Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>

ABSTRACT Fungi can use a wide variety of nitrogen sources. In the absence of preferred sources such as ammonium, glutamate, and glutamine, secondary sources, including most other amino acids, are used. Expression of the nitrogen utilization pathways is very strongly controlled at the transcriptional...

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Autores principales:	Siobhan A. Turner, Qinxi Ma, Mihaela Ola, Kontxi Martinez de San Vicente, Geraldine Butler
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Candida nitrogen metabolism opportunistic fungi Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/6aeb0f7434b34050bc0e75450e02d83b
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spelling	oai:doaj.org-article:6aeb0f7434b34050bc0e75450e02d83b2021-11-15T15:22:14ZDal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>10.1128/mSphere.00028-182379-5042https://doaj.org/article/6aeb0f7434b34050bc0e75450e02d83b2018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00028-18https://doaj.org/toc/2379-5042ABSTRACT Fungi can use a wide variety of nitrogen sources. In the absence of preferred sources such as ammonium, glutamate, and glutamine, secondary sources, including most other amino acids, are used. Expression of the nitrogen utilization pathways is very strongly controlled at the transcriptional level. Here, we investigated the regulation of nitrogen utilization in the pathogenic yeast Candida parapsilosis. We found that the functions of many regulators are conserved with respect to Saccharomyces cerevisiae and other fungi. For example, the core GATA activators GAT1 and GLN3 have a conserved role in nitrogen catabolite repression (NCR). There is one ortholog of GZF3 and DAL80, which represses expression of genes in preferred nitrogen sources. The regulators PUT3 and UGA3 are required for metabolism of proline and γ-aminobutyric acid (GABA), respectively. However, the role of the Dal81 transcription factor is distinctly different. In S. cerevisiae, Dal81 is a positive regulator of acquisition of nitrogen from GABA, allantoin, urea, and leucine, and it is required for maximal induction of expression of the relevant pathway genes. In C. parapsilosis, induction of GABA genes is independent of Dal81, and deleting DAL81 has no effect on acquisition of nitrogen from GABA or allantoin. Instead, Dal81 represses arginine synthesis during growth under preferred nitrogen conditions. IMPORTANCE Utilization of nitrogen by fungi is controlled by nitrogen catabolite repression (NCR). Expression of many genes is switched off during growth on nonpreferred nitrogen sources. Gene expression is regulated through a combination of activation and repression. Nitrogen regulation has been studied best in the model yeast Saccharomyces cerevisiae. We found that although many nitrogen regulators have a conserved function in Saccharomyces species, some do not. The Dal81 transcriptional regulator has distinctly different functions in S. cerevisiae and C. parapsilosis. In the former, it regulates utilization of nitrogen from GABA and allantoin, whereas in the latter, it regulates expression of arginine synthesis genes. Our findings make an important contribution to our understanding of nitrogen regulation in a human-pathogenic fungus.Siobhan A. TurnerQinxi MaMihaela OlaKontxi Martinez de San VicenteGeraldine ButlerAmerican Society for MicrobiologyarticleCandidanitrogen metabolismopportunistic fungiMicrobiologyQR1-502ENmSphere, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida nitrogen metabolism opportunistic fungi Microbiology QR1-502
spellingShingle	Candida nitrogen metabolism opportunistic fungi Microbiology QR1-502 Siobhan A. Turner Qinxi Ma Mihaela Ola Kontxi Martinez de San Vicente Geraldine Butler Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>
description	ABSTRACT Fungi can use a wide variety of nitrogen sources. In the absence of preferred sources such as ammonium, glutamate, and glutamine, secondary sources, including most other amino acids, are used. Expression of the nitrogen utilization pathways is very strongly controlled at the transcriptional level. Here, we investigated the regulation of nitrogen utilization in the pathogenic yeast Candida parapsilosis. We found that the functions of many regulators are conserved with respect to Saccharomyces cerevisiae and other fungi. For example, the core GATA activators GAT1 and GLN3 have a conserved role in nitrogen catabolite repression (NCR). There is one ortholog of GZF3 and DAL80, which represses expression of genes in preferred nitrogen sources. The regulators PUT3 and UGA3 are required for metabolism of proline and γ-aminobutyric acid (GABA), respectively. However, the role of the Dal81 transcription factor is distinctly different. In S. cerevisiae, Dal81 is a positive regulator of acquisition of nitrogen from GABA, allantoin, urea, and leucine, and it is required for maximal induction of expression of the relevant pathway genes. In C. parapsilosis, induction of GABA genes is independent of Dal81, and deleting DAL81 has no effect on acquisition of nitrogen from GABA or allantoin. Instead, Dal81 represses arginine synthesis during growth under preferred nitrogen conditions. IMPORTANCE Utilization of nitrogen by fungi is controlled by nitrogen catabolite repression (NCR). Expression of many genes is switched off during growth on nonpreferred nitrogen sources. Gene expression is regulated through a combination of activation and repression. Nitrogen regulation has been studied best in the model yeast Saccharomyces cerevisiae. We found that although many nitrogen regulators have a conserved function in Saccharomyces species, some do not. The Dal81 transcriptional regulator has distinctly different functions in S. cerevisiae and C. parapsilosis. In the former, it regulates utilization of nitrogen from GABA and allantoin, whereas in the latter, it regulates expression of arginine synthesis genes. Our findings make an important contribution to our understanding of nitrogen regulation in a human-pathogenic fungus.
format	article
author	Siobhan A. Turner Qinxi Ma Mihaela Ola Kontxi Martinez de San Vicente Geraldine Butler
author_facet	Siobhan A. Turner Qinxi Ma Mihaela Ola Kontxi Martinez de San Vicente Geraldine Butler
author_sort	Siobhan A. Turner
title	Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>
title_short	Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>
title_full	Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>
title_fullStr	Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>
title_full_unstemmed	Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>
title_sort	dal81 regulates expression of arginine metabolism genes in <named-content content-type="genus-species">candida parapsilosis</named-content>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/6aeb0f7434b34050bc0e75450e02d83b
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Dal81 Regulates Expression of Arginine Metabolism Genes in <named-content content-type="genus-species">Candida parapsilosis</named-content>

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