Features of local immunity in women with endometriosis and genital infection

Introduction. External genital endometriosis is an inflammatory estrogen-dependent disease characterized by implantation and proliferation of endometrial tissue outside the uterus, accompanied by increased production of proinflammatory cytokines, prostaglandins, components of the complement, hydroly...

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Autores principales:	L. F. Zaynetdinova, L. F. Telesheva, A. V. Koryaushkina
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	external genital endometriosis local immunity human papillomavirus of high oncogenic risk ureaplasma infection Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/6af4c6f0540c4082a49e5a64fc0f83a4
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spelling	oai:doaj.org-article:6af4c6f0540c4082a49e5a64fc0f83a42021-11-22T07:09:54ZFeatures of local immunity in women with endometriosis and genital infection2220-76192313-739810.15789/2220-7619-FOL-1192https://doaj.org/article/6af4c6f0540c4082a49e5a64fc0f83a42020-04-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1192https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Introduction. External genital endometriosis is an inflammatory estrogen-dependent disease characterized by implantation and proliferation of endometrial tissue outside the uterus, accompanied by increased production of proinflammatory cytokines, prostaglandins, components of the complement, hydrolytic enzymes, increased angiogenesis and anomalies of ectopic endometrium. According to implantation theory, external genital endometriosis develops from viable endometrial cells transferred retrogradely through the fallopian tubes to the abdominal cavity during menstruation, while a disturbed local immunity is an important factor in its pathogenesis. Genital pathogens may be involved in the formation of the immune environment of the peritoneal cavity in women with endometriosis.Purpose. To study the peculiarities of local immunity in women with external genital endometriosis and genital infection pathogens.Materials and methods. 159 women with external genital endometriosis were examined. The total number of leukocytes, the absolute and relative number of viable cells, counts of neutrophils, macrophages and their functional activity, the level of IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFNα, IFNγ, TNFα in the peritoneal fluid were evaluated. The study of local immunity was performed in women with endometriosis, stage 1—2 and 3—4, depending on detected genital pathogens. Chlamydia trachomatis, Ureaplasma spp., Mycoplasma genitalium, HSV1,2/CMV, high carcinogenic risk HPV were analyzed by using PCR in samples collected from the endometrium, peritoneal fluid, and endometrioid heterotopies. Statistical processing was performed by using the IBM SPSS Statistics Version 22.2 statistical analysis software package.Results. In the presence of HPV and Ureaplasma spp. in women with endometriosis, stage 1—2, the decreased functional activity of peritoneal neutrophils and macrophages was found. At 3—4 stage, a correlation analysis revealed that detected HPV and Ureaplasma spp. obtained increased both pro-inflammatory and anti-inflammatory cytokines in the peritoneum. However, the higher activity of Th-2 cells in the peritoneal fluid secreting IL-4 and IL-10 and suppressing cellular immunity, was observed in HPV-positive samples. In addition, HPV also correlated with a decreased IL-2 and IL-4 levels.Conclusions. The most prominent changes in the immunological parameters from peritoneal fluid samples were observed in case of detected genital infection pathogens, particularly HPV. Thus, immune disturbances emerged upon bacterial and viral pathogen detection may contribute to the implantation of endometrial cells in the pelvic organs and disease progression.L. F. ZaynetdinovaL. F. TeleshevaA. V. KoryaushkinaSankt-Peterburg : NIIÈM imeni Pasteraarticleexternal genital endometriosislocal immunityhuman papillomavirus of high oncogenic riskureaplasma infectionInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 10, Iss 1, Pp 145-158 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	external genital endometriosis local immunity human papillomavirus of high oncogenic risk ureaplasma infection Infectious and parasitic diseases RC109-216
spellingShingle	external genital endometriosis local immunity human papillomavirus of high oncogenic risk ureaplasma infection Infectious and parasitic diseases RC109-216 L. F. Zaynetdinova L. F. Telesheva A. V. Koryaushkina Features of local immunity in women with endometriosis and genital infection
description	Introduction. External genital endometriosis is an inflammatory estrogen-dependent disease characterized by implantation and proliferation of endometrial tissue outside the uterus, accompanied by increased production of proinflammatory cytokines, prostaglandins, components of the complement, hydrolytic enzymes, increased angiogenesis and anomalies of ectopic endometrium. According to implantation theory, external genital endometriosis develops from viable endometrial cells transferred retrogradely through the fallopian tubes to the abdominal cavity during menstruation, while a disturbed local immunity is an important factor in its pathogenesis. Genital pathogens may be involved in the formation of the immune environment of the peritoneal cavity in women with endometriosis.Purpose. To study the peculiarities of local immunity in women with external genital endometriosis and genital infection pathogens.Materials and methods. 159 women with external genital endometriosis were examined. The total number of leukocytes, the absolute and relative number of viable cells, counts of neutrophils, macrophages and their functional activity, the level of IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFNα, IFNγ, TNFα in the peritoneal fluid were evaluated. The study of local immunity was performed in women with endometriosis, stage 1—2 and 3—4, depending on detected genital pathogens. Chlamydia trachomatis, Ureaplasma spp., Mycoplasma genitalium, HSV1,2/CMV, high carcinogenic risk HPV were analyzed by using PCR in samples collected from the endometrium, peritoneal fluid, and endometrioid heterotopies. Statistical processing was performed by using the IBM SPSS Statistics Version 22.2 statistical analysis software package.Results. In the presence of HPV and Ureaplasma spp. in women with endometriosis, stage 1—2, the decreased functional activity of peritoneal neutrophils and macrophages was found. At 3—4 stage, a correlation analysis revealed that detected HPV and Ureaplasma spp. obtained increased both pro-inflammatory and anti-inflammatory cytokines in the peritoneum. However, the higher activity of Th-2 cells in the peritoneal fluid secreting IL-4 and IL-10 and suppressing cellular immunity, was observed in HPV-positive samples. In addition, HPV also correlated with a decreased IL-2 and IL-4 levels.Conclusions. The most prominent changes in the immunological parameters from peritoneal fluid samples were observed in case of detected genital infection pathogens, particularly HPV. Thus, immune disturbances emerged upon bacterial and viral pathogen detection may contribute to the implantation of endometrial cells in the pelvic organs and disease progression.
format	article
author	L. F. Zaynetdinova L. F. Telesheva A. V. Koryaushkina
author_facet	L. F. Zaynetdinova L. F. Telesheva A. V. Koryaushkina
author_sort	L. F. Zaynetdinova
title	Features of local immunity in women with endometriosis and genital infection
title_short	Features of local immunity in women with endometriosis and genital infection
title_full	Features of local immunity in women with endometriosis and genital infection
title_fullStr	Features of local immunity in women with endometriosis and genital infection
title_full_unstemmed	Features of local immunity in women with endometriosis and genital infection
title_sort	features of local immunity in women with endometriosis and genital infection
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/6af4c6f0540c4082a49e5a64fc0f83a4
work_keys_str_mv	AT lfzaynetdinova featuresoflocalimmunityinwomenwithendometriosisandgenitalinfection AT lftelesheva featuresoflocalimmunityinwomenwithendometriosisandgenitalinfection AT avkoryaushkina featuresoflocalimmunityinwomenwithendometriosisandgenitalinfection
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Features of local immunity in women with endometriosis and genital infection

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