at their leading ends. The split single PFs interacted with kinesins and exhibited translational motion, but with a slower velocity than the MTs. Our investigation at the molecular level, using the HS-AFM, would provide new insights to the mechanics of MTs in dynamic systems and their interaction with motor proteins.Jakia Jannat KeyaDaisuke InoueYuki SuzukiToshiya KozaiDaiki IshikuroNoriyuki KoderaTakayuki UchihashiArif Md. Rashedul KabirMasayuki EndoKazuki SadaAkira KakugoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jakia Jannat Keya Daisuke Inoue Yuki Suzuki Toshiya Kozai Daiki Ishikuro Noriyuki Kodera Takayuki Uchihashi Arif Md. Rashedul Kabir Masayuki Endo Kazuki Sada Akira Kakugo High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
description	Abstract In vitro gliding assay of microtubules (MTs) on kinesins has provided us with valuable biophysical and chemo-mechanical insights of this biomolecular motor system. Visualization of MTs in an in vitro gliding assay has been mainly dependent on optical microscopes, limited resolution of which often render them insufficient sources of desired information. In this work, using high speed atomic force microscopy (HS-AFM), which allows imaging with higher resolution, we monitored MTs and protofilaments (PFs) of tubulins while gliding on kinesins. Moreover, under the HS-AFM, we also observed splitting of gliding MTs into single PFs at their leading ends. The split single PFs interacted with kinesins and exhibited translational motion, but with a slower velocity than the MTs. Our investigation at the molecular level, using the HS-AFM, would provide new insights to the mechanics of MTs in dynamic systems and their interaction with motor proteins.
format	article
author	Jakia Jannat Keya Daisuke Inoue Yuki Suzuki Toshiya Kozai Daiki Ishikuro Noriyuki Kodera Takayuki Uchihashi Arif Md. Rashedul Kabir Masayuki Endo Kazuki Sada Akira Kakugo
author_facet	Jakia Jannat Keya Daisuke Inoue Yuki Suzuki Toshiya Kozai Daiki Ishikuro Noriyuki Kodera Takayuki Uchihashi Arif Md. Rashedul Kabir Masayuki Endo Kazuki Sada Akira Kakugo
author_sort	Jakia Jannat Keya
title	High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
title_short	High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
title_full	High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
title_fullStr	High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
title_full_unstemmed	High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
title_sort	high-resolution imaging of a single gliding protofilament of tubulins by hs-afm
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6ae72286804f47a8a2a1c2e5b12039e5
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High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM

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